Farklı besin ortamının ve farklı ışık yolu uzunluğunun Chlorella vulgaris kültüründe etkisi

Bu araştırmada Chlorella vulgaris kültürlerinde farklı ortamların ve farklı ışık yolu uzunluğuna sahip olan panel fotobiyoreaktörlerin etkileri incelenmiştir. Jaworski besin ortamında hızlı bir hücre artışı tespit edilmiştir (60,8 x 106 hücre mL-1 ). F/2 besin ortamında ise 11. günde hücre sayısı 1,37 x 106 hücre mL-1 olarak kayıt edilmiştir. Jaworski ve F/2 besin ortamlarında yetişen kültürlerin kuru ağırlık değeri 11. gün sonunda 0,46 gL-1 , 0,13 gL-1 olarak ölçülmüştür. Cam panel biyoreaktör denemelerinde en yüksek hücre sayısına 31. günde 1 cm ışık yolu uzunluğuna sahip biyoreaktörde ulaşılmıştır (230 x 106 hücre mL-1 ). Fakat 3 cm ve 5cm ışık yoluna sahip biyoreaktörlerde ise en yüksek hücre sayısı olduğu değer 90 x 106 hücre mL-1 olarak tespit edilmiştir. 1, 3 ve 5 cm ışık yolu uzunluğuna sahip biyoreaktörlerde maksimum kuru ağırlık değerleri ise sırasıyla 3,6 gL-1 , 2,8 gL-1 ve 2,4 gL-1 olarak tespit edilmiştir. Panellerde yapılan Chlorella vulgaris kültürlerin klorofil a değerleri zamanla düştüğü tespit edilmiştir. Aynı zamanda yapılan denemede büyüme oranları ile klorofil a değerlerinin ters orantılı olduğu gözlenmiştir.

The effect of different growth medium and different light path length on the culture of Chlorella vulgaris

In this study, the effects of panel photobioreactors with different nutrient mediums and different light path lengths on Chlorella vulgaris cultures were investigated. Rapid cell growth was detected in the Jaworski nutrient medium (60.8 x 106 cells mL-1 ). In the F / 2 nutrient medium, the cell number was recorded as 1.37 x 106 cells mL-1 on the 11th day. Dry weight of cultures grown in Jaworski and F / 2 nutrient media were measured as 0.46 gL-1 and 0.13 gL-1 at the end of 11 days, respectively. At the experiments in glass panel bioreactor, the highest cell number was found on the 31st day (230 x 106 cells mL-1 ) in a bioreactor with 1 cm light path length. Whereas, the highest cell numbers was found as 90 x 106 cells mL-1 in glass panel bioreactors with 3 cm and 5 cm light path length. The maximum dry weights for bioreactors with 1, 3 and 5 cm light path lengths were determined as 3.6 gL-1 , 2.8 gL-1 and 2.4 gL-1 , respectively. It was also determined that chlorophyll a values of Chlorella vulgaris on the panels bioreactors decreased over time. Moreover, the inversely proportion between growth rates and the chlorophyll-a values was observed.

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