Fethiye Çağlar ÖZŞAHİN, ZUHAL YETKİN AY, Duygu Kumbul DOĞUÇ, Birsen Harun DAĞDEVİREN, Ersin USKUN, FATMA YEŞİM KIRZIOĞLU

The Effect of the Overweight on the Circulating Cytokines and Adipokines in Experimental Periodontitis

Amaç: İnterlökin (IL)-1?, IL-4, IL-6 ve tümör nekrozis faktör (TNF)-? periodontal patogenezde anahtar sitokinlerdir. Bu çalışma periodontitisi olan fazla kilolu ratlarda serumda daha yüksek düzeyde pro-inflamatuvar sitokin ve/veya adipokin ve daha düşük düzeyde anti-inflamatuvar sitokin ve/veya adipokin düzeyi olduğu hipotezini test etmeyi amaçlamaktadır. Materyal-Metot: Dört aylık 32 erkek Wistar rat normal ağırlıklı ve periodontal sağlıklı (n=8), normal ağırlıklı periodontitisli (n=8), fazla kilolu periodontitisli (n=8) ve fazla kilolu periodontal sağlıklı (n=8) olmak üzere dört gruba ayrıldı. Periodontitis ligatürle 14 günde oluşturuldu. Serum sitokin ve adipokin düzeyleri ELISA ile incelendi. Bulgular: Normal ağırlıklı periodontitisli olan ve olmayan ratlar fazla kilolu olan eş gruplarından daha yüksek IL-4 düzeyi sergiledi (P 0,01). Sonuç: Bu deneysel modelde fazla kilolu olma durumu serum sitokin ve adipokin düzeylerini periodontitisten bağımsız olarak etkilemiştir. Fazla kilolu olan periodontitis olan ve olmayan ratların anti-inflamatuvar sitokin ve adipokinler (IL-4 ve adiponektin) düşük, pro-inflamatuvar bir sitokin olan IL-6 ise normal ağırlıklı eşlerinden daha yüksek olarak bulunmuştur.

Deneysel Periodontitiste Fazla Kilolu Olma Durumunun Dolaşımdaki Sitokinler ve Adipokinler Üzerine Etkisi

Objective: Interleukin (IL)-1?, IL-4, IL-6, and tumor necrosis factor (TNF-?) are forefront cytokines in the periodontitis pathogenesis. Therefore, this study aimed to test the hypothesis that overweight rats with periodontitis have higher pro-inflammatory cytokines and/or adipokines, and lower anti-inflammatory cytokines and/or adipokines in serum than normal weight rats. Material-Method: Thirty-two 4-month old male Wistar rats were divided into 4 groups: normal weight, periodontally healthy rats (NH, n = 8), normal weight rats with periodontitis (NP, n = 8), overweight, periodontally healthy rats (OH, n = 8); and overweight rats with periodontitis (OP, n = 8). Periodontitis was induced by ligature for 14 days. The serum cytokine' and adipokine' levels were investigated using ELISA. Results: IL-4 levels were higher in the NH and NP groups than their counterparts in the overweight rats (P <0.01). The IL-6 level was significantly higher in the OP group than in the NP group (P < 0.01). The adiponectin level was significantly higher in the NH group than in the OH and OP groups (P < 0.01). Leptin and resistin levels didn't differ significantly among the groups (P > 0.01). Conclusions: The overweight status affected the cytokine' and adipokine' level independent from the periodontal status in this experimental model. Overweight rats with and without periodontitis have lower serum levels of anti-inflammatory cytokine and adipokines (IL-4 and adiponectin) and higher serum levels of pro-inflammatory cytokine (IL-6) than their counterparts in normal weight rat groups.

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