Dişi Van balığı (Alburnus tarichi, Guldenstaedtii, 1814)’nda sitokrom P450 aromataz izoformlarının ekspresyonu

Bu çalışmanın amacı, Van Gölü’nden yakalanan dişi Alburnus tarichi’de P450 aromataz izoformları olan beyin kaynaklı Cyp19b ve ovaryum kaynaklı Cyp19a genlerini klonlanmak ve üreme bakımından ovaryum gelişimi engellenmiş (RA) ve ovaryum gelişimi normal olan (RN) balıklardan alınan hypothalamus ve ovaryum örneklerinde bu genlerin ekspresyon seviyelerini belirlemektir. Cyp19b gen ekspersyonu beyinde, Cyp19a gen ekspresyonu ise ovaryumda daha fazlaydı. Cyp19b mRNA seviyesi, RA balıkların ovaryum ve hipotalamusunda, RN balıkların ovaryum ve hipotalamusundan belirgin olarak düşüktü (P

Expression of cytochrome P450 aromatase isoforms in female Alburnus tarichi (Guldenstaedtii, 1814)

In this study, we aimed to clone brain-derived Cyp19b and ovary-derived Cyp19a the P450 aromatase gene isoforms and to indicate the expression levels of these genes in the hypothalamus and ovary tissues from reproductively arrested ovarian development (RA) and non-arrested ovarian development (RN) Alburnus tarichi from Lake Van, Turkey. The expression levels of Cyp19b and Cyp19a genes were predominant in the hypothalamus and ovary, respectively. The level of Cyp19b mRNA was significantly lower in the hypothalamus and ovary from RA fish than in the hypothalamus and ovary from RN fish (P

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