Mustafa OCAK, Orçun Burak ŞAVUR, Bülent POLAT, Mustafa USTA, Hikmet Murat SİPAHİOĞLU

Prunus yapraklarında prunus necrotic ring spot (PNRSV) ve apple chlorotic leaf spot (ACLSV) virüslerinin dağılımı

Prunus necrotic ringspot virüs (PNRSV)'ü ile infekteli Prunus mahaleb ve Apple chlorotic leafspot virüs (ACLSV)'ü ile infekteli şeftali (P. persica L.) yapraklarının farklı bölgelerinden alınan doku diskleri bu virüslerin yaprak dokusundaki dağılımlarını belirlemek amacı ile sırasıyla enzyme-linked immunosorbent assay (ELISA) ve reverse transcriptase polymerase chain reaction (RT-PCR) teknikleri ile analiz edilmişlerdir. Gerçekleştirilen ELISA testleri sonucunda her iki virüsünde yaprak ayasında yaprak sapı bölgesinde daha konsantre oldukları ve konukçu yapraklarında düzensiz bir dağılım gösterdikleri tespit edilmiştir. Aynı yaprak bölgelerinin kullanıldığı RT-PCR testlerinde 'ise her iki virüsün genetik materyalinin tüm yaprak bölgeleri için birbirine yakın ölçülerde amplifikasyon ürünleri oluşturduğu belirlenmiş ve testlenen yaprak bölgeleri arasında viral konsantrasyon bakımından bariz farklılıkların olmadığı saptanmıştır. RT-PCR testi sonuçlarından elde edilen kesin, net ve dengeli teşhisi ifade eden bantlar, ACLSV ve PNRSV virüslerinin Prunus yapraklarının testlenen tüm bölgelerinde homojen bir dağılım sergilediğini göstermiştir. Her iki virüs, kullanılan test yöntemine göre konukçularında farklı dağılım sergilemişlerdir. Elde edilen bulgular ışığında PNRSV ile ACLSV'nin konukçularındaki dağılımını belirlemede ELISA testi ile PCR testi arasında bir korelasyon saptanmamıştır.

Distribution of prunus necrotic ringspot (PNRSV) and apple chlorotic leaf spot viruses (ACLSV) in Prunus leaves

Leaf discs taken from different canopy and leaf sites of Prunus necrotic ringspot virus (PNRSV) infected Prunus mahaleb and Apple chlorotic leafspot virus (ACLSV) infected peach (P. persica) tree were analyzed for the determination of virus distribution in leaf tissues by enzyme-linked immunosorbent assay (ELISA) and reverse transcriptase polymerase chain reaction (RT-PCR) techniques. The ELISA results suggest high virus concentration of PNRSV and ACLSV at the basal leaf section of the lamina and uneven virus distribution in their host leaves. Clear and relatively balanced amplification bands were obtained when the same leaf sections were analyzed by RT-PCR. No conspicuous differences were found in terms of viral concentrations among the leaves tested. Amplification bands of RT-PCR test results suggest homogeneous distribution of both viruses in tested Prunus leaves. Both viruses were exhibited different patterns of distribution in their hosts according to the detection method used. No correlation was found between ELISA and RT-PCR tests in determining the distribution of both viruses.

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