TARAKÇI Berrin GENÇER, KÖPRÜCÜ Sibel ŞİMŞEK

Regulatory peptides in gastroenteropancreatic endocrine cells of the rainbow trout (Oncorhynchus mykiss Walbaum,1792)

Gökkuşağı alabalığının gastroenteropankreatik endokrin hücreleri, parafın bloklarda immunohistokimyasal tekniklerin peroksidaz-anti-peroksidaz ve avidin-biotin-peroksidaz kompleks yöntemleri kullanılarak araştırıldı. Gastrin- ve substance P-immunoreaktif hücrelere pylorik mide bölümünde rastlanıldı. Substance P-immunoreaktif hücreleri ayrıca pylorik seka ve intestinal mukozada tesbit edildi. Glukagon içeren hücreler sadece bağırsakların başlangıç kısımlarında ve seyrek olarak da pylorik seka da gözlendi. Gastroenteropankreatik endokrin hücrelerinde calbindin immunoreaktivitesi bulunamadı. Pankreasda ise glukagon-ve insülin-immunoreaktivitesi tesbit edildi

Anahtar Kelimeler:

pankreas, peptit, bağırsaklar, balık, immünositokimya, endokrin sistem, mide

Gökkuşağı alabalığı (Oncorhynchus mykiss Walbaum,1792)'nın gastroenteropankreatik endokrin hücrelerindeki düzenleyici peptidler

Gastroenteropancreatic endocrine cells of rainbow trout have been investigated using the immunohistochemical techniques of peroxidase-anti-peroxidase and avidin-biotin-peroxidase complexes on parafin section. Gastrin- and substance P-immunoreactive cells were identified in the pyloric stomach region. Substance P-immunoreactive cells were also observed in intestinal mucosa and pyloric seca. Glucagon-like containing cells were restricted to the anterior intestine and rarely seen in pyloric seca. Calbindin-immunoreactivity was not find in gastroenteropancreatic endocrine cells. In pancreas, glucagon- and insulin-immunoreactivity were demonstrated.

Keywords:

pancreas, peptides, intestines, fishes, immunocytochemistry, endocrine system, stomach,

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