O. YORGANCİLAR, I. KUTLU, A. YORGANCİLAR, P. UZUN

Anther Culture Response to Different Media in F2 Progenies of Bread Wheat (Triticum aestivum L.)

Anter kültürü, double haploid bitki elde etmek için kullanılan en önemli yöntemlerden birisidir ve bu yöntemin etkinliği, genotip ve kültür ortamı gibi pek çok etmenden etkilenir. Bu çalışmada, MN6 ve P2 ortamları kullanılarak, farklı ekmeklik buğday melezi F2 döllerinin anter kültürüne tepkisi araştırılmıştır. Sonuçlar, bütün genotiplerde kallus üretimi, rejenerasyon, yeşil ve albino sayısının, P2ye göre MN6 ortamında daha yüksek olduğunu göstermiştir. Buna ek olarak, incelenen tüm özellikler bakımından genotipler arasında farklılıklar vardır ve anter kültürüne tepkinin büyük oranda hem genotipten hem de kültür ortamından etkilendiğini ve kullanılan melezler için en uygun kültür ortamının MN6 ortamı olduğu söylenebilir. Ancak, etkin bir ıslah programında bu populasyonun kullanabilmesi diğer kültür koşullarının da geliştirilmesiyle olasıdır.

Ekmeklik Buğdayın (Triticum aestivum L.) F2 Döllerinin Farklı Ortamlarda Anter Kültürüne Tepkisi

The anther culture is one of the most important methods for producing doubled haploid plants and the efficiency of this method is influenced by several mentioned factors such as genotype and induction media. In this study, it was investigated that anther response of different F2 progenies of bread wheat (Triticum aestivum L.) hybrids using MN6 and P2 induction media. The results indicated that callus production, regeneration, green and albino plant numbers are higher on MN6 media than on P2 media for all genotypes. In addition to, all investigated parameters varied between genotypes. It can be said that the response of anthers depends mainly both the genotype and media, and the most suitable induction media for obtaining doubled haploid from our wheat hybrids was MN6 medium. However, it may be needed to develop other culture conditions for this population to utilize it in an actual breeding program.

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