Determination of amitraz (Varroaset) residue in honey by high performance liquid chromatography (HPLC)

Amitraz (Varroaset) bal arılarında (Apis mellifera L.) (Hymenoptera: Apidae) sorun olan Varroa destructor Anderson & Trueman (Acarina: Varroidae) 'e karşı kullanılan bir akarisittir. Ballardaki amitraz kalıntısı HPLC ile tespit edilmiştir. Bal örnekleri arı yetiştiricilerinden Ankara'nın ilçeleri olan Ayaş, Kızılcahamam ve Polatlı'dan hasat dönemi boyunca toplanmıştır. Toplanan 32 örnekten 6 ( %15 ) adedinde amitraz kalıntısına rastlanılmıştır. Örneklerde tespit edilen kalıntı miktarları sırasıyla 5.35, 0.34, 0.23, 1.27, 0.92 ve 0.40 ppm. dir.Tespit edilen kalıntı limiti 0.0643 ppm ve elde edilme oranı %56,71 dir. Bu sonuç doğrultusunda Dünya Sağlık Örgütü (WHO)' nün izin vermiş olduğu limit 1 mg/kg iken bazı bal örneklerinde daha yüksek düzeyde amitraz kalıntısı olduğu görülmektedir.

Ballarda amitraz (Varroaset) kalıntısının yüksek performans likit kromatografi yöntemi (HPLC) ile belirlenmesi

Amitraz (Varroaset) is an acaricide applied against Varroa destructor Anderson & Trueman (Acarina: Varroidae) infestations on honeybees (Apis mellifera L.) (Hymenoptera: Apidae). Amitraz residue in honey was determined by HPLC in Ankara, Turkey. Honey samples were collected from beekeepers during the harvesting period in AyaĢ, Kızılcahamam and Polatlı towns in Ankara. Amitraz residue was found in various levels in six (15%) out of 32 honey samples. The residue amounts in the 6 samples were 5.35, 0.34, 0.23, 1.27, 0.92 and 0.40 ppm. The limit of detection was 0.0643 ppm and the recovery ratio was 56.71 %. According to the results, some honey samples showed higher residue levels of amitraz than the World Health Organisation (WHO) limit of 1 mg/kg.

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