Hülya ILIKSU GÖZÜ, Dilek YAVUZER, Handan KAYA, Selahattin VURAL, Haluk SARGIN, Cem GEZEN, Mehmet SARGIN, Sema AKALIN

Fonksiyone tiroid nodüllerinde NIS ekspresyonundaki değişiklikler

Amaç: Bu çalışmada, otonom olarak fonksiyone tiroid nodüllerinde FTN ve ekstranodüler tiroid dokusunda sodyum-iyot simporter NIS protein ekspresyonunun seviyesi ve hücredeki yerleşim yeri araştırıldı. Bunun yanı sıra bu çalışmada hastaların klinik bulgularıyla human NIS hNIS protein ekspresyonu seviyeleri karşılaştırıldı.Hastalar ve Yöntemler: Histolojik kesitler, toksik multinodüler guatr nedeniyle ameliyat olmuş 28 hastaya 5 erkek, 23 kadın; ort. yaş 54.5±11.0 yıl; dağılım 37-72 yıl ait 36 otonom FTN ve 31 ekstranodüler tiroid dokusundan oluşmaktaydı. Parafine gömülmüş bu doku kesitlerinde, NIS protein ekspresyonu, labeled streptavidin-biotin yöntemi ile anti-hNIS monoklonal antikoru kullanılarak immünohistokimyasal olarak araştırıldı.Bulgular: Otonom fonksiyone tiroid nodüllerindeki hNIS pozitif hücrelerin oranı 13.33±12.09 ekstranodüler tiroid dokusuna 1.35±3.03 göre anlamlı olarak daha yüksekti. hNIS boyanması genellikle otonom FTN’lerde %88.9 ve ekstranodüler tiroid dokusunda %54.5 hücre zarı ile sınırlı idi. Klinik parametreler ve nodül volümü ile hNIS immünoreaktivitesi ilişkili bulunmadı.Sonuç: Bizim verilerimiz, fonksiyone nodüllerde ekstranodüler tiroid dokusuna göre daha fazla miktarda NIS protein ekspresyonu olduğunu gösterdi, fakat, hNIS immünoreaktivitesi literatürde daha önce bildirilenlere göre daha düşük seviyelerde bulundu. Bu durum değişik toplumlardaki kişisel farklılıklara ve mevcut iyodin durumuna bağlı olabilir. Ayrıca NIS proteininin hücredeki yerleşim yeri NIS’nin fonksiyonunun göstergesi olmayabilir

Anahtar Kelimeler:

Genetik transkripsiyon, sodyum-iyot simporter, tiroid bezi/cerrahi, tiroid tümörleri

Alterations of NIS expression in functioning thyroid nodules

Objectives: This study aimed to analyze both the level and the cell site of the sodium-iodide symporter NIS protein expression in autonomously functioning thyroid nodules AFTNs and extranodular thyroid tissues. In addition, this study sought to compare the clinical results of patients with the levels of human NIS hNIS protein expression. Patients and Methods: The histological slides consisted of 36 AFTNs and 31 extranodular thyroid tissues from 28 patients 5 males, 23 females; mean age 54.5±11.0 years; range 37 to 72 years who underwent surgery for toxic multinodular goitre. The expression of NIS protein was investigated by immunohistochemistry in paraffinembedded tissue sections using anti-hNIS monoclonal antibody by the labeled streptavidin-biotin method. Results: The percentage of hNIS positive follicular cells was significantly higher in the AFTNs 13.33±12.09 than in the extranodular thyroid tissues 1.35±3.03 . Staining for hNIS was mostly confined to the cell membrane in the AFTNs 88.9% and in the extranodular thyroid tissues 54.5% . The clinical parameters and nodule volume did not establish any correlation with hNIS immunoreactivity. Conclusion: Our data indicate that functioning nodules express higher amounts of NIS protein than the extranodular thyroid tissue, but the level of hNIS immunoreactivity was lower than had been reported in the previous literature. This result may be due to interindividual variability between different populations, and iodine status. Furthermore, the localization of the NIS protein might not give an indication of its functional status.

Keywords:

Genetic transcription, sodium-iodide symporter, thyroid gland/surgery, thyroid neoplasms,

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