LEYLA USLU, Oya IŞIK, Burcu AK

Düz panel reaktör sistemde Porphyridium purpureum’un dışarı kültürü ve polisakkarit eldesi

Çalışmada Porphyridium purpureum türü dışarı ortamda farklı ışık yolu uzunluğuna (3, 5, 7 cm) sahip düz panel fotobiyoreaktör sistemlerinde kültüre alınmıştır. Lipid, protein, biyomas ve polisakkarit miktarları belirlenmiştir. Porphyridium purpureum türü için en yüksek polisakkarit miktarı (0,48 gL-1 ), 1,316 gL-1 biyomas ve %29,7 protein içeriği ile 3 cm ışık yolu uzunluğuna sahip düz panel photobiyoreaktör sistemde belirlenmiştir. En düşük polisakkarit miktarı 0,31 gL-1 ve en yüksek protein %32,3 ile 7 cm ışık yoluna sahip photobiyoreaktör sistemde belirlenmiştir. Sonuç olarak, Porphyridium purpureum türünün Çukurova (Adana, Türkiye) koşullarında üretimi, panel sistemlerde başarılı bir şekilde tamamlanmıştır. En iyi büyüme 3 cm ışık yoluna sahip panel fotobiyoreaktörde elde edilmiştir.

Outdoor culture and polysaccharide extraction of Porphyridium purpureum in flat panel reactor system

In this study, Porphyridium purpureum was cultured in a flat panel photobioreactor systems, with different light path (3, 5 and 7 cm). Lipid, protein, biomass and polysaccharide rate were determined. The highest polysaccharide content (0.48 gL-1 ) for Porphyridium purpureum in the study was obtained from the culture with 1.316 gL-1 biomass rate, 29,7% protein it the panel PBR system with 3 cm light path. The lowest polysaccharide content and highest protein were found as 0.31 gL-1 , 32.3% respectively, at it the panel PBR system with 7 cm light path. Consequently, Porphyridium purpureum cultured successfully completed in flat panel photobioreactor system at Cukurova Conditions (Adana, Turkey). The best growth was obtained from 3 cm light path photobioreactor system.

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AOAC Method. (1995). Official methods of analysis, 16th edn. In: Cuniff P (ed) AOAC International, Arlington, VA, USA, pp:32–33
Becker, E.W. (1994). Microalgae: Biotechnology and Microbiology (Vol. 10). Cambridge University Press.
Bligh, E.G. & Dyer, W.J. (1959). A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and physiology, 37(8):911-917. DOI: 10.1139/y59-099
Brown, M.R., Jeffrey, S.W., Volkman, J.K. & Dunstan, G.A. (1997). Nutritional properties of microalgae for mariculture. Aquaculture, 151(1-4):315-331. DOI: 10.1016/S0044-8486(96)01501-3
Cirik, S. & Gökpınar, Ş. (1993). Plankton Bilgisi ve Kültürü. Ege Üniversitesi Su Ürünleri Fakültesi Yayınları, 47:131-133.
Cohen, E. & Arad, S.M. (1989). A closed system for outdoor cultivation of Porphyridium. Biomass, 18(1): 59-67. DOI: 10.1016/0144- 4565(89)90081-4
Cohen Z., Vonshak, A., Boussiba S. & Richmond, A. (1988). The effect of temperature and cell concentration on the fatty acid composition of outdoor cultures of Porphyridium cruentum. In: Stadler T, Mollion J, Verdus M-C, Karamanos Y, Morvan H, Christiaen D (eds.), Algal Biotechnology (pp 421–430). Elsevier Applied Science, London.
Dyerberg, J. (1986). Linolenate‐derived Polyunsaturated Fatty Acids and Prevention of Atherosclerosis. Nutrition Reviews, 44(4):125-134. DOI:10.1111/j.1753-4887.1986.tb07603.x
Gantt, E. (1981). Phycobilisomes. Annual Review of Plant Physiology, 32(1): 327-347.
Geresh, S. & Arad, S. (1991). The extracellular polysaccharides of the red microalgae: chemistry and rheology. Bioresource Technology, 38(2- 3):195-201. DOI: 10.1016/0960-8524(91)90154-C
Gill, I. & Valivety, R. (1997). Polyunsaturated fatty acids, part 1: occurrence, biological activities and applications. Trends in Biotechnology, 15(10):401-409. DOI: 10.1016/S0167-7799(97)01076-7
Goldman, J.C. (1979). Outdoor algal mass cultures—I. Applications. Water Research, 13(1):1- 19. DOI: 10.1016/0043-1354(79)90249-5
Golueke, C. G. & Oswald, W. J. (1962). The mass culture of Porphyridium cruentum. Applied Microbiology, 10(2):102-107.
Guillard, R.R.L. (1973). Division Rates. In: Stein, R.J. (Ed.) Handbook of Phycological Methods: Culture Methods and Growth Measurements. Cambridge University Press.
Gunstone, F.D. (1992). Gammar linolenic acid—occurrence and physical and chemical properties. Progress in Lipid Research, 31(2):145-161. DOI:10.1016/0163-7827(92)90007-6
Horrobin, D.F. (1992). Nutritional and medical importance of gamma-linolenic acid. Progress in Lipid Research, 31(2):163-194. DOI:10.1016/0163-7827(92)90008-7
Innis, S.M. (1991). Essential fatty acids in growth and development. Progress in Lipid Research, 30(1):39-103. DOI:10.1016/0163-7827(91)90006-Q
Jones, R.F., Speer, H.L. & Kury, W. (1963). Studies on the growth of the red alga Porphyridium cruentum. Physiologia Plantarum, 16(3):636-643. DOI: 10.1111/j.1399-3054.1963.tb08342.x
Kusmiyati, K. & Agustini, N.W.S. (2007). Antibacterial activity assay from Porphyridium cruentum microalgae. Biodiversitas Journal of Biological Diversity, 8(1):48-53.
Nettleton, J.A. (1993). Are n-3 fatty acids essential nutrients for fetal and infant development?. Journal of the American Dietetic Association, 93(1):58-64. DOI: 10.1016/0002-8223(93)92132-H
Parsons, T.R. (1963). Discussion of spectrophotometric determination of marine plant pigments, with revised equation for ascertaining chlorophylls and carotenoids. J. Mar. Res., 21:155-163.
Ramus, J. & Groves, S.T. (1972). Incorporation of sulfate into the capsular polysaccharide of the red alga Porphyridium. The Journal of Cell Biology, 54(2):399-407. DOI: 10.1083/jcb.54.2.399
Ramus, J., Kenney, B.E. & Shaughnessy, E.J. (1989). Drag reducing properties of microalgal exopolymers. Biotechnology and Bioengineering, 33(5):550-557. DOI: 10.1002/bit.260330506
Rebolloso Fuentes M.M., Garcia Sanchez, J.L., Fernandez, Sevilla J.M., Acien Fernandez, F.G., Sanchez Perez, J.A., Molina Grima, E. (1999). Outdoor continuous culture of Porphyridium cruentum in a tubular photobioreactor: quantitative analysis of the daily cyclic variation of culture parameters. Journal of Biotechnology, 70: 271–288.
Schlegel, H.G. (2007). Allgemeine mikrobiologie. Georg Thieme Verlag. Simopoulos, A.P. (1991). Omega-3 fatty acids in health and disease and in growth and development. The American Journal of Clinical Nutrition, 54(3):438-463. DOI: 10.1093/ajcn/54.3.438
Singh, S., Arad, S.M. & Richmond, A. (2000). Extracellular polysaccharide production in outdoor mass cultures of Porphyridium sp. in flat plate glass reactors. Journal of Applied Phycology, 12(3-5):269-275.
Singh, G. & Chandra, R. K. (1988). Biochemical and cellular effects of fish and fish oils. Progress in Food & Nutrition Science, 12(4): 371-419.
Sukenik, A. (1991). Ecophysiological considerations in the optimization of eicosapentaenoic acid production by Nannochloropsis sp.(Eustigmatophyceae). Bioresource Technology, 35(3): 263-269. DOI: 10.1016/0960-8524(91)90123-2.
Vonshak, A. (1988). Porphyridium. In: Borowitzka MA, Borowitzka LJ (eds) Micro-algal Biotechnology (pp 122–134). Cambridge University Press, Cambridge.
You, T. & Barnett, S.M., (2004). Effect of light quality on production of extracellular polysaccharides and growth rate of Porphyridium cruentum. Biochemical Engineering Journal. 19: 251-258.
Zarr, J.H. (1999). Biostatistical analysis. Prentic Hall. Upper Saddle River, New Jersey. Pg, 161-164.