Türkiye'de Kültürü Yapılan Sinarit Balıklarında (Dentex dentex) İlk Vibrio harveyi Enfeksiyonu Bildirimi
Bu çalışmada, Türkiye'de yetiştiriciliği yapılan hasta sinarit balıklarından (Dentex dentex) Vibrio harveyi izole edilmiştir. Hastalık Temmuz ayında (2013), juvenil sinarit balıkları kuluçkahaneden yüzer kafes sistemlerine transfer edildikten sonra ortaya çıkmıştır. Hasta balıklardan 20-25 g ağırlığındaki 5 birey yüzer kafeslerden seçilmiş ve karaciğer, dalak, böbrek ve göz dokusundan örnekler alınmıştır. Bakteriyolojik kültür sonucunda 8 adet izolat saf olarak elde edilerek biyokimyasal karakterizasyonun yanı sıra 16S rRNA geni dizi analizi ile tanımlanmıştır. Bu çalışma, Türkiye'de kültürü yapılan sinarit balıklarında Vibrio harveyi enfeksiyonunun rapor edildiği ilk çalışmadır.
First Report of Vibrio harveyi Infection in Diseased Common Dentex (Dentex dentex) Cultured in Turkey
In the present work, Vibrio harveyi was consistently isolated from diseased common dentex (Dentex dentex) cultured in Turkey. The outbreak occurred in July (2013), following the transportation of common dentex juveniles from hatchery to offshore floating cages. Five moribund cultured common dentex individuals, about 20-25 g in weight, were selected from the said floating cages and samples were taken from internal organs including liver, spleen, kidney and eye tissue. A total of eight pure cultures were obtained and identified through basic biochemical characterisation as well as 16S rRNA gene sequencing. To our knowledge, this is the first report of Vibrio harveyi infection in cultured common dentex in Turkey.
___
- Abellán, E. 2000. Culture of Common Dentex (Dentex dentex L.): Present Knowledge, Problems and Perspectives. Cah Options Mediterr 47, 157-168.
- Austin, B., Zhang, X.H. 2006. Vibrio harveyi: A Significant Pathogen of Marine Vertebrates and Invertebrates. Lett Appl Microbiol, 43, 119-124.
- Balebona, M.C., Zorrilla, I., Moriñigo, M.A., Borrego, J.J. 1998. Survey of Bacterial Pathologies Affecting Farmed Gilt-Head Sea Bream (Sparus aurata L.) in Southwestern Spain from 1990 to 1996. Aquaculture, 166, 19-35.
- Clarridge, J.E. 2004. Impact of 16S rRNA Gene Sequence Analysis for Identification of Bacteria on Clinical Microbiology and Infectious Diseases. Clin Microbiol Rev., 17, 840-862.
- Company, R., Sitj, A., Pujalte, M., Garay, E., Alvarez-Pellitero, P. 1999. Bacterial and Parasitic Pathogens in Cultured Common Dentex, Dentex dentex L. Journal of Fish Diseases 22, 299- 309.
- Haldar, S., Maharajan, A., Chatterjee, S., Hunter, S., Chowdhury, N., Hinenoya, A., Asakura, M., Yamasaki, S. 2010. Identification of Vibrio harveyi as a Causative Bacterium for a Tail Rot Disease of Sea Bream Sparus aurata from Research Hatchery in Malta. Microbiological Research 165, 639-648.
- Hall, T.A. 1999. BioEdit: A User-Friendly Biological Sequence Alignment Editor And Analysis Program for Windows 95/98/NT. Proc. Nucleic Acids Symposium Series, 95-98.
- Hispano, C., Nebra, Y., Blanch, A.R. 1997. Isolation of Vibrio harveyi from an Ocular Lesion in the Short Sunfish (Mola mola). B Eur Assoc Fish Pat.
- Ishimaru, K., Muroga, K. 1997. Taxonomical Re-Examination of Two Pathogenic Vibrio Species Isolated from Milkfish and Swimming Crab. Fish Pathol, 32, 59-64.
- Kraxberger-Beatty, T., McGarey, D., Grier, H., Lim, D. 1990. Vibrio harveyi, an Opportunistic Pathogen of Common Snook, Centropomus undecimalis (Bloch), Held in Captivity. J Fish Dis., 13, 557-560.
- Larkin, M.A., Blackshields, G., Brown, N., Chenna, R., McGettigan, P.A., McWilliam, H., Valentin, F., Wallace, I.M., Wilm, A., Lopez, R. 2007. Clustal W and Clustal X Version 2.0. Bioinformatics 23, 2947-2948.
- López, J.R., De la Roca, E., Nşñez, S., De la Herran, R., Navas, J.I., Manchado, M., Herrera, M., Toranzo, A.E. 2009. Identification of Vibrio harveyi Isolated from Diseased Cultured Wedge Sole Dicologoglossa cuneata. Dis Aquat Organ, 84, 209-217.
- Pujalte, M., Sitja-Bobadilla, A., Macián, M., Belloch, C., Alvarez-Pellitero, P., Perez-Sanchez, J., Uruburu, F., Garay, E. 2003. Virulence and Molecular Typing of Vibrio harveyi Strains Isolated from Cultured Dentex, Gilthead Sea Bream and European Sea Bass. Syst Appl Microbiol., 26, 284-292.
- Qin, Y., Wang, J., Su, Y., Wang, D., Chen, X. 2006. Studies on the Pathogenic Bacterium of Ulcer Disease in Epinephelus awoara. Acta Oceanol. Sin., 25.
- Rueda, F., Martínez, F. 2001. A Review on the Biology and Potential Aquaculture of Dentex dentex. Reviews in Fish Biology and Fisheries, 11, 57-70.
- Saeed, M. 1995. Association of Vibrio harveyi with Mortalities in Cultured Marine Fish in Kuwait. Aquaculture, 136, 21-29.
- Sodimou, A.A., Alexis, M. 1998. Stress-Related Pathology Seems a Significant Obstacle for the Intensive Farming of Common Dentex, Dentex dentex (Linnaeus 1758). B. Eur. Assoc. Fish Pat., 18, 15.
- Suau, A., Bonnet, R., Sutren, M., Godon, J.-J., Gibson, G.R., Collins, M.D., Doré, J. 1999. Direct Analysis of Genes Encoding 16S rRNA from Complex Communities Reveals Many Novel Molecular Species Within the Human Gut. Applied and Environmental Microbiology, 65, 4799-4807.
- Tibaldi, E., Beraldo, P., Volpelli, L., Pinosa, M. 1996. Growth Response of Juvenile Dentex (Dentex dentex L.) to Varying Protein Level and Protein to Lipid Ratio in Practical Diets. Aquaculture, 139, 91-99.
- Whitman, K.A. 2004. Finfish and Shellfish Bacteriology Manual: Techniques and Procedures. Ames, USA: Iowa State Press.
- Zhang, Z., Schwartz, S., Wagner, L., Miller, W. 2000. A Greedy Algorithm for Aligning DNA Sequences. Journal of Computational Biology, 7, 203-214.
- Zorrilla, I., Arijo, S., Chabrillon, M., Diaz, P., Martinez-Manzanares, E., Balebona, M., Morinigo, M. 2003. Vibrio Species Isolated from Diseased Farmed Sole, Solea senegalensis (Kaup), and Evaluation of the Potential Virulence Role of Their Extracellular Products. J. Fish Dis. 26, 103-108.
- ISSN: 1300-4891
- Yayın Aralığı: Yılda 4 Sayı
- Başlangıç: 1988
- Yayıncı: SDÜ Eğirdir Su Ürünleri Fakültesi
Sayıdaki Diğer Makaleler
Thiacloprid ve D-Tubokurarin'in Kurbağa İskelet Kası Üzerine Ultrastrüktürel Etkileri
YUSUF ÇAMLICA, Esra PEKOĞLU, ŞAKİR NECAT YILMAZ
YUSUF ÇAMLICA, Esra PEKOĞLU, Serap YALIN
EMRE TURGAY, SÜHEYLA KARATAŞ STEINUM
Yunus Ömer BOYACI, Furkan DURUCAN
Akdeniz Bölgesi Sahil Şeridi Deniz Balıkçılığının Sosyoekonomik Yapısı