Perihan AKBAŞ, Hamit USLU, Gözde ATİLA USLU, Hicran ALKAN

Hyoscyamus reticulatus L. Tohum Ekstraktının Antimikrobiyal ve Apoptotik Etkinliğinin Araştırılması

Hyoscyamus reticulatus Solanacea ailesindendir ve ülkemizde altı türü bulunmaktadır. Hyoscyamus türleri özellikle halk arasında yaygın olarak kullanılan skopolamin ve hiyosiyamin gibi tropan alkaloitleri kaynağıdır. H. reticulatus tohumundan soxhlet yöntemi ile elde edilen etil alkol ekstresinin bazı Gram (-) ve Gram (+) bakteri ve mayalara karşı antimikrobiyal aktivitesinin tespit edilmesi ve H. reticulatus tohumunun plazma ve beyin numunelerinde TP53 ve Bcl-2 seviyeleri ölçülerek apoptotik etkisinin belirlenmesi amaçlanmıştır. Çalışmada 40 adet Wistar albino cinsi dişi sıçan her grupta 10 adet olacak şekilde 4 gruba ayrıldı; Kontrol (K), 125, 250, 500 mg/kg H. reticulatus ekstrakt grupları. H. reticulatus tohumu ekstraktının Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae, Pasteurella multicoda, Pseudomonas aeruginosa, Salmonella enteridis, Staphylococcus aureus, Yersinia enterocolitica bakterileri ve Candida albicans mayasına karşı antimikrobiyal etkinliğinin bulunmadığı tespit edilmiştir. HR250 ve HR500 gruplarının plazma örneklerinde TP53 seviyelerinin K grubuna göre önemli düzeyde arttığı tespit edildi (sırasıyla p<0,01, p<0,001). Ekstrakt uygulanan tüm gruplarla K grubu karşılaştırıldığında; ekstrakt uygulanan gruplarda plazma Bcl-2 seviyelerinin önemli düzeyde azaldığı belirlendi (p<0,001). Sonuç olarak; H. reticulatus tohum ektraktının antimikrobiyal etkisi olmadığı ve in vivo olarak farklı dozlarda uygulanan ekstraktın beyin dokusunda olmasa da diğer organ ve/veya organlarda apoptozisi uyarabileceğini düşünmekteyiz.

Anahtar Kelimeler:

Antimikrobiyal, Bcl-2, Hyoscyamus reticulatus tohum ekstraktı, TP53

Investıgatıon of the Antimicrobial and Apoptotic Efficacy of Hyoscyamus retıculatus L. Seed Extract

Hyoscyamus reticulatus is from Solanacea famil and has six species in our country. Hyoscyamus species are the source of tropan alkaloids, such as scopolamine and hyoctiamine, which are commonly used in public. The aim of this study was to determine the antimicrobial activity of ethyl alcohol extract obtained from H. reticulatus seed by soxhlet method against some Gram (+) and Gram (-) bacteria and yeasts and to determine apoptotic effect of H. reticulatus seed by measuring TP53 and Bcl-2 levels in plasma and brain samples. 40 Wistar albino female rats were divided into 4 groups in each group with 10 of them; Control (C), 125, 250, 500 mg / kg H. reticulatus extract groups. It was found that H. reticulatus seed extract have no antimicrobial activity against Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae, Pasteurella multicoda, Pseudomonas aeruginosa, Salmonella enteridis, Staphylococcus aureus, Yersinia enterocolitica bacteria and Candida albicans yeast. Plasma samples of HR250 and HR500 groups showed significant increase in TP53 levels compared to group C (p <0.01, p <0.001, respectively). Compared to all extract applied groups and group C; Plasma Bcl-2 levels significantly decreased in extract groups (p <0.001). As a result; we suggest that H. reticulatus seed extract does not have an antimicrobial effect and extracts applied in different doses in vivo may stimulate apoptosis in other organ and / or organs, although not in brain tissue.

Keywords:

Antimicrobial, Bcl-2, Hyoscyamus reticulatus seed extract, TP53,

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