Aptamer konjuge edilmiş yarı iletken nanoparçacıkları temel alan patojen bir mikroorganizmanın elektrokimyasal belirlenmesi ve floresans görüntülenmesi
Aptamerler, küçük moleküllerden memeli hücrelerine kadar çeşitli boyutlardaki hedeflere karşı seçici moleküllerdir. Burada, E. coli O157:H7 dedeksiyonu için QD içeren elektrokimyasal aptasensör rapor edilmiştir. Yüzeyler, amino ve tiyol grupları içeren sisteamin (CysN) ile kendiliğinden oluşan tek tabaka meydana gelişi ile modifiye edildi. Karboksil ile fonksiyonelleştirilen quantum noktalar (QD) ve aptamerler (Apt), sisteaminle modifiye edilmiş altın elektrotlara konjuge edildi. CysN/QD/Apt modifiye edilen Au yüzeylerde inkübasyon sorasında, E. coli O157:H7 için doğrusal aralık 1 to 102 CFU/mL idi. QD floresans yüzeyler sağlar, böylece hücrelerin adezyonu floresans mikroskop kullanılarak takip edildi. Adezyon sonrası hücreler aynı zamanda taramalı electron mikroskobu ile görüntülendi. Son olarak, hücre analizi gerçek örneklerde gerçekleştirildi.
Electrochemical sensing and fluorescence imaging of E. coli O157:H7 based on aptamerconjugated semiconducting nanoparticles
Aptamers are selective molecules against to various sizes of targets fromsmall molecules to mammalian cells. Here, we reported QDs containingelectrochemical aptasensor for the detection of E. coli O157:H7. The electrodesurfaces were modified by cysteamine (CysN), which has amine and thiol groups,via self-assembled monolayer formation. The carboxyl-functionalized quantumdots (QD) and aptamers (Apt) were conjugated to cysteamine modified goldelectrodes. Linear range for E. coli O157:H7 was from 1 to 102 CFU/mL afterincubation on CysN/QD/Apt modified Au surfaces. QDs provide fluorescencesurface, so that adhesion of cells was followed using fluorescence microscope.Adhered cells were also imaged by scanning electron microscopy. Finally, cellanalysis was carried out in real samples.
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