Şeker Pancarı Posasından Elde Edilen Pektinin Modifiye Edilerek Karakterize Edilmesi, Moleküler ve Reolojik Özelliklerinin Belirlenmesi

Şeker sanayisinin önemli artıklarından olan şeker pancarı posasından optimum şartlarda (pH, süre ve sıcaklık) pektin elde edilmiştir. Sonraki aşamada pektin pektinaz enzimi kullanılarak modifiye edilmiştir. Modifikasyonunun pektinin yapısal özelliklerine etkisini gözlemleyebilmek için fourier dönüşümlü kızılötesi spektroskopisi (FT-IR) ve yüksek performanslı sıvı kromotografisi (HPLC) ile analizler gerçekleştirilmiştir. FT-IR analizinde pektinin spesifik bölgesi olan ve kalitesini belirleyen 1600-1800 cm-1 bantlarına bakılmıştır. HPLC analizinde ise galakturonik asit içeriğine bakılmıştır. Elde edilen sonuçlar incelendiğinde modifiye edilen pektinin galakturonik asit içeriğinin daha yüksek olduğu belirlenmiştir. Belirtilen bu analizlere ilave olarak elde edilen pektinlerin akış davranışları da belirlenmiştir. Modifiye edilen pektinin akış özelliklerinin değiştiği görülmüştür

Rheological and Molecular Characterization of Modified Pectin from Sugar Beet Meal

The pectin was produced from sugar beet pulp, one of the significant waste of sugar industry, at optimum conditions (pH,time and temperature). Then, the modification was carried out using pectinase. FT-IR (Fourier transform infrared spectroscopy) and HPLC (high performance liquid chromatography) analysis were performed to characterize the differences between modified and unmodified pectin. FT -IR analysis revealed the specific regions of pectin and 1600-1800 cm-1 bands. Galacturonic acid content was analyzed by HPLC. The obtained results revealed that the modified pectin had higher galacturonic acid content. In addition, flow properties of the modified pectin was also determined. The flow characteristics of modified pectin was observed to change

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