Bahaa ABDELLA, Eman EL-WAZZAN, Nermeen A. EL-SERSY, Soraya A. SABRY, Ehab R. EL-HELOW

Pathogenicity and antibiotic susceptibility of two bacterial pathogens associated with the clam Tapes decussatus in some Egyptian fisheries

Bivalve aquaculture is generally influenced by bacterial pathogens that cause high mortality-related losses in hatcheries. Six bacterial phenotypes had been previously isolated as the most predominant microorganisms in carpet shell clam (Tapes decussates) samples collected from natural beds in representative Egyptian clam fisheries. The main aims of this study were to investigate the nature of the pathogenic strains that significantly affect clam survival and test their susceptibility to commercially available antibiotics. Based on their 16S rRNA sequences and some biochemical features, two potent clam pathogens were emerged; one of which is gram-positive and the other is gram-negative. The former isolate was identified as Micrococcus luteus and the latter as Vibrio alginolyticus. Experimental challenges with the two bacterial pathogens introduced at different initial cell concentrations (2.5 X 104 - 7.5 X 104 cfu ml-1) showed markedly diverse clam mortality results. However, the pathogenic interaction of M. luteus with clam survival was generally higher than that of V. alginolyticus. When introduced at a relatively low initial cell density, the infectious records of V. alginolyticus were significantly increased suggesting that the expression of its key virulence factors is mainly triggered as a response to host contact. Antibiotic susceptibility tests suggested chloramphenicol and tetracycline as markedly effective agents that can be used to control the spread of these two bacterial pathogens in aquaculture applications

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