Halil AŞCI, Mehtap SAVRAN, Nurhan GÜMRAL, Selçuk ÇÖMLEKÇİ, Özlem ÖZMEN

RATLARDA 2.45 GHz ELEKTROMANYETİK ALAN KAYNAKLI KALP DOKUSU HASARINDA C VİTAMİNİNİN KORUYUCU ETKİLERİ

AmaçBir elektromanyetik alan (EMA) dalga formu olan radyofrekans (RF) dalga aralığında bulunan 2.45 GHzelektromanyetik radyasyonun (EMR) endüstriyel, askeri ve bilimsel alanlarda kullanımı yaygındır. Bu çalışmada, EMA’nın kalp dokusu üzerine olan zararlı etkileri ve C vitamininin (C vit) bu hasara karşı koruyucuolup olmadığı değerlendirilmiştir.Gereç ve YöntemEMA’nın kalp dokusu üzerine etkisi ve C vit’in olasıkoruyucu rolünü değerlendirmek için 18 dişi sıçankontrol, EMA ve EMA + C vit olarak gruplara ayrıldı.Sıçanların hareketini kısıtlayan bir düzenek yardımıyla EMA ve EMA + C vit gruplarına 30 gün süreyle,günde 60 dakika 2.24 mW/kg gücündeki 2.45 GHzEMA maruziyeti sağlandı. Tedavi grubuna ayrıca 30gün boyunca C vit uygulandı.BulgularDeney sonunda kalp dokularında yapılan analizlerde, EMA grubunun total antioksidan kapasite (TAS)değerlerinde kontrol grubuna göre sayısal bir azalma gözlenirken, EMA + C vit grubundaki TAS değerlerinde hem kontrol hem de EMA grubuna göre istatistiksel olarak anlamlı bir artış gözlendi (p: 0.036ve p: 0.015; sırasıyla). EMA grubunda total oksidankapasite (TOS) ve oksidatif stres indeksi (OSİ) kontrol grubuna göre istatistiksel olarak anlamlı şekildeyüksek iken (p: 0.004 ve p: 0.005; sırasıyla), EMA+C vit grubunda TOS değerindeki azalma istatistikselolarak anlamlı bulunmadı. Histopatolojik olarak, EMAgrubunda belirgin hiperemi dışında major bir anomaligözlenmedi. EMA grubunda kaspaz 3 ve kaspaz 8 aktivitelerinde anlamlı bir artış izlenirken (p

PROTECTIVE EFFECTS OF VITAMIN C ON 2.45 GHz ELECTROMAGNETIC FIELD-INDUCED HEART TISSUE DAMAGE IN RATS

Objective The use of 2.45 GHz electromagnetic radiation (EMR) in the radiofrequency (RF) wave-range, that is a wave form of an electromagnetic fields (EMF), is widely used in industrial, military and scientific fields. In this study, the harmful effects of EMF on heart tissue and whether Vitamin C (C vit) is protective aganist this damage were evaluated. Material and Methods 18 female rats were divided into control, EMF and EMF + C vit groups to evaluate the effect of EMF on heart tissue and the possible protective role of C vit. EMF and EMF + C vit groups were exposed to 2.45 GHz EMF at a power of 2.24 mW / kg for 30 days for a period of 60 minutes/ day with the aid of a mechanism that restricted the movement of the rats. C vit treatment was also applied to the treatment group for 30 days. Results At the end of the experiment, the total antioxidant status (TAS) values of the EMF group showed a numerical decrease compared to the control group, while the TAS values in the EMF + C vit group showed a statistically significant increase compared to both the control and EMF groups (p: 0.036 and p: 0.015; respectively). While total oxidant status (TOS) and oxidative stress index (OSI) were significantly higher in the EMF group than in the control group (p: 0.004 and p: 0.005; respectively), the decrease in TOS value in the EMA + C vit group was not statistically significant. Histopathologically, there was no major abnormality except for marked hyperemia in the EMF group. It was observed that caspase-3 and caspase-8 activities increased (p

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