Hasan MEYDAN, Hans CHENG, Mehmet Ali YILDIZ

Tavuklarda Marek Hastalığı'na Karşı Dirençli Genlerin SNP Genetik Markerlerinden Yararlanılarak Belirlenmesi

Tavukçuluk endüstrisini tehdit eden hastalıklardan birisi olan Marek Hastalığı (MH), alfa-herpesviruslerden biri olan Marek Hastalık Virüsü (MHV)nün neden olduğu, bulaşıcı ve kanserojen bir hastalıktır. MHye karşı dayanıklılığı arttırmak için başlıca yöntem günlük civcivlerin aşılanmasıdır. Aşılama sonucunda bağışıklık sisteminde meydana gelen yetersizliklerden dolayı MH ile mücadelede diğer bir yöntem MHye genetik olarak dirençli tavukların yetiştirilmesidir. Bu çalışmada, tavuklarda ENO1, TPT1 ve RBBP4 genlerinin MHye karşı dayanıklılığa katkı sağlayıp sağlamadığı allele özgü ekspresyon tekniği kullanılarak araştırılmıştır. Araştırmada, materyal olarak MHye dayanıklı (hat 6) ve duyarlı (hat 7) tavuk hatlarının resiprokal melezlenmesi sonucunda elde edilen F1 dölleri kullanılmıştır. Tavukların Bursa fabricius, timus ve dalak organlarından RNA izole edilmiş ve pirosekanslama yöntemi ile her bir allelin ekspresyon seviyesi belirlenmiştir. Araştırma sonuçları, TPT1 ve RBBP4 genlerinin cis-acting elemente sahip olduğunu, ancak bu cis-acting elementlerin MHna karşı direnç oluşturmada rol oynamadığını ortaya koymuştur. Anahtar Kelimeler: ENO1; TPT1; RBBP4; ASE; Pirosekanslama; Genetik dirençlilik

Identification of the Genes that Confer Genetic Resistance to Marek s Disease in Chicken Using SNP Markers

Marek s disease (MD), one of the most threatening diseases to poultry industry, is contagious disease of domestic chickens induced by the Marek s disease virus (MDV), a highly infectious and naturally oncogenic alphaherpesvirus. Enhancing resistance to MD in poultry is an attractive method to augment MD vaccines. Because the inadequacy in immune system occurs as a result of vaccine, the other method for avoiding from MD is to breed the chickens that are resistant to MD. In this study, ENO1, TPT1 and RBBP4 genes in chickens were screened whether they played a role in resistance to MD by using allele-specific expression (ASE). The F1 progeny of reciprocal intermatings between highly inbred chicken line 6 (MD resistant) and line 7 (MD susceptible) were sampled for this study. RNA was isolated from three organ tissues (Bursa fabricius, thymus and spleen) and the relative expression levels of each allele were monitored by using pyrosequencing method. The results suggested that TPT1 and RBBP4 genes have the cis-acting regulatory elements but these cis-acting elements do not play any role in conferring genetic resistance to MD.

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