Environmental regulations imposed by world renowned associations have forced the global community to use eco-friendly and sustainable dyed products in applied fields. The current study has been concerned with the effect of ultrasonic radiation on the extraction of Hematein from logwood and its application onto wool fabric. The extraction was carried out by boiling respective amount of powder into methanolic medium, the extract obtained and wool fabrics were exposed to ultrasonic radiation for 15, 30, 45 and 60 min. Dyeing of irradiated and un-irradiated fabric was carried out using irradiated and un-irradiated extracts at 70 0C for 45 min. keeping material to liquor ratio of 1:25. For improvement in shades, salts of Al and Iron and tannic acid (TA) as chemical mordants and extracts of Zeera (Cuminum cyminum), Harmal (Peganum harmala), and Turmeric (Curcuma Longa) as source of bio mordants have been employed at 70 0C for 45 min. keeping material to liquor ratio of 1:25. It is found that extract after US treatment for 45 min. has given good colour strength onto un-irradiated wool. The colour strength results show that 1% Al, 3% Fe, 7% Tannic acid as pre- chemical mordants, where as 7% of Zeera, Harmal and Turmeric extracts as pre-bio-mordants have given good colour strength and excellent color coordinates, where as 1% Al, 7% Fe, 7% Tannic acid as post- chemical mordants, where as 3% of Zeera, 7% Harmal and 7% Turmeric extracts as post bio-mordants have given good colour characteristics at mild conditions. It is inferred that ultrasonic treatment for 45 min. has enhanced the coloring behavior of wool using hematein extracted from logwood chips.