Efficacy of Native Entomopathogenic Nematodes on the Larvae of Tomato Leafminer Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)

Efficacy of Native Entomopathogenic Nematodes on the Larvae of Tomato Leafminer Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)

This study was conducted to determine the efficacy of nativeentomopathogenic nematodes (EPNs); Steinernema affine 46(Bovien, 1937), S. feltiae 879 (Filipjev, 1934), S. carpocapsae1133 (Weiser, 1955) and Heterorhabditis bacteriophora 1144(Poinar, 1976) on the larvae of tomato leafminer Tuta absoluta(Meyrick). Bioassays were conducted in the laboratory at fourdifferent temperatures (10, 15, 20 and 25±1 oC) in the plates and30 infective juveniles (IJs) were inoculated to a single T. absolutalarva for each nematode species. After nematode inoculation,larvae were checked on the 3rd, 5th, 7th days and mortalities wererecorded. All nematode species used in the study showed thelowest efficacy on the 3rd control day at 10 oC and the highestefficacy on the 7th day at 25 oC. S. feltiae 879 was found as themost efficient species with the highest mortality (91.67%) amongEPNs used in the study. The results proved that T. absoluta larvaeare highly susceptible to EPNs and the control of the pest byEPNs on this stage is successful.

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  • Batalla-Carrera L, Morton A & Garcia-del-pino F (2010). Efficacy of entomopathogenic nematodes against the tomato leafminer Tuta absoluta in laboratory and greenhouse conditions. Biocontrol 55: 523-530
  • Bedding R A & Akhurst R J (1975). A simple technique for the detection of insect parasitic Rhabditid nematodes in soil. Nematologica 21: 109-110
  • Ben Husin T O A (2017). Biological control of tomato leaf miner Tuta absoluta using entomopathogenic nematodes. PhD Thesis, Faculty of Science, Agriculture and Engineering, Newcastle University, United Kingdom, pp. 196
  • Boemare N (2002). Biology, taxonomy and systematic of Photorhabdus and Xenorhabdus. In: R. Gaugler (Ed.), Entomopathogenic Nematology. Wallingford, UK: CAB International, pp. 35-56
  • Boemare N E, Akhurst R J & Mourant R G (1993). DNA relatedness between Xenorhabdus spp. (Enterobacteriaceae), symbiotic bacteria of entomopathogenic nematodes, and a proposal to transfer Xenorhabdus luminescens to a new genus, Photorhabdus gen. nov. International Journal of Systematic Bacteriology 43(2): 249-255
  • Cáceres S (1992). La polilla del tomate en Corrientes. Biología y control, Estación Experimental Agropecuaria Bella Vista INTA, pp. 19
  • Desneux N, Wajnberg E, Wyckhuys K A G, Burgio G, Arpaia S, Narvaez-Vasquez C A, Gonzalez-Cabrera J, Ruescas D A, Tabone E, Frandon J, Pizzol J, Poncet C, Cabello T & Urbaneja A (2010). Biological invasion of European tomato crops by Tuta absoluta: Ecology, geo-graphic expansion and prospects for biological control. Journal of Pest Science 83: 197- 215
  • Ehlers R U (1996). Current and future use of nematodes in biocontrol: Practice and commercial aspects in regard to regulatory policies. Biocontrol Science Technology 6: 303-316
  • Estay P (2000). Polilla del tomate Tuta absoluta (Meyrick), URL http://alerce.inia.cl/docs/Informativos/Informativo09.pdf (Date accessed: September 2014)
  • Garcia-del-Pino F, Alabern X & Morton A (2013). Efficacy of soil treatments of entomopathogenic nematodes against the larvae, pupae and adults of Tuta absoluta and their interaction with the insecticides used against this insect. BioControl 58(6): 723-731
  • Gözel Ç (2016). Domates güvesi Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)’nin mücadelesinde entomopatojen nematodların kullanım olanakları. Çanakkale Onsekiz Mart Üniversitesi Fen Bilimleri Enstitüsü Doktora Tezi, 83 s
  • Gözel Ç & Kasap İ (2015). Efficacy of entomopathogenic nematodes against the tomato leafminer, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) in tomato field. Turkish Journal of Entomology 39(3): 229-237
  • Gözel U & Güneş Ç (2013). Effect of entomopathogenic nematode species on the corn stalk borer (Sesamia cretica Led. Lepidoptera: Noctuidae) at different temperatures. Turkish Journal of Entomology 37(1): 65-72
  • Kamali S, Karimi J & Koppenhöfer A M (2018). New Insight into the Management of the Tomato Leaf Miner, Tuta absoluta (Lepidoptera: Gelechiidae) with Entomopathogenic Nematodes. Journal of Economic Entomology 111(1): 112-119
  • Kasap İ, Gözel U & Özpınar A (2011). “Yeni bir zararlı; Domates güvesi Tuta absoluta (Meyrick, 1917) (Lepidoptera: Gelechiidae), 284”. Çanakkale Tarımı Sempozyumu (Dünü, Bugünü, Geleceği) Bildirileri), 10-11 Ocak Çanakkale Onsekiz Mart Üniversitesi Ziraat Fakültesi, Çanakkale pp: 717
  • Kaya H K & Gaugler R (1993). Entomopathogenic nematodes. Annual Review of Entomology 38: 181-206
  • Kaya H K & Stock S P (1997). “Techniques in Insect Nematology, 281-324” In: L A Lacey (Ed.), Manual of Techniques in Insect Pathology Biological Techniques Series, Academic Press, San Diego, California pp: 409
  • Kılıç T (2010). First record of Tuta absoluta in Turkey. Phytoparasitica 38: 243-244
  • Lacey L A & Unruh T R (1998). Entomopathogenic nematodes for control of codling moth, Cydia pomonella (Lepidoptera: Tortricidae): Effect of nematode species, concentration, temperature, and humidity. Biological Control 13: 190-197
  • Olthof ThHA & Broadbent A B (1990). “Control of a chrysanthemum leafminer, Liriomyza trifolii with the entomophilic nematode, Heterorhabditis heliothidis, 379”. Abstracts of Papers, Posters and Films Presented at the Second International Nematology Congress, 11-17 August, Veldhoven the Netherlands, Nematologica 36(1990): 327-403
  • Roditakis E, Papachristos D & Roditakis N E (2010). Current status of the tomato leafminer Tuta absoluta in Greece. EPPO Bulletin 40: 163-166
  • Shamseldean M, Abd-Elbary N A, Shalaby H & Ibraheem H I H (2014). Entomopathogenic nematodes as biocontrol agents of the tomato leaf miner Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) on tomato plants. Egyptian Society for Biological Control of Pests 24: 503-513
  • Siqueira H A A, Guedes R N C & Picanço M C (2000). Insecticide resistance in populations of Tuta absoluta (Lepidoptera: Gelechiidae). Agricultural and Forest Entomology 2: 147-153
  • Susurluk A & Ehlers R U (2008). Field persistence of the entomopathogenic nematode Heterorhabditis bacteriophora in different crops. BioControl 53: 627-641
  • Susurluk A, Dix I, Stackebrandt E, Strauch O, Wyss U & Ehlers R U (2001). Identification and ecological characterisation of three entomopathogenic nematode-bacterium complex from Turkey, Nematology 3(8): 833-841
  • Türköz S & Kaşkavalcı G (2016). Determination of the efficacy of some entomopathogenic nematodes against Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) under laboratory conditions. Turkish Journal of Entomology 40(2): 175-183
  • Urbaneja A, Vercher R, Navarro V, Porcuna J L & Maria F G (2007). La polilla del tomate, Tuta absoluta. Phytoma España 194: 16-23
  • Van Damme V M, Beck B K, Berckmoes E, Moerkens R, Wittemans L, Vis R D, Nuyttens D, Casteels H F, Maes M, Tirry L & Clercq P D (2016). Efficacy of entomopathogenic nematodes against larvae of Tuta absoluta in the laboratory. Pest Management Science 72: 1702-1709
  • Vargas H C (1970). Observaciones sobre la biologia y enemigos naturales de la polilla del tomate, Gnorimoschema absoluta (Meyrick) (Lepidoptera: Gelechiidae). Idesia 1: 75-110
  • White G F (1927). A method for obtaining infective nematode larvae from cultures. Science 66: 302-303