Kahraman GÜRCAN, Adem TAŞ, Nihal DENLİ, Halit YETİŞİR

Morphological Characterization of Bottle Gourd (Lagenaria siceraria (Molina) Standl.) Germplasm and Formation of a Core Collection

Bottle gourd [Lagenaria siceraria (Molina) Standl.] genotypes were collected from different parts of Turkey and a corecollection (CC) was established based on morphological characteristics. The collection was grown for morphologicalcharacterization and seed multiplication. Seeds were produced from 322 genotypes (Entire Population- EP) representingdiversity of entire country by selfing and the accessions were characterized for 25 quantitative and 21 qualitativemorphological characteristics. Important variation was observed in fruit size, cotyledon dimensions, leaf dimension,flower size, fruit length (8.7-155.7 cm), fruit shape (flat-elongate), seed weight (3.8 g/100 seeds -38 g/100 seeds)among accessions as previously reported. UPGMA cluster, and principal component analyses cluster of quantitative andqualitative characteristics of EP were assayed. Results of the assays together with geographic origin of the genotypeswere analyzed to establish a core population (CC) consisting of 100 genotypes. High correlation between the CC and theEP (r= 0.974 for normalized Mantel Statistic Z) demonstrated that CC represents the most of total genetic variation withminimum redundancy in bottle gourd germplasm of Turkey. The CC will enable efficient and cost-effective managementand utilization of bottle gourd germplasm.

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Balkaya A, Yanmaz R & Özbakir M (2009). Evaluation of variation in seed characters of Turkish winter squash (Cucurbita maxima) populations. New Zealand Journal of Crop and Horticultural Science 37(3): 167-178
Biodiversity International for Cucurbitaceae (2007). Guidelines for the development of crop descriptor lists. Bioversity Technical Bulletin Series. Biodiversity International, Rome, Italy
Brown A H D (1989). Core collections: a practical approach to genetic resources management. Genome 31(4): 818-824
De Cristo-araújo M, Rodrigues D P, Astolfi-filho S & Clement C R (2015). Peach palm core collection in Brazilian Amazonia. Crop Breeding and Applied Biotechnology 15: 18-25
Decker-Walters D S, Wilkins-Ellert M, Chung S M & Staub J E (2004). Discovery and genetic assessment of wild bottle gourd [Lagenaria siceraria (Mol.) Standley; Cucurbitaceae] from Zimbabwe. Economic Botany 58(4): 501-508
Frankel O H (1984). Genetic perspectives of germplasm conservation. In Genetic Manipulation: Impact on Man and Society (W.K. Arber, K. Llimensee, W.J. Peacock and P. Starlinger, Eds.). Cambridge University Press, Cambridge, UK, pp. 161-170
Given D R (1987). What the conservationist requires of ex situ collections. In: Branwell D, Hamann O, Heywood V, Synge H., editors. Botanic gardens and the world conservation strategy. Academic Press, London, pp. 103-116
Gürcan K, Say A, Yetisir H & Denli N (2015). A study of genetic diversity in bottle gourd [Lagenaria siceraria (Molina) Standl.] population, and implication for the historical origins on bottle gourds in Turkey. Genetic Resources Crop Evolution 62(3): 321-333
Kistler L, Montenegro A, Smith B D, Grifford J A, Green R E, Newsom L A & Shapiro B (2014). Transoceanic drift and the domestication of African bottle gourds in the Americas. Proceedings of the National Academy of Sciences of the United States of America 111: 2937-2941
Loukou A L, Gnakri D, Djè Y, Kippré A V, Malice M J P B, Baudoin J-P & Zoro Bi I A (2007). Marie et al. Page 53 Macronutrient composition of three cucurbit species cultivated for seed consumption in Côte d’Ivoire. African Journal of Biotechnology 6: 529-533
Mashilo J, Shimelis H A, Odindoa A O & Amelework B A (2017). Genetic differentiation of bottle gourd [Lagenaria siceraria (Molina) Standl.] landraces assessed by fruit qualitative traits and simple sequence repeat markers. Scientia Horticulturae 216: 1-11
Mladenovic E, Berenji J, Ognjanov V, Ljubojević M & Čukanović A (2012). Genetic variability of bottle gourd (Lagenaria siceraria (Mol.) Standley) and its morphological characterization by multivariate analysis. Archives of Biological Sciences 64(2): 573-583
Morimoto Y, Maundu P, Fujimaki H & Morishima H (2005). Diversity of landraces of the white-flowered gourd (Lagenaria siceraria) and its wild relatives in Kenya: fruit and seed morphology. Genetic Resources and Crop Evolution 52(6): 737-747
Paris H S (2001). Characterization of Cucurbita pepo collection at the Newe Ya’ar Research Center, Israël. Plant Genetic Resources Newsletter 126: 41-45
Rodrigues D P, Astolfi-Filho S & Clement C R (2004). Molecular marker mediated validation of morphologically defined landraces of Pejibaye (Bactris gasipaes) and their phylogenetic relationships. Genetic Resources and Crop Evolution 51: 871-882
Rohlf F J (2000). NTSYS-pc. Numerical taxonomy and multivariate analysis system, version 2.1. Exeter Software, Setauket, New York, USA
Schlumbaum A & Vandorpe P (2012). A short history of Lagenaria siceraria (bottle gourd) in the Roman provinces: morphotypes and archaeogenetics. Vegetation History and Archaeobotany 21(6): 499- 509
van Hintum T J L (1999). The general methodology for creating a core collection. In Johnson RC and Hodgkin T (Eds.) Core collections for today and tomorrow. IPGRI, Roma, pp. 10-17
Yetisir H & Uygur V (2009). Plant growth and mineral element content of different gourd species and watermelon under salinity stress. Turkish Journal of Agriculture and Forestry 33(1): 65-77
Yetisir H, Sari N & Yücel S (2003). Rootstock resistance to Fusarium wilt and effect on fruit yield and quality of watermelon. Phytoparasitica 31(2): 163-169
Yetisir H, Sakar M & Serce S (2008). Collection and morphological characterization of Legenaria siceraria germplasm form the Mediterranean Region of Turkey. Genetic Resources and Crop Evolution 55(8): 1257- 1266