β-D-Glukan, Lektin, Linoleik Asit ve β-Karoten Bileşiklerinin Karbonik Anhidraz Enzimleri Üzerindeki Etkilerinin Araştırılması

β-D-glukan, lektin, linoleik asit ve β-karoten; hayvansal, bitkisel ve bakteriyel kaynaklarda bulunan, biyolojik olarak aktif bileşiklerdir. Glokom, dünya genelinde körlüğe neden olma bakımından katarakttan sonra ikinci sırada gelen bir hastalıktır. Karbonik anhidraz (CA) inhibitörleri uzun yıllardır glokom tedavisinde kullanılmaktadır. Ancak, inhibitör ajanların yan etkileri azımsanmayacak derecede çoktur. Yeni CA inhibitörlerinin geliştirilmesi üzerine çeşitli araştırma grupları çalışmalar yapmaktadırlar. Doğal kaynaklı bileşiklerin CA inhibitörü olarak kullanımının araştırılması da son yıllarda popülerlik kazanmıştır. Bu çalışmada, β-D-glukan, lektin, linoleik asit ve β-karoten bileşiklerinin, insan eritrosit CA izoenzimleri olan hCA I ve hCA II üzerine etkileri in vitro koşullarda araştırılmıştır. Bileşiklerin Ki değerleri hCA I için 0.45±0.09 µM − 37.02±17.85 µM aralığında, hCA II için 3.12±1.38 µM − 61.23±25.46 µM aralığındadır.

Anahtar Kelimeler:

β-D-glukan, Lektin, Linoleik asit, β-karoten, Karbonik anhidraz

Investigation of the Effects of Compounds, β-D-Glucan, Lectin, Linoleic Acid and β-Carotene on Carbonic Anhydrase Enzymes

β-D-glucan, lectin, linoleic acid and β-carotene are biologically active compounds and they are found in animal, herbal and bacterial sources. Glaucoma is the second most common cause of blindness worldwide after cataract. Carbonic anhydrase (CA) inhibitors have been used in the treatment of glaucoma for years. However, inhibitory agents have a lot of side effects. Research groups have been working on the development of new CA inhibitors. Research into the use of compounds of natural origin as CA inhibitors has also gained popularity in recent years. In this study, the inhibitory effects of β-D-glucan, lectin, linoleic acid and β-carotene on human erythrocyte carbonic anhydrase isoenzymes, hCA I and hCA II, have been studied in vitro. The Ki values of the compounds were in the range of 0.45±0.09 µM − 37.02±17.85 µM for hCA I, and 3.12±1.38 µM − 61.23±25.46 µM for hCA II.

Keywords:

β-D-glucan, Lectin, Linoleic acid, β-carotene, Carbonic anhydrase,

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