Nano-elektrokimyasal Biyosensörler Kullanılarak DNA ile Doksorubisin Etkileşiminin Araştırılması

Bu çalışmada, nano-elektrokimyasal biyosensörler kullanılarak çift sarmallı deoksiribonükleik asit (dsDNA) ve doksorubisin (DOX) arasındaki etkileşim diferansiyel puls voltametrisi yöntemiyle araştırılmıştır. Biyosensör yüzeyi setil trimetilamonyum (sab) ve karbon nanotüp (knt) içeren çözeltiden elektrodepozisyon yöntemiyle poli(sab)-knt sentezi ile hazırlanmıştır. DNA-ilaç etkileşimi araştırmak için indikatör olarak dsDNA elektroaktif bazların voltametrik sinyalleri kullanılmıştır. İlaç-DNA etkileşimi sonrası hem guanin hem de adenin bazlarının oksidasyon pik akımlarının azaldığı gözlenmiştir. İlacın bağlanma süresi ve derişiminin dsDNA bazlarının voltametrik sinyalleri üzerindeki etkisi de değerlendirilmiştir. DOX için doğrusal çalışma aralığı 0,39-25 µg mL-1 arasında ve gözlenebilme sınırı 0,26 µg mL-1 olarak bulunmuştur. Elektrokimyasal ve spektrokimyasal çalışmalar, DOX ve dsDNA arasındaki etkileşim mekanizmasının interkalasyon modu ile gerçekleştiğini göstermiştir.

Anahtar Kelimeler:

Biyosensör, Voltametri, Elektrokimya, DNA-ilaç etkileşimi

Investigation of Doxorubusin Interactions with DNA by Using Nano-electrochemical Biosensors

In this study, the interaction between double-stranded deoxyribonucleic acid (dsDNA) and doxorubicin (DOX) was investigated using nano-electrochemical biosensors by differential pulse voltammetry. The biosensor surface was prepared from a solution containing cetyl trimethylammonium (cab) and carbon nanotubes (cnt) with the synthesis of poly(sab)-knt by electrodeposition method. Voltammetric signals of dsDNA electroactive bases were used as indicators to investigate DNA-drug interactions. It was observed that the oxidation peak currents of both guanine and adenine bases decreased after drug-DNA interaction. The linear range for DOX was found to be between 0.39-25 μg mL-1 and limit of detection was found as 0.26 μg mL-1. Besides, the drug-DNA interaction was demonstrated spectrochemically by UV-vis spectroscopy. Electrochemical and spectrochemical studies have shown that the interaction mechanism between DOX and dsDNA occurs with the intercalation mode.

Keywords:

Biosensor, Voltammetry, Electrochemistry, DNA-drug interaction,

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