Uzun yapraklı üçgülün (Trifolium pannonicum ssp. elongatum) hipokotil ve yaprak sapı eksplantından in vitro çoğaltılması

Bu çalışmada, kinetin ve α- naftalin asetik asitin (NAA)’in farklı konsantrasyonlarının (kinetin ve 0.1+ 0.1, 0.2, 0.4 NAA mg/l), uzun yapraklı üçgül (Trifolium pannonicum ssp. elongatum) bitkisinin hipokotil ve kotiledon yaprak sapı eksplantlarından gelişen sürgün yüzdesi, sürgün sayısı ve sürgün uzunluğu üzerine olan etkileri araştırılmıştır. Araştırmada, sürgün oluşturan eksplant yüzdesinin en yüksek oranda (% 91.7) hipokotil eksplantından ve Murashige and Skoog (MS) içinde 1 mg/l Kinetin + 0.4 mg/l NAA içeren ortamdan; en düşük sürgün yüzdesinin (% 25.0) hipokotil ve kotiledon yaprak sapı eksplantlarının her ikisinde de 1 mg/l Kinetin + 0.1 NAA içeren rejenerasyon ortamında geliştiği belirlenmiştir. Eksplant basına en fazla sürgün sayısı 11.2±1.3 adet ile 1 mg/l Kinetin+0.4 mg/l NAA içeren ortamdan, en düşük sürgün sayısı 2.5±0.6 adet ile 1 mg/l kinetin + 0.1 NAA uygulamasında gözlenmiştir. En uzun sürgün gelişimi 3.5±0.5 cm ile (1 mg/l kinetin+ 0,1 mg/L NAA) içeren MS ortamında, en kısa sürgün gelişimi ise 1.5±0.2 cm ile 1 mg/l kinetin + 0.4 NAA MS ortamında belirlenmiştir. Trifolium pannonicum JACQ. ssp. elongatum bitkisinden maksimum kök gelişimi % 95±2.9 ile 1 mg/l IBA uygulamasından elde edilmiştir.

In vitro propagation of long leaved clover (Trifolium pannonicum ssp. elongatum) using hypocotyle and petiole Explants

The study reports effects of various concentrations of Kinetin and α- Naftalin asetik asit (NAA) (kinetin and 0.1+ 0.1, 0.2, 0.4 NAA mg/l) on shoot regeneration (percentage, number and length of shoot) of Trifolium pannonicum ssp. elongatum from hypocotyl and petiole of cotyledon leaf. The maximum (91.7%) shoot regeneration frequency (25%) was recorded on hypocotyl explant on Murashige and Skoog (MS) medium containing 1 mg/l Kinetin + 0.4 mg/l NAA; whereas, minimum shoot regeneration frequency (25%) was in medium containing 1 mg/l Kinetin + 0.1 NAA respectively, in both hypcotyl and petiole of cotyledon leaf explant. The highest number of 11.2±1.3 shoots per explant was recorded on MS medium containing 1 mg/l Kinetin+0.4 mg/l NAA and the lowest number of 2.5±0.6 was in medium containing 1 mg/l Kinetin+0.1 mg/l NAA respectively. The longest shoots (3.5±0.5 cm) were recorded on MS medium containing 1 mg/l kinetin+ 0,1 mg/L NAA, and the smallest shoots (1.5±0.2 cm) were recorded on MS medium containing 1 mg/l kinetin + 0.4 NAA. Maximum frequency of 95% roots was recorded on MS medium containing 1 mg/l IBA.

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