FMC ve malaşit yeşili sağaltım dozlarının Oreochromis niloticus (L., 1758)' un bazı kan parametrelerinde meydana getirdiği değişimler
Bu araştırma, Nil Tilapyası {Oreochromis niloticus, L, 1758)'nda FMC (formalin, malaşit yeşili ve metilen mavisi karışımı) ve malaşit yeşili'nin oluşturduğu sekonder stres gelişimine ilişkin bazı kan parametrelerinde oluşan değişimler gözlemek amacıyla yapılmıştır. Bu kimyasal maddeler, özellikle tatlısu balıkları yetiştiriciliğinde, sıklıkla profilaktik ve dezenfektan olarak yaygın bir şekilde kullanılmaktadırlar. FMC ve malaşit yeşili, 1 mg/lt konsantrasyonunda hazırlanmış ve balıklar bu konsantrasyonlara 10 ve 60'ar dakikalık sürelerle daldırılmışlardır. Uygulama sürelerini takiben serumda glikoz, kortizol, laktat, kalsiyum, fosfor, magnezyum, sodyum, potasyum, klor ve hematokrit değerleri değerlendirilmiştir. Nil tilapya'sında FMC etkisinde serum fosfor, magnezyum, potasyum iyonları ile glikoz, kortizol, laktat düzeyleri, malaşit yeşili etkisinde ise serum glikoz ve kortizol düzeyleri kontrol grubuna göre artan süreye bağlı olarak yükselmiştir.
The variations in the some blood parameters caused by therapeutic concentration of FMC malachite green in Oreochromis niloticus (L., 1758)
This study was investigated to the variations in some blood parameters in relation to the seconder stres caused by FMC (the mixture of formalin, malachite green and methylene blue) and malachite green in Nile tilapia (Oreochromis niloticus, L, 1758). These chemicals are widely used especially in fresh water fish culture for prophylactic and disinfection purposes. FMC and malachite green were prepared as 1 mg/lt concentration and fish were immersed into the solutions for 10 and 60 minute durations. Following the immersion times, serum glucose, cortisol, lactate, calcium, phosphorus, magnesium, sodium, clore, potassium and hematocrite levels are measured. The serum phosphorus, magnesium, possium ions, glucose, cortisol and lactate levels were increased with FMC by time whereas only the serum glucose and cortisol levels were increased with malachite green by increasing immersion times in the Nile tilapia.
___
- Acerete, L, J.C. Balasch, E. Espinosa,, A. Josa,, L. Tort. 2004. Physiological responses in Eurasian perch (Perca fluviatialis, L.) subjected to stres by transport and handling. Aquaculture, 237:167-178.
- Atamanalp, M., A. Bayır, A.N. Sirkecioğlu, M. Cengiz. 2003. The Effects of Sublethal Doses a Disinfectant (Malachite Green) on Blood Parameters of Rainbow Trout {Oncorhynchus mykiss), (in Turkish). G.Ü. Gazi Eğitim Fakültesi Dergisi, 23 (3): 177-187.
- Bergwerff, AA, P. Scherpenisse. 2003. Determination of residues of malachite green in aquatic animals. Journal of Chromatography B, 788: 351-359.
- Biswas, A.K., M. Seoka, K. Takii, M. Maita, H. Kumai. 2005. Stress response i of red sea bream Pagrus major to acute handling and chronic photoperiod manipulation. Aquaculture, (Baskıda).
- Buhan, E., N. Akalın, H.Yılmaz, 1996. Fish health environmental relation and stress mechanism. Vet.Kontr. ve Araşt. Enst. Md. Derg. 20 (34): 173- 188.
- Kajimura, S., T. Hirano, S. Moriyama, O. Vakkuri, J. Leppaluoto, E.G. Grau. 2004. Changes in plasma concentrations of immunoreactive ouabain in the tilapia in response to changing salinity: is oubaine a hormone in fish. General and Comparative Endocrinology, 135:90-99.
- Larsson, A., C. Haux, Ml. Sjöbeck. 1985, Fish Phsiology and metal pollution: results and experiences from laboratory and field studies. Ecotoxicology and Environmental Safety, 9:250-281.
- Mathilakath, M.V., C. Pereira, E.G. Grau, G.K. Iwama. 1997. Metabolic responses associated with confinement stres in tilapia: the role of cortisol. Comp. Biochem. Physiol., 116 C, No 1:89-95.
- Meinelt, T., R. Playle, K. Schreckenbach, M. Pietrock. 2001. The toxicity of the antiparasitic mixture FMC is changed by humic substances and calcium. Aquaculture Research, 32:405-410.
- Quabius, E.S., P.H.M. Balm, S.E. Wendelaar Bonga. 1997. Interrenal stres responsiveness of tilapia {Oreochromis mossambicus) is impaired by dietary exposure to PCB 126. General and Comparative Endocrinology, 108:472-482.
- Schreck, C.B., W. Contreras-Sanchez, M.S. Fitzpatrick. 2001. Effects of stres on fish reproduction, gamete quality and progeny. Aquaculture, 197: 3- 24.
- Small, B.C. 2004. Effect of isoeugenol sedation on plasma cortisol, glucose and lactate dynamics in channel catfish Ictalurus punctatus exposed to three stressors. Aquaculture, 238:469-481.
- Srivastava, S., R. Sinha, D. Roy. 2004. Toxicolgical effects of malachite green. Aquatic Toxicology, 66:319-329.
- Yavuzcan Yıldız H. ve S. Pulatsii. 1999. Evaluation of the secondary stres response in healthy Nile tilapia (Oreochromis niloticus, L) after treatment with a mixture of formalin, malachite green and methylene blue. Aquaculture Research, 30:379-383.
- Yılmaz, B. 1984. Physiology (in Turkish), Hacettepe Taş Kitapçılık Ltd. Şti., Ankara.