DİLEK BAYRAM, Alpaslan GÖKÇİMEN, Meral ÖNCÜ, İbrahim Aydın CANDAN, Meltem ÖZGÖÇMEN

Silybum marianum bitkisinin in vitro ortamda karaciğer kanseri hücre hatları üzerine etkisi

Amaç: Hepatosellüler Karsinom (HSK) karaciğerin en sık (%75) rastlanan primer tümörüdür. HSK'nin etkin bir tedavisi yoktur. HSK'da radyoterapi ve kemoterapi tedavisi ile genellikle başarılı sonuçlar alınamamaktadır. Hep3B, hepatit B virüsü pozitif ve p53 geni mutasyona uğramış hepatosellüler karsinoma hücre soyudur. Silibin devedikeni (Silybum marianum )bitkisinden elde edilen bir ekstraktır. Yapılan çalışmalarda silibinin akciğer tümörü büyümesini baskıladığı, meme, kolon, mesane kanseri hücre hatlarında hücre siklusunu durdurduğu ve apopitozisi tetiklediği gösterilmiştir. Çalışmamızda silibinin Hep3B hücre soyu üzerine etkisini hem monolayer hem de siferoid kültür ortamında incelemeyi amaçladık. Gereç Yöntem: Çalışmamızda silibinin Hep3B hücre soyu üzerine etkisini hem monolayer hem de siferoid kültür ortamında incelemek amacıyla 100 µM/ml doz silibin hücrelere uygulandı. Silibin (100 µM/ml) uygulandıktan sonra 24, 48 ve 72 saat süreler sonunda BrdU işaretleme indeksi ve aktif kaspaz-3 tayinine immünohistokimyasal yöntemle bakıldı. Bulgular: Sonuç olarak silibinin hem monolayer hem de sferoid kültürde BrdU ile işaretlenen hücrelerin sayısını azalttığı, kaspaz-3 ile boyanan hücrelerin sayısını ise arttırdığı görüldü. Buradan da anlaşılacağı üzere silibin Hep3B hepatosellüler karsinoma hücre soyunda hücre proliferasyonunu inhibe edici ve apoptozisi tetikleyici etki göstermiştir. Üç boyutlu sferoid model vücuttaki tümör yapısını daha iyi yansıttığı için elde ettiğimiz sonuçların hepatosellüler karsinoma tedavisine ışık tutacağı kanısındayız

Effect of silybum marianum plant on liver cancer cell lines: an in vitro study

Aim: Hepatocellular carsinoma (HCC) is the most seen primer tumor in liver ( 75%). There isn’t exact cure for HCC. No successful results can be obtained with the treatment of chemotherapy, radiotheraphy in liver cancer. Hep3B cell line is hepatit B virus positive and p53 gene-mutated hepatocarcinoma cell line. Silibinin is extracted from milk thistle (Silybum marianum). Previous studies showed extracts of plant suppressed lung tumor growth, interrupted cell cycle and promoted apopitosis on breast, colon and bladder cancer cell lines. In our study we aimed to investigate the effect of silibinin on Hep3B cell line in monolayer and spherid culture. Material Method: In this study the effect of silibinin on Hep3B cell line in both monolayer and spheroid in the culture medium to examine the 100 microM/ml dose silibinin was applied. After Silibinin (100 microM/ml) applying 24, 48 and 72 hour periods of time marking the end of BrdU labelling index and the determination of active caspase-3 were measured by immunohistochemistry. Results: In conclusion, silibinin reduced the number of cells marked with BrDU, increase the number of cells stained with caspase-3 in both monolayer culture and spheroid culture. Silibin Hep3B hepatocellular carcinoma cell line inhibiting cell proliferation and trigger apoptosis in effect. Three-dimensional tumor spheroids in the body structure of the model to better reflect the results we obtained shed light on the treatment of hepatocellular carcinoma think

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