Reduced Graphene Oxide/α-Cyclodextrin-Based Electrochemical Sensor: Characterization and Simultaneous Detection of Adenine, Guanine and Thymine

Graphene, the rising star of carbon nanomaterials, is a single layer of sp2-bonded carbon atoms patterned in a 2D honeycomb network. Thanks to its unique features, graphene has attracted enormous attention and it has arisen various applications in the fields of optical and electrochemical sensors. In the present work, reduced graphene oxide/alpha cyclodextrin (rGO/α-CD) is proposed as a nanocomposite for individual and simultaneous detection of adenine, guanine and thymine. rGO/α-CD has been characterized by FT-IR, Raman spectroscopy, AFM, HR-TEM and SEM techniques. Cyclic voltammetry, differential pulse voltammetry and chronoamperometry techniques were utilized for detection of adenine, guanine and thymine. The limit of detection (LOD) values for adenine, guanine and thymine were calculated to be 145.5, 38.9 and 52.9 nmol L-1, respectively. The results show that the developed sensor can be utilized for the determination of adenine, guanine and thymine in human serum, indicating its promising application in the analysis of real samples.

Anahtar Kelimeler:

Graphene Oxide, Cyclodextrin; Electrochemical Sensor; Adenine; Guanine; Thymine

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[1] Ferrari, A. C., Bonaccorso, F., Fal’ko, V., Novoselov, K. S., Roche, S., Boggild, P., Borini, S., Koppens, F. H. L., Palermo, V., Pugno, N., Garrido, J. A., Sordan, R., Bianco, A., Ballerini, L., Prato, M., Lidorikis, E., Kivioja, J., Marinelli, C., Ryhanen, T., Morpurgo, A., Coleman, J. N., Nicolosi, V., Colombo, L., Fert, A., Garcia-Hernandez, M., Bachtold, A., Schneider, G. F., Guinea, F., Dekker, C., Barbone, M., Sun, Z., Galiotis, C., Grigorenko, A. N., Konstantatos, G., Kis, A., Katsnelson, M., Vandersypen, L., Loiseau, A., Morandi, V., Neumaier, D., Treossi, E., Pellegrini, V., Polini, M., Tredicucci, A., Williams, G. M., Hee Hong, B., Ahn, J.-H., Min Kim, J., Zirath, H., van Wees, B. J., van der Zant, H., Occhipinti, L., Di Matteo, A., Kinloch, I. A., Seyller, T., Quesnel, E., Feng, X., Teo, K., Rupesinghe, N., Hakonen, P., Neil, S. R. T., Tannock, Q., Lofwander, T., Kinaret, J. 2015. Science and Technology Roadmap for Graphene, Related Two-Dimensional Crystals, and Hybrid Systems. Nanoscale, 7(11), 4598–4810.
[2] Geim, A. K., Novoselov, K. S. 2007. The Rise of Graphene. Nature Materials, 6(3), 183–191.
[3] Novoselov, K. S., Fal′ko, V. I., Colombo, L., Gellert, P. R., Schwab, M. G., Kim, K. 2012. A Roadmap for Graphene. Nature, 490(7419), 192–200.
[4] Geim, A. K. 2011. Random Walk to Graphene (Nobel Lecture). Angewandte Chemie International Edition, 50(31), 6966–6985.
[5] Guo, Y., Guo, S., Ren, J., Zhai, Y., Dong, S., Wang, E. 2010. Cyclodextrin Functionalized Graphene Nanosheets with High Supramolecular Recognition Capability: Synthesis and Host - Guest Inclusion for Enhanced Electrochemical Performance. ACS Nano, 4, 4001–4010.
[6] Tan, L., Zhou, K.-G., Zhang, Y.-H., Wang, H.-X., Wang, X.-D., Guo, Y.-F., Zhang, H.-L. 2010. Nanomolar Detection of Dopamine in The Presence of Ascorbic Acid At Β-Cyclodextrin/Graphene Nanocomposite Platform. Electrochemistry Communications, 12(4), 557–560.
[7] Ambrosi, A., Chua, C. K., Bonanni, A., Pumera, M. 2014. Electrochemistry of Graphene And Related Materials. Chemical Reviews, 114(14), 7150–7188.
[8] Zor, E., Bingol, H., Ramanaviciene, A., Ramanavicius, A., Ersoz, M. 2015. An Electrochemical and Computational Study for Discrimination of D- And L-Cystine by Reduced Graphene Oxide/Beta-Cyclodextrin. Analyst, 140(1), 313–321.
[9] Shahgaldian, P., Pieles, U. 2006. Cyclodextrin Derivatives as Chiral Supramolecular Receptors for Enantioselective Sensing. Sensors, 6, 593–615.
[10] Feng, W. L., Liu, C., Lu, S. Y., Zhang, C. Y., Zhu, X. H., Liang, Y., Nan, J. M. 2014. Electrochemical Chiral Recognition of Tryptophan Using A Glassy Carbon Electrode Modified with Beta-Cyclodextrin and Graphene. Microchimica Acta, 181(5-6), 501–509.
[11] Zor, E., Esad Saglam, M., Alpaydin, S., Bingol, H. 2014. A Reduced Graphene Oxide/α-Cyclodextrin Hybrid for the Detection of Methionine: Electrochemical, Fluorometric and Computational Studies. Analytical Methods, 6(16), 6522-6530.
[12] Alarcón-Angeles, G., Pérez-López, B., Palomar-Pardave, M., Ramírez-Silva, M. T., Alegret, S., Merkoçi, A. 2008. Enhanced Host–guest Electrochemical Recognition of Dopamine Using Cyclodextrin in the Presence of Carbon Nanotubes. Carbon, 46(6), 898–906.
[13] Hui, Y., Ma, X., Hou, X., Chen, F., Yu, J. 2015. Silver Nanoparticles-β-Cyclodextrin-Graphene Nanocomposites Based Biosensor for Guanine and Adenine Sensing. Ionics, 21(6), 1751–1759.
[14] Zhang, M., Zhao, H. T., Yang, X., Dong, A. J., Zhang, H., Wang, J., Liu, G. Y., Zhai, X. C. 2016. A Simple and Sensitive Electrochemical Sensor for New Neonicotinoid Insecticide Paichongding in Grain Samples Based on β-Cyclodextrin-Graphene Modified Glassy Carbon Electrode. Sensors and Actuators B Chemical, 229, 190–199.
[15] Jiang, Z., Li, G., Zhang, M. 2016. Electrochemical Sensor Based on Electro-Polymerization of β-Cyclodextrin and Reduced-Graphene Oxide on Glassy Carbon Electrode for Determination of Gatifloxacin. Sensors and Actuators B Chemical, 228, 59–65.
[16] Zhan, F., Gao, F., Wang, X., Xie, L., Gao, F., Wang, Q. 2016. Determination of lead(II) by Adsorptive Stripping Voltammetry Using a Glassy Carbon Electrode Modified with β-Cyclodextrin and Chemically Reduced Graphene Oxide Composite. Microchimica Acta, 183, 1169–1176.
[17] Anu Prathap, M. U., Srivastava, R., Satpati, B. 2013. Simultaneous Detection of Guanine, Adenine, Thymine, and Cytosine at polyaniline/MnO2 Modified Electrode. Electrochimica Acta, 114, 285–295.
[18] Erdem, A. 2007. Nanomaterial-Based Electrochemical DNA Sensing Strategies. Talanta, 74, 318–325.
[19] Li, J., Kuang, D., Feng, Y., Zhang, F., Xu, Z., Liu, M., Wang, D. 2013. Electrochemical Tyrosine Sensor Based on a Glassy Carbon Electrode Modified with a Nanohybrid Made from Graphene Oxide and Multiwalled Carbon Nanotubes. Microchimica Acta, 180(1), 49–58.
[20] Ferrari, A. C., Robertson, J. 2000. Interpretation of Raman Spectra of Disordered and Amorphous Carbon. Physical Review B, 61(20), 14095–14107.
[21] Graf, D., Molitor, F., Ensslin, K., Stampfer, C., Jungen, A., Hierold, C., Wirtz, L. 2007. Spatially Resolved Raman Spectroscopy of Single- and Few-Layer Graphene. Nano Letters, 7(2), 238–242.
[22] Peng, L., Xu, Z., Liu, Z., Wei, Y., Sun, H., Li, Z., Zhao, X., Gao, C. 2015. An Iron-Based Green Approach to 1-H Production of Single-Layer Graphene Oxide. Nature Communucations, 6(5716), 1-9.
[23] Park, S., Ruoff, R. S. 2009. Chemical Methods for the Production of Graphenes. Nature Nanotechnology, 4(4), 217–224.
[24] Shrivastava A, Gupta VB. 2011. Methods for the determination of limit of detection and limit of quantitation of the analytical methods. Chronicles of Young Scientists, 2(1), 21-25.
[25] Miller, J. N.; Miller, J. C. 2005. Statistics and Chemometrics for Analytical Chemistry. 5th edition. Pearson Education/Prentice-Hall, Harlow, 285s.
[26] Thangaraj, R., Senthil Kumar, A. 2013. Simultaneous Detection of Guanine and Adenine in DNA and Meat Samples Using Graphitized Mesoporous Carbon Modified Electrode. Journal of Solid State Electrochemistry, 17(3), 583–590.
[27] Chen, J., Chen, S., Weng, W., Li, Y., Yeh, S. 2013. Simultaneous Detection of DNA Bases on Electrodes Chemically Modified with Graphene - New Fuchsin. International Journal of Electrochemical Science, 8, 3963–3973.