Farklı Artemia spp. yoğunluklarında ultraviyole ışınları kullanımının bakteri yükü üzerine etkisi

Bu çalışmada, ticari balık üretiminde kullanılan farklı Artemia (Artemia spp.) yoğunluklarında (yaklaşık 260 - 4500 adet.ml-1) ve farklı su akış debilerinde (0.63- 1.01 – 1.55 – 1.98 lt.dak-1), U.V. ışımasının ölüm oranlarına ve bakteri yükündeki azalma oranlarına etkileri tespit edilmiştir. Mikrobiyolojik inceleme, toplam bakteri TSA agar, Vibrio spp. TCBS agar besiyerlerine yapılan ekimlerle değerlendirilmiştir. Artemia’ların yaşama yüzdeleri, U.V. dezenfeksiyonu öncesi ve sonrasında ışık mikroskobu altında hesaplanmıştır. Araştırma süresince Artemia yoğunlukları 260±26 adet/ml ile 4500±412 adet/ml arasında tespit edilmiştir. Ortalama total bakteri yükü, TCBS agarda %56.6±5.4 ile %84.3±3.5 oranında ve TSA agarda %66.3±9.8 ile %77.9±3.8 arasında elimine edilmiştir. Ortalama Artemia ölüm oranları %4.4±1.8 ile %9.97±6.61 arasında tespit edilmiştir.

The effects of ultraviolet radiation on bacterial ioad of different density of Artemia spp

The effects of ultraviolet radiation on bacterial load of the different density of Artemia spp. In this study, the effects of ultraviolet radiation on bacterial load and survival rates of Artemia at different densities (250- 4500 ind.ml-1) used in commercially marine fish production, and at flow rates (0.63- 1.01 - 1.55 - 1.98 lt.min-1) was determined. TSA agar and TCBS agar were used to calculate for total bacteria and Vibrio spp. bacteria, respectively. The mortality of the Artemia was calculated by light microscope. The Artemia density was estimated as 260±26 and 4500±412 ind.ml-1 during the experiments. The average load of the total bacteria was eliminated between 56.6±5.4% and 84.3±3.5% in TCBS agar and 66.3±9.8% and 77.9±3.8% in TSA agar. The average mortality of Artemia was obtained between 4.4±1.8 % and 9.97±6.61 %.

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Austin, B., D. A. Allen, 1982. The microbiology of laboratory hatched brine shrimp Artemia. Aquaculture, 26, 369–383.

Bachere, E. 2003. Anti-infectious immune effectors in marine invertebrates: potential tools for disease control in larviculture. Aquaculture, 227, 427– 438.

Başaran, F., Ö. Ilgaz, B. Tülek, and G. Muhtaroğlu, 2006. The effects of using ultraviolet radiation on bacterial load of the different density of rotifer (Brachionus plicatilis). (in Turkish) E.U. Journal of Fisheries and Aquatic Sciences, 23 (1-2), 55-59.

Bridges, B. A. 1976. Survival of bakteri following exposure to ultraviolet and ionizing radiations, p. 183-208. In T. R. Gray and J. R. Postgate [eds.], The survival of vegetative microbes. Cambridge Univ. Cambridge.

Brown, C., D. J. Russo, 1979. Ultraviolet light disinfection of shellfish hatchery seawater. Aquaculture, 176, 17-23

Burrows, R. E., B. D. Combs, 1968. Controlled environments for salmon propagation. The Progressive Fish. Culturist, 30, 123-136.

Defoirdt, T., P. Bossier, P. Sorgeloos and W. Verstraete, 2005. The impact of mutations in the quorum sensing systems of Aeromonas hydrophila, Vibrio anguillarum and Vibrio harveyi on their virulence towards gnotobiotically cultured Artemia franciscana. Environ. Microbiol. 7 (8) 1239–1247.

Dehasque, M., L. Verdonck, P. Sorgeloos, J. Swings, P. Leger and K. Kristers, 1991. Determination of the bacterial contamination in live food production system and in marine fish hatcheries in Souther Europe. In: Lavens, P., Sorgeloos, P., Jaspers, E., Oliver F. Eds. , Larvi-91. Fish and Crustacean Larviculture Symposium, European Aquaculture Society Special Publication No. 15, Ghent, Belgium, 399–401.

Frappaolo, P.J., G. B. Guest, 1986. Regulatory status of tetracyclines, penicillin and other antimicrobial drugs in animal feeds. J. Anim. Sci., 62, 86–92.

Gatesoupe, F. J. 1990. The contionus feeding and turbot larvae Scophthalmus maximus and control of thebacterial environment of rotifers. Aquaculture, 89, 139-148.

Gomezgil, B. R. S., F. A. A. Grobois, J. R. Jarero and M. D. H. Vega, 1994. Chemical disinfection of Artemia nauplii. J. World Aquacult. Soc., 25 (4) 574–583.

Hatai, K., S. Yasumoto, S. Ogawa, and N. Yasunaga, 1981. Elimination of bacteria associated with baking yeast-fed rotifers by some antibacterial agents. Rep. Nagasaki Prefect. Fish. Stn., 218–222.

Munro, P. D., A. Barbour and T. H. Birkbeck, 1994. Comparison of the gut bacterial flora of start-feeding larval turbot reared under different conditions. J. Appl. Bacteriol., 7, 560-566.

Munro, P. D., R. J. Handerson, A. Barbour and T. H. Birkbeck, 1999. Partial decontamination of rotifers with ultraviolet radiation: the effect of changes in the bacterial load and flora of rotifers on mortalities in start-feeding larval turbot. Aquaculture, 170, 229-244.

Marques, A., Thanh, T. H., Verstraete, W., Dhont, J., Sorgeloos, P., Bossier, P., 2006. Use of selected bacteria and yeast to protect gnotobiotic Artemia against different pathogens. Journal of Experimental Marine Biology and Ecology. 334(1) 20-30.

Muroga, K., M. Higashi and H. Keitiku, 1987. The isolation of intestinal microflora of farmed red seabream. Pagrus major and black seabream Acanthopagrus schlegeli of larval and juvenile stages. Aquaculture, 65, 79–88.

Nicolas, J. L., E. Robic, and D. Ansquer, 1989. Bacterial flora associated with a trophic chain consisting of microalgae, rotifers and turbot larvae: influence of bacteria on larval survival. Aquaculture, 83, 237–248.

Rodriguez, J. L., M. Planas and J. J. Otero, 1991. Microflora and antibacterial treatments of rotifers and Artemia. In: Lavens, P., Sorgeloos, P., Jaspers, E., Olleveier, F. Eds. Larvi-91. Fish and Crustacean Larviculture Symposium. European Aquaculture Society, Special Publication No. 15, 403–405, Ghent, Belgium.

Rosenthal, H. 1993. The history of recycling technology: A lesson learned from past experience. In: Fish Farming Technology (ed. by H. Reinertsen, L.A. Dahle, L. Jurgensen), 341-349, Balkema, Rotterdam.

Sakai, M. 1999. Current research status of fish immunostimulants. Aquaculture, 172, 63–92.

Sahul Hamed, A. S., G. Balasubramanian, 2000. Antibiotic resistance in bacteria isolated from Artemia nauplii and efficacy of formaldehyde to control bacterial load. Aquaculture, 183, 195–205.

Savaş, S., and Ş. Gökpınar, 2002. The quantitative determination of aerobic bacterial flora in rotifer (Brachionus plicatilis) in large scale rotifer cultures. (in Turkish) E.U. Journal of Fisheries and Aquatic Sciences, 19(1-2) 97-103.

Skejermo, J., O. Vadstein. 1993. The effect of microalgae on skin and gut bacterial flora of halibut larvae. Fish Farming Technology Ed. By. Reinertsen H, Dahle, L.A., Jargensen L., and Tvinnereim, The Research Council of Norway, 61 –67.

Skejermo, J., I. Salvasen, G. Qie, Y. Olsen and O. Vadstein, 1997. Microbially matured water: a technique for selection of a non-opportunisticbacterial flora in water that may improve performance of marine larvae. Aquaculture International, 5,13-28.

Sorgeloos, P., P. Lavens, P. Legar, Tackaert, W., Versichele, D., 1986. Manual for the culture and use of brine shrimp Artemia in Aquaculture. Artemia, Reference Centre, State University of Ghent, Belgium.

Tatani, M., K. Muroga, T. Sugiyama, and Y. Hiramoto, 1985. Detection of Vibro anguillarum from reared fry and fingerlings of ayu. Suisan Zoshoku, 33, 59–66.

Tanasomwang, V., K. Muroga, 1989. Effects of sodium nifurstyrenate and tetracycline on the bacterial flora of rotifers Brachionus plicatilis . Fish Pathol., 24, 29–35.

Tanasomwang, V., K. Muroga, 1992. Effect of sodium nifurstyrenate on the reduction of bacterial contamination of rotifers Brachionus plicatilis . Aquaculture, 103, 221–228.

Tolomei, A., C. Burke, B. Crear, and J. Carson, 2004. Bacterial decontamination of on-grown Artemia. Aquaculture, 232,357-371.

Vadstein, O., G. Qui, Y. Olsen, I. Salvesen, J. Skejermo and G. Skjak-Braek, 1993. A strategy to obtain microbial control during larval development of marine fish. Fish Farming Technology, 69-75. Rotterdam.

Verdonck, L., M. Dehasque, J. Swings, P. Sorgeloos and P. Leger, 1991. The microbial environment of rotifer. Brachionus plicatilis and Artemia production systems. In: Lavens, P., Sorgeloos, P., Jaspers, E., Ollevier, F. Eds. , Larvi-91, Fish and Crustacean Larviculture Symposium. European Aquaculture Society, Special Publication No. 15, 398, Ghent, Belgium.

Verschuere, L., H. Heang, G. Criel, P. Sorgeloos and W. Verstraete, 2000. Selected bacterial strains protect Artemia spp. from pathogenic effects of Vibrio proteolyticus CW8T2. Appl. Environ. Microbiol. 66 (3) 1139–1146.

Yamanoi, H., T. Sugiyama, 1987. Effects of sodium nifurstyrenate bath and ultraviolet irradiation on the elimination of bacteria associated with rotifer. Suisan Zoshoku, 35, 190–195.
Su Ürünleri Dergisi-Cover
  • ISSN: 1300-1590
  • Yayın Aralığı: 4
  • Başlangıç: 1984
  • Yayıncı: Aynur Lök