Detection and Partial Molecular Characterization of Tomato chlorosis virus Isolates from Tomato Production Greenhouses in Antalya Province Using hsp70h Gene
Detection and Partial Molecular Characterization of Tomato chlorosis virus Isolates from Tomato Production Greenhouses in Antalya Province Using hsp70h Gene
Tomato chlorosis virus (ToCV) has been spreading in tomato production
area of the world, particularly in the Mediterranean countries in recent years.
The presence of the virus in Turkey was detected in Fethiye in 2008, but the
presence of the virus in most important tomato production region, Antalya
Province, is not yet known. In this study, a survey was conducted in Antalya
Merkez and Kumluca districts and 50 samples were collected from 12 different
tomato production greenhouses. After total nucleic acid isolation, all samples
were tested for the presence of the ToCV by amplification of the heat shock
protein 70 homologue gene (hsp70h) with gene-specific primers using nested RT-PCR
method. The nested PCR test revealed that 25 of 50 tomato samples (50%) were
infected with ToCV. These results showed the presence of ToCV in Antalya
province for the first time and demonstrated that the virus is widely
distributed in the region. The hsp70h genes of two isolates from Antalya Merkez
and Kumluca districts were sequenced and used for comparison and phylogenetic
analysis with each other and hsp70h genes of ToCV isolates from different
tomato production area of the world retriwed from GenBank databases. As results
of these analyses up to 5% genetic difference was determined among ToCV
isolates from different regions and ToCV isolates were phylogenetically divided
into two main groups. It was also determined that the phylogenetic groups
formed may be associated with geographical origin of ToCV isolates.
Keywords:
Tomato, hsp70h nested RT-PCR, sequence analysis,
___
- Albuquerque, L.C., Villanueva, F., Resende, R.O., Navas-Castillo, J., Barbosa, J.C., & Inoue-Nagata, A.K. 2013. Molecular characterization reveals Brazilian Tomato chlorosis virus to be closely related to a Greek isolate. Tropical Plant Pathology, 38(4):332-336. Arli-Sokmen, M., and Sevik M.A. 2006. Viruses infecting field-grown tomatoes in Samsun province, Turkey. Archives Phytopathology and Plant Protection 39(4):283-288. Çevik, B. and Erkış, G. 2008. First Report of Tomato Chlorosis Virus in Turkey. Plant Pathology 57(4):767 Delatte H., Naze F., Cottineau J.S., Lefeuvre P., Hostachy B., Reynaud B. and Lett J.M., 2006. Occurrence of Tomato chlorosis virus in Réunion Island. Plant Pathology 55: 289. Dovas, C.I., Katis, N.I. and Avgelis, A.V. 2002. Multiplex Detection of Criniviruses Associated With Epidemics of A Yellowing Disease of Tomato in Greece. Plant Diseases, 86(12), 1345-1349. Erkan, S., Gümüş, M., Türküsay, H., ve Duman, İ. 2001. Sanayi Domates Çeşitlerinin Bazı Viral ve Bakteriyel Hastalık Etmenlerine Karşı Davranışlarının Belirlenmesi. XI. Türkiye Fitopatoloji Kongresi, s:198-204. FAOSTAT, 2013. http://www.fao.org: 05.12.2016. Gharsallah, C., Halima, A. B., Fakhfakh, H., & Gorsane, F. (2015). Insights into the genetic diversity and the phylogenetic analysis of Tunisian isolates of Tomato chlorosis virus. Phytoparasitica, 43(1): 87-96. Hanssen I. M., Lapidot M. and Thomma B.P. 2010. Emerging viral diseases of tomato crops. Molecular plant-microbe interactions, 23(5):539-548. Hirota, T, Natsuaki, T, Murai, T, Nishigawa, H, Niibori, K Goto, K., Hartono, S. Suastika, G. and Okuda S. (2010). Yellowing disease of tomato caused by Tomato chlorosis virus newly recognized in Japan. Journal of General Plant Pathology 76:168-171. Jacquemond, M. Verdin, E. Dalmon, A. Guilbaud L. and Gognalons P. 2009Serological and molecular detection of Tomato chlorosis virus and tomato infectious chlorosis virus in tomato, Plant Pathology 58 (2): 210–220. Kataya, A.R.A., Stavridou, E., Farhan, K. and Livieratos, I.C. 2008. Nucleotide sequence analysis and detection of a Greek isolate of Tomato chlorosis virus. Plant Pathology: 57: 819–824. Lett, J.M., Hoareau, M., Reynaud, B., Saison, A., Hostachy, B., Lobin, K., and Benimadhu, S.P. 2009. First Report of Tomato chlorosis virus in tomato on Mauritius Island. Plant Dis. 9:111. Li R, Mock R, Huang Q, Abad J, Hartung J, 2008 A reliable and inexpensive method of nucleic acid extraction for the PCR-based detection of diverse plant pathogens. Journal of Virological Methods 154: 48-55. Louro, D., Accotto, G.P., Vaira, A.M. 2000. Occurrence and diagnosis of tomato chlorosis virus in Portugal. European Journal of Plant Pathology, 106(6):589-592. Lozano, G., Moriones, E., Navas-Castillo, J. 2006. Complete nucleotide sequence of the RNA2 of the crinivirus tomato chlorosis virus. Archives of Virology, 151(3):581-587. Martelli, G.P., Agranovsky, A.A., Bar-Joseph, M., Boscia, D., Candresse, T., Coutts, R.H., Dolja, V.V., Falk, B.W., Gonsalves, D., Jelkmann, W., Karasev, A.V., Minafra, A., Namba, S., Vetten, H.J., Wisler, G.C., Yoshikawa, N. 2002. The family Closteroviridae revised, Archives of Virology 147:2039–2044. Masse, D., Lefeuvre, P., Delatte, H., Abdoul-Karime A.L., Hostachy, B., Reynaud, B. and Lett, J.M. 2008. Tomato chlorosis virus: First report in Mayotte Island. Plant Pathology, 57(2). 388. Navas-Castillo, J., Camero, R., Bueno, M., Moriones, E. 2000 Severe yellowing outbreaks in tomato in Spain associated with infections of Tomato chlorosis virus. Plant Diseases, 84:835–837. Papayiannis, L.C., Ioannou, N., Dovas, C.I., Maliogka, V.I., Katis, N.I. 2005. First report of Tomato chlorosis virus (ToCV) on tomato crops in Cyprus. Plant Pathology 55: 567. Segev, L., Polston, J.E., Lapidot, M. 2004. First report of Tomato chlorosis virus in Israel. Plant Diseases 88: 160. Smith, K. M. 2012. A textbook of plant virus diseases. Elsevier.684 pp. Tsai, W.S., Shih, S.L., Gren, S.K., Hanson, P., Liu, H.Y. 2004. First report of the occurrence of Tomato chlorosis virus and Tomato infectious chlorosis virus in Taiwan. Plant Diseases 88: 311. Wintermantel, W. M., Polston, J. E., Escudero, J., Paoli, E.R. 2001. First report of Tomato chlorosis virus in Puerto Rico. Plant Diseases: 85, 228. Wintermantel, W.M. and Wisler, G.C. 2006. Vector specificity, host range, and genetic diversity of Tomato chlorosis virus. Plant Diseases. 90:814-819. Wisler, G.C., Li, R.H., Liu, H. Y., Lowry, D. S., Duffus, J.E. 1998. Tomato Chlorosis Virus., A New Whitefly-Transmitted,Phloem-Limited, Bipartite Closterovirus of Tomato The American Phytopathological Society. Vol. 88, No. 5, 402-409. Wisler, G.C., Duffus, J.E. 2001. Transmission properties of whitefly-borne criniviruses and their impact on virus epidemiology. In: Harris KF, Smith OP, Duffus JE (eds), Virus-insect–plant interactions. Academic Press, San Diego, pp 293–308. Zitter, T.A., Stall, R.E., Jones, J.P., 1997. Diseases caused by viruses. J. B. Jones, eds.) Compendium of Tomato Diseases, APS Pres, Minnesota, USA. pp. 31-42.
- ISSN: 0378-8024
- Yayın Aralığı: Yılda 3 Sayı
- Başlangıç: 1971
- Yayıncı: Türkiye Fitopatoloji Derneği
Sayıdaki Diğer Makaleler
Serkan ÖNDER, İsmail Can PAYLAN, Mustafa GÜMÜŞ