The rapid increase in the development of world population and industrialization have brought many problems for human and environmental health. The widely use of chemicals in order to increase agricultural production is one of the main problems in this sense. To take into consideration this common problem, environmentally friendly production methods of organic farming as an alternative to conventional agriculture in different countries are put forward and accepted by both manufacturers and consumers, as well. In this study, the effects of different soil application forms and soil tillage techniques on the disease output of powdery mildew (Erysiphe necator) and grape yield in an organically managed vineyard with variety “Sultanina” were investigated. Soil applications were consisted of zeolite, farmyard manure, green manure, liquid fertilizer Bioplazma and E2001, a commercial organic biological suspension. The soil cultivation methods were mulching, use of chisel and the combination of plow + harrow. All the specified applications were conducted in combination with each other in the organically managed vineyard of the Research Institute of Viticulture, Manisa, for a period of three years. A vineyard in the vicinity of experimental vineyard which was managed with conventional cultivation methods was chosen to compare the results between organic and conventional systems. As in experimental organic vineyard, this vineyard was also visited at corresponding time intervals for disease monitoring and recording. The study has shown that zeolite application, depending upon the years, increased the incidence of powdery mildew. Application of organic fertilizers, due to stronger vegetative development, has resulted with more disease severity, changing with years, compared to the control. In the plots cultivated with chisel and mulching, a reduced disease severity was recorded in comparison with plow cultivation in both of organic and conventional plots.