Oreochromis niloticus'un (Nil Tilapyası) solungaç ve kas dokularında civanın bazı enzimlere etkisi

Akuatik ekosistemler üzerine metallerin toksik etkileri balıklarda enzim aktivitelerinin ölçülmesiyle değerlendirilebilmektedir. Bu çalışmada civanın (Hg) Oreochromis niloticus (Linnaeus, 1758)'un solungaç ve kas dokularında asetilkolinestraz (AChE), glutatyon peroksidaz (GPx) ve glutamik piruvat transaminaz (GPT) aktivitelerine etkisi incelenmiştir. Balıklar 7 ve 21 günlük sürelerle 0,1 ve 0,01 ppm Hg konsantrasyonlarının etkisine maruz bırakılmıştır. Dokuların enzim aktiviteleri ultraviyole spektrofotometrik yöntem ile belirlenmiştir. Civa etkisinde AChE aktivitesi kontrol grubuna göre solungaç ve kas dokularında genel olarak azalırken GPx aktivitesi artmıştır. Kas GPT aktivitesi her iki sürede de kontrol grubuna göre artmıştır. Solungaçta GPT aktivitesi ise ilk 7 günlük sürede artarken 21 günlük sürede azalmıştır. Araştırma sonuçları düşük ve yüksek civa konsantrayonlarının Oreochromis niloticus'un solungaç ve kas dokularında AChE, GPx ve GPT aktivitelerinin değişmesine neden olduğunu göstermiştir.

Effects of mercury on some enzymes in the gill and muscle tissues ofOreochromis niloticus (Nile tilapia)

The toxic effects of the metals on the aquatic ecosystems can be found by measuring enzyme activities of fish tissues. This study aimed to analyze the results of mercury (Hg) exposure on the change of the asetylcolinestrase (AChE), glutation peroxidase (GPx) and glutamic piruvate transaminase (GPT) activities in gills and muscles of Oreochromis niloticus (Linnaeus, 1758). The fish were exposed to 0.1 and 0.01 ppm Hg for periods of 7 and 21 days. The enzyme activities in the tissues were determined by the use of ultraviolet spectroscopy. The AChE activity decreased while the GPx activity increased in both muscle and gills as a result to exposure to Hg in experimental group when compared to the control group. The GPT activity in gills were found to increase in the first 7 days of exposure and decreased after the exposure of 21 days. The data obtained revealed that AChE, GPx and GPT activities in the gill and muscle tissues of Oreochromis niloticus were observed to change as a result of the exposures of both low and high mercury concentrations.

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Alves, L.M., Lemos, M.F.L., Correiaa, J.P.S., Costaa, N.A.R. & Novais S.C. (2015). The potential of cholinesterases as tools for biomonitoring studies with sharks: Biochemical characterization in brain and muscle tissues of Prionace glauca.Journal of Experimental Marine Biology and Ecology, 465: 49-55. doi: 10.1016/j.jembe.2015.01.006
Badiou, A. & Belzunces, L.P. (2008). Is acetylcholinesterase a pertinent biomarker to detect exposure of pyrethroids? A study case with deltamethrin. Chemico-Biological Interactions, 25: 175(1-3):406-409. doi: 10.1016/j.cbi.2008.05.040
Berntssen, M.H.G, Aatland, A. & Handy, R.D. (2003). Chronic dietary mercury exposure causes oxidative stress, brain lesions, and altered behaviour in Atlantic salmon (Salmo salar). Aquatic Toxicology, 65: 55-72. doi: /10.1016/S0166-445X(03)00104-8
Beutler, E. (1984). Red cell metabolism: A manual of biochemical methods, 2nd ed., New York, Grune and Stratton.
Branco, V., Canario, J., Lu, J., Holmgren, A. & Carvalho, C. (2012). Mercury and selenium interaction in vivo: Effects on thioredoxin reductase and glutathione peroxidase. Free Radical Biology and Medicine, 52: 781-793. doi:10.1016/j.freeradbiomed.2011.12.002
Chen, C.Y., Wooster, G.S., Getchell, R.G., Bowser, P.R. & Timmons, M.B. (2003). Blood chemistry of healthy, nephrocalcinosis-affected and ozonetreated tilapia in a recirculation system, with application of discriminant analysis. Aquaculture, 218: 89-102. doi:10.1016/S0044-8486(02)00499-4
Costa, J.R.M., Mela, M., Assis, H.C.S., Pelletier, E., Randi, M.A.F. & OliveiraRibeiro, C.A. (2007). Enzymatic inhibition and morphological changes in Hoplias malabaricusfrom dietary exposure to lead (II) or methylmercury. Ecotoxicology and Environmental Safety, 67: 82-88.
De La Torre, F., Salibian, A. & Ferrari, L. (1999). Enzyme Activities as Biomarkers of Freshwater Pollution: Responses of Fish Branchial (Na +K) ATPase and Liver Transaminases. Environmental Toxicology, 14: 313- 319.
De La Torre, F., Salibian, A. & Ferrari, L. (2000). Biomarkers assessment in juvenile Cyprinus carpio exposed to waterborne cadmium. Environmental Pollution, 109: 277-282.
Ellman, G.L., Courtney, K.D., Andres, V. & Featherstone, R.M. (1961). A new and rapid colorimetric determination of acetycholinesterase activity. Biochemical Pharmacology, 7: 88-95.
Fırat, Ö. & Şahin İnandı, A. (2016). Investigation of combined effect of zeolite and mercury toxicity on some serum biochemical parameters of Oreochromis niloticus. Ege Journal of Fisheries and Aquatic Sciences, 33(3): 251-257. doi:10.12714/egejfas.2016.33.3.09
Harayashiki, C.A., Reichelt-Brushett, A.J., Liu, L. & Butcher, P. (2016). Behavioural and biochemical alterations in Penaeus monodon post-larvae diet-exposed to inorganic mercury. Chemosphere, 164: 241-247. doi: 10.1016/j.Chemosphere.2016.08.085
Hasspieler, B.M., Behar, I.V. & DiGiulio, R.T. (1994). Glutathionedependent defense in channel catfish (Ictalurus punctatus) and brown bullhead (Ameriurus nebulosus). Ecotoxicology and Environmental Safety, 28: 82- 90.
Hussain, S., Atkinson, A., Thompson, S.J. & Khan, A.T. (1999). Accumulation of mercury and it is effect on antioxidant enzymes in brain, liver and kidneys of mice. Journal of Environmental Science and Health B, 34: 645- 660. doi:10.1080/03601239909373219
Karthikeyan, S., Palaniappan, P.L.R.M. & Sabhanayakan, S. (2007). Influence of pH and water hardness upon nickel accumulation in edible fish Cirrhinus mrigala. Journal of Environmental Biology, 28:484-492.
Kumaresan, T. & Karuppasamy, R. (2011). Impact of industrial effluents on some biomarker enzymes in selected tissues of Arius maculates from Uppanar Estuary, Cuddalore District, Tamilnadu. Asian Journal of Science and Technology, 1(11): 70-75.Liao, C.Y., Fu, J.J., Shi, J.B., Zhou, Q.F., Yuan, C.G. & Jiang G.B. (2006).
Methylmercury accumulation, histopathology effects, and cholinesterase activity alterations in medaka (Oryzias latipes) following sublethal exposure to methylmercury chloride. Environmental Toxicology and Pharmacology, 22(2): 225-233. doi:10.1016/j.etap.2006.03.009
Lowry, O.H., Rosenbrough, N.J., Farr, A.L. & Randall, R.J. (1951). Protein measurement with the folin phenol reagent. Journal of Biological Chemistry, 193: 266-275.
Mazrouh, M.M. (2016). Effects of some heavy metals in different organs and some hepatic enzymes for European eel (Anguilla anguilla) at Lake Edku. International Journal of Science and Research, 5(2): 1872-1876.
Mary, S.C.H., Silvan, S. & Elumalai, E.K. (2014). Toxicology study on lead nitrate induced fresh water fish Cirrhinus mrigala (Hamilton). European Journal of Academic Essays 1(7): 5-8.
Mary, S.C.H., Bhuvaneswari, D. & Anandan, R. (2015). Biochemical and histopathological studies on lead nitrate induced toxicity in fresh water fish grass carp (Ctenopharyngodon idella). European Journal of Experimental Biology, 5(11): 24-30.
Monteiro D.A, Rantin F.T. & Kalinin A.L. (2010). Inorganic mercury exposure: toxicological effects, oxidative stress biomarkers and bioaccumulation in the tropical freshwater fish matrinxa, Brycon amazonicus (Spix and Agassiz, 1829). Ecotoxicology, 19:105-123. doi: 10.1007/s10695-010-9418-3
Reitman, S. & Frankel, S. (1957). A colorimetrik method for the determination of serum glutamic oxalacetic and glutamic pyruvik transaminases. American Journal of Clinical Pathology, 28: 56-63.
Saravanan, M., Kumar, K.P. & Ramesh, M. (2011). Haematological and biochemical responses of freshwater teleost fish Cyprinus carpio (Actinopterygii: Cypriniformes) during acute and chronic sublethal exposure to lindane. Pesticide Biochemistry and Physiology, 100(3): 206- 211. doi: 10.1016/j.pestbp.2011.04.002
Sevcikova, M., Modra, H., Slaninova, A. & Svobodova, Z. (2011). Metals as a cause of oxidative stress in fish: a review. Veterinary Medicine, 56(11): 537-546.
Sokal, R.R., & Rohlf, J.F. (1969). Biometry. Freeman and Company, San Fracisco, 776 pp.
Souid, G., Souayed N, Yaktiti, F. & Maaroufi, K. (2013). Effect of acute cadmium exposure on metal accumulation and oxidative stress biomarkers of Sparus aurata. Ecotoxicology and Environmental Safety, 89: 1-7. doi: 10.1016/j.ecoenv.2012.12.015
Tiwari, R., Wast, N., Siddiqui, A., Gaherwal S. & Prakash, M.M. (2014). Mercury and selenium induced changes in kidney biochemistry of Clarias batrachus. European Journal of Applied Sciences, 6 (4): 64-71. doi: 10.5829/idosi.ejas.2014.6.4.9160
Vaglio, A. & Landriscina, C. (1999). Changes in liver enzyme activity in the teleost Sparus aurata in response to cadmium intoxication. Ecotoxicology and Environmental Safety, 43: 111-116. doi: 10.1006/eesa.1999.1778
Vieira, L.R., Gravato, C., Soares, A.M.V.M, Morgado, F. & Guilhermino L. (2009). Acute effects of copper and mercury on the estuarine fish Pomatoschistus microps: linking biomarkers to behavior. Chemosphere, doi: 76: 1416-1427. 10.1016/j.chemosphere.2009.06.005
Zheng, J.L., Zeng, L., Xu, M.Y., Shen, B. & Wu, C.W. (2016). Different effects of low- and high-dose waterborne zinc on Zn accumulation, ROS levels, oxidative damage and antioxidant responses in the liver of large yellow croaker Pseudosciaena crocea. Journal of Physiology and Biochemistry, doi:10.1007/s10695-016-0275-6