Roberto PILLOTON, Suna TIMUR, Dilek ODACI DEMIRKOL, Caglar OZDEMIR

Karbon Nanotüple Modifiye Edilmiş, Şablonla Basılmış Elektrotlar: Amperometrik Enzim Sensörleri için Piranoz Oksidaz İmmobilizasyon Platformu

Burada, çok duvarlı karbon nanotüp ile modifiye edilmiş şablonla baskılanmış elektrotları (MWCNT‐SPE) kullanarak yeni bir enzimatik biyosensör geliştirilmiştir. Piranoz oksidaz (PyOx) jelatin membran vasıtasıyla elektrotların yüzeyine immobilize edilmiştir ve gluraldehid kullanarak çapraz bağlanma yapılmıştır. Glukoz; enzimatik reaksiyon sırasında kullanılan oksijen takip edilerek ‐0,7 V'da (Ag/AgCl'e karşı) belirlenmiştir. pH ve enzim miktarının optimizasyonundan sonra, glukoz tayini için doğrusal aralık 0,1‐1,0 mM aralığı olarak bulunmuştur. Daha sonra, karbon nanotüplerin biyosensör cevabına etkisi test edilmiştir. Aynı zamanda piranoz oksidaz yerine glukoz oksidaz içeren enzimatik biyosensör hazırlanmıştır ve glukoz için biyosensör cevabı takip edilmiştir. Bunların dışında, içeceklerde glukoz analizi için sistem uygulanmıştır.

Carbon Nanotube Modified Screen Printed Electrodes: Pyranose Oxidase Immobilization Platform for Amperometric Enzyme Sensors

Here, a novel enzymatic biosensor was developed using multiwalled carbon nanotube including screen printed electrodes (MWCNT‐SPE). Pyranose oxidase (PyOx) was immobilized on the electrode surface by way of gelatin membrane and then cross‐linked using glutaraldehyde. Glucose was detected at ‐ 0.7 V (vs. Ag/AgCl) by watching consumed oxygen in enzymatic reaction after addition substrate. After optimization of pH and enzyme loading, the linearity was found in the range of 0.1–1.0 mM of glucose. After that, the effect of MCNT on the current was tested. Also the enzymatic biosensor including glucose oxidase instead of pyranose oxidase was prepared and the biosensor response followed for glucose. Furthermore, this system was tested for glucose analysis in soft drinks.

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