Serhan MERMER, Melis YALÇIN, Leyla Didem KOZACI, Cafer TURGUT

Evaluation of Insecticide Toxicity and Enzymatic Detoxification in Neonate Larvae of European Grapevine Moth, Lobesia botrana Denis & Schiff. (Lepidoptera: Tortricidae)

Lobesia botrana Denis & Schiff. (Lepidoptera: Tortricidae), also known as the European grapevine moth is a detrimental pest in grape production in Palearctic Region. Insecticides are used to control L. botrana infestation in grape-produced areas; however, repeated and intensive use of insecticide causes reduced efficacy in L. botrana management. In the present study, the efficacy of five commonly used insecticides (chlorpyrifos-ethyl, emamectin benzoate, indoxacarb, lambda-cyhalothrin, and spinosad) was evaluated in two field populations (AL and SAR) from Manisa, Turkey. In addition, detoxification enzyme activities including esterase (EST) and glutathion-S-transferase (GST) were measured via in vitro assays. LC50 values were found lower for chlorpyrifos-ethyl, emamectin benzoate, and spinosad and higher for indoxacarb and lambda-cyhalotrin in AL populations compared to SAR population. EST levels were slightly higher in AL population compared to SAR. GST levels were found higher in SAR population. However, no statistical difference was found in both detoxification enzyme activities and EST enzyme levels were higher than GST enzyme levels in both populations. Findings of the current study would help growers as well as the applicator to strategize their insecticide use in integrated pest management programs for L. botrana and possibly mitigate any insecticide resistance development.

Keywords:

detoxification enzyme dose-response, grape, insecticide toxicity, Tortricidae,

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