Mentha longifolia (L.) ssp. longifolia Essential Oil: Source of Natural Antioxidant and Antimutagen as Food Additive

This research was performed to control the antioxidant activity, mutagenicity and antimutagenic effect of Mentha longifolia (L.) ssp. longifolia essential oil (EO), which is considered as a possible ingredient when producing healthy food. The antiradical activity was established using DPPH (2,2-diphenyl-1-picrylhydrazyl radical) and β-carotene/linoleic acid bleaching assays. The total phenolic content in the EO was evaluated by Folin Ciocalteau method (FCR). Ames Salmonella/microsome mutagenicity assay was applied todetect possible mutagenic and antimutagenic behavior. Our observations reveal that the IC50 value for DPPH radicals was 5.27 ± 0.13 mg/mL. The total antioxidant efficiency increased with an increase in the concentration of the EO, and IC50 value 11.7 ± 0.21 mg/mL. The total of phenolics was 186 ± 8.9 mg/g gallic acid equivalent/EO. Also, any concentrations of the EO used did not show mutagenic action but exhibited strong antimutagenic effects at 10.0-4.0 µg/plate concentrations. This research proposes that because of the antioxidant and antimutagenic characteristics, the EO is very advantageous and significant to the company’s manufacturing food additives.

Anahtar Kelimeler:

Antimutagenic, Antioxidant; Essential oil; Food ingredient; Mentha longifolia

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[1] Alamed, J., Chaiyasit, W., McClements D. J., Decker E. A. 2009. Relationships between free radical scavenging and antioxidant activity in foods. Journal of Agricultural and Food Chemistry, 8, 2969-2976.
[2] Kubow S. 1990. Toxicity of dietary lipid peroxidation products. Trends in Food Science & Technology, 1,67-71.
[3] Lobo, V., Pati, A., Phatak, A., Chandra, N. 2010. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacognosy Reviews, 4(8), 118-126.
[4] Wanasundra, P. K. J. P. D., Shahidi, F. 2005. Antioxidants: Science, technology, and applications. In F. Shahidi editor, Bailey’s industrial oil, fat products 6th edition, N.J. Hoboken, John Wiley and Sons Inc. 431-489s.
[5] Shahidi, F., Ambigaipalan, P. 2015. Phenolics and polyphenolics in foods, beverages and spices: Antioxidant activity and health effects – A review. Journal of Functional Foods, 18, 820-897.
[6] Padmashree, A., Roopa, N., Semwal, A.D., Sharma, G.K., Agathian, G., Bawa, A.S. 2007. Star-anise (Illicium verum) and black caraway (Carum nigrum) as natural antioxidants. Food Chemistry, 104 (1), 59-66.
[7] Negi, P.S. 2012. Plant extracts for the control of bacterial growth: Efficacy, stability and safety issues for food application. International Journal of Food Microbiology, 156, 7-17.
[8] Bajpai, V.K., Baek, K.H., Kang, S.C. 2012. Control of Salmonella in foods by using essential oils: a review. Food Research International, 45, 722-734.
[9] Teixeira, B., Marques, A., Ramos, C., Batista, I., Serrano, C., Matos, O., Neng, N.R., Nogueira, J.M.F., Saraiva, J.A., Nunes, M.L. 2012. European pennyroyal (Mentha pulegium) from Portugal: chemical composition of essential oil and antioxidant and antimicrobial properties of extracts and essential oil. Industrial Crops and Products, 36, 81-87.
[10] Llana-Ruiz-Cabello, M., Pichardo, S., Maisanaba, S., Puerto, M., Prieto, A.I., Gutiérrez-Praena, D., Jos, A., Cameán, A.M.2015. In vitro toxicological evaluation of essential oils and their main compounds used in active food packaging: A review. Food and Chemical Toxicology, 81, 9-27.
[11] Prakash, B., Kiran, S. 2016. Essential oils: a traditionally realized natural resource for food. Current Science, 110(10), 1890-1892.
[12] Gulluce, M., Sahin, F., Sokmen, M., Ozer, H., Daferera, D., Sokmen, A., Polissiou, M., Adiguzel, A., Ozkan, H.2007. Antimicrobial and antioxidant properties of the essential oils and methanol extract from Menthalongifolia L. ssp. longifolia. Food Chemistry, 103, 1449-1456.
[13] Mkaddem, M., Bouajila, J., Ennajar, M., Lebrihi, A., Mathieu, F. Romdhane, M. 2009. Chemical composition and antimicrobial and antioxidant activities of Mentha (longifolia L. and viridis) essential oils. Journal of Food Science, 74(7), 358-363.
[14] Sarac, N., Ugur, A. 2009. The in vitro antimicrobial activities of the essential oils of some Lamiaceae species from Turkey. Journal of Medicinal Food, 12(4), 902-907.
[15] Barros, A de S., Morais, S.M., Ferreiraa, P.A.T., Vieira, Í.P.G., Craveiroc, A.A., Fontenelle, R.O.S., de Menezes, J.E.S.A., da Silva, F.W.F.de Sousa,H. A. 2015. Chemical composition and functional properties of essential oils from Mentha species. Industrial Crops and Products, 76, 557-564.
[16] Alsarar, A.S., Hussein, H.I., Abobakr, Y., Bayoumi, A. E., Alotaibi, M. T. 2014. Fumigant toxicity and antiacetylcholinesterase activity of Saudi Mentha longifolia and Lavandula dentata species against Callosobruchus maculatus (F.) (Coleoptera: Bruchidae). Turkish Journal of Entomology. 38, 11-18.
[17] Jalilzadeh-Amin G., Maham M. 2015. Antidiarrheal activity and acute oral toxicity of Mentha longifolia L. essential oil. Avicenna Journal of Phytomedicine, 5(2), 128-1237.
[18] Ebrahimabadi, A.H., Mazoochi, A., Kashi, F.J., Djafari-Bidgoli, Z., Batooli, H. 2010. Essential oil composition and antioxidant and antimicrobial properties of the aerial parts of Salvia eremophilaBoiss.from Iran. Food and Chemical Toxicology, 48(5), 1371-1376.
[19] Rauter, A.P., Dias, C., Martins, A., Branco, I., Neng, N.R., Nogueira, J. M., Goulart, M., Silva, F.V.M., Justino, J., Trevitt, C., Waltho, J.P. 2012. Non-toxic Salvia sclareoides Brot. extracts as a source of functional food ingredients: Phenolic proﬁle, antioxidant activity and prion binding properties. Food Chemistry, 132, 1930-1935.
[20] Singleton, V.L., Orthofer, R., Lamuel-Raventos, R.M., 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology, 299, 152-178.
[21] Mortelmans, K., Zeiger, E. 2000. The Ames Salmonella/microsome mutagenicity assay. Mutation Research, 455, 29-60.
[22] Maron, D., Ames, B. N. 1983. Revised methods for the Salmonella mutagenicity test. Mutation Research, 113, 173-215.
[23] Taghvaei, M., Jafari, S. M. 2015. Application and stability of natural antioxidants in edible oils in order to substitute synthetic additives. Journal of Food Science and Technology, 52(3), 1272-1282.
[24] Shahidi F., 1998. Indicators for evaluation of lipid oxidation and off-flavor development in food. Elsevier Amsterdam, The Netherlands, 55s.
[25] Chahmi, N., Anissi, J., Jennan, S., Farah, A.,Sendide, K. El Hassoun, M., 2015. Antioxidant activities and total phenol content of Inula viscose extracts selected from three regions of Morocco. Asian Pacific Journal of Tropical Biomedicine, 5(3), 228-233.
[26] Ramarathnam, N., Osawa, T., Namiki, M. Tashiro, T. 1986. Studies on the relationship between antioxidative activity of rice hull and germination ability of rice seeds. Journal of the Science of Food and Agriculture, 37, 719-726.
[27] Al-Ali, K., Abdelrazik, M., Alghaithy, A., Diab, A., El-Beshbishy, H., Baghdadi H. 2014. Antimutagenic and anticancer activity of Al Madinah Alhasawy mint (Mentha longifolia) leaves extract. Pakistan Journal of Biological Sciences, 17, 1231-1236.
[28] Baris O., Karadayi, M., Yanmis, D., Guvenalp, Z., Bal, T., Gulluce, M., 2011. Isolation of 3 flavonoids from Mentha longifolia (L.) Hudson subsp. Longifolia and determination of their genotoxic potentials by using the E. coli WP2 test system. Journal of Food Science, 76(9), 212-217.
[29] Gulluce, M., Orhan, F., Adiguzel, A., Bal, T., Guvenalp, Z. Dermirezer, L. O. 2013. Determination of antimutagenic properties of apigenin-7-O-rutinoside, a flavonoid isolated from Mentha longifolia (L.) Huds. ssp. longifolia withyeast DEL assay. Toxicology and Industrial Health, 29(6), 534-540.
[30] Ghahramanifar, M., Mirzaei, M., Mirzaei, A. Azemi, M. 2013. Cytotoxicity and antimutagenicity screening of 5 Iranian medicinal plants using Artemia urmiana and Ames Test. International Journal of Biology, Pharmacy and Allied Sciences, 2(3), 654-664.
[31] Orhan, F., Barış, Ö., Yanmış, D., Bal, T., Güvenalp, Z., Güllüce, M. 2012. Isolation of some luteolin derivatives from Menthalongifolia (L.)Hudson subsp. longifolia and determination of their genotoxic potencies. Food Chemistry, 135(2), 764-769.