Azot kaynakları ve konsantrasyonlarının Nannochloropsis oculata (Droop, 1955) (Eustigmatophyceae)'nın büyüme ve pigment komposizyonuna etkisi

Azot kaynakları ve konsantrasyonlarının Nannochloropsis oculata türünün karotenoidler ve klorofil a üretimi üzerine etkileri incelendi. N. oculata, laboratuvarda 2 L’lik erlenlerde, F/2 besin ortamı ile zenginleştirilmiş steril deniz suyunda yığın kültürleri yapıldı (Tuzluluk % 2.5). Kültürler 18±1ºC sabit sıcaklıkta tutuldu. Azot kaynakları olarak sodyum nitrat, amonyum klorür ve üre olarak kullanıldı. Azot kaynakları besin ortamlarına 882 ve 441 μmol L-1 konsantrasyonlar kullanıldı. Denemeler sonunda β-Karoten, klorofil-a, klorofil-b, violaksantin, kantaksantin, astaksantin, vokeriyaksantin ve zeaksantin N.oculata’da ana pigmentler olarak TLC yöntemi ile saptandı. Toplam karotenoidler ve klorofil a düzeyleri bakımından en yüksek değerler 882 μmol L-1 NO3- -N ile hazırlanan besin ortamında ilk alınan örneklerde elde edildi (değerler sırasıyla, 4.17±0.37 mg g-1 dw ve 12.42±1.08 mg g-1 dw ). N. oculata türü için inorganik azot kaynaklarından NO3- -N ve NH4+-N ile büyüme ve biyokimyasal yapısı açısından benzer sonuçlar elde edildi. Fakat üre-N’nun bu tür için uygun kaynak olmadığı tespit edildi.

Effects of nitrogen sources and concentration on the growth and pigment composition of the Nannochloropsis oculata (Droop, 1955) (Eustigmatophyceae).

Effects of nitrogen sources and concentration on the growth and pigment composition of the Nannochloropsis oculata (Droop, 1955) (Eustigmatophyceae). Effects of nitrogen sources and concentration on the production of carotenoids and chlorophyll a of the Nannochloropsis oculata were examined. N. oculata were grown in laboratory, 2 L erlens as batch cultures in sterilized seawater enriched with F/2 medium (salinity 2.5 %). Cultures were kept at 18±1ºC under controlled temperature conditions. Sodium nitrate, ammonium chloride and urea were used as the nitrogen sources. It was arranged combinations of two different concentrations in the medium (882 and 441 μmol L-1). The main pigments of N. oculata were determined by TLC method as β-carotene, chlorophyll a, chlorophyll b, violaxanthin, canthaxanthin, astaxanthin and vaucheriaxanthin. Total carotenoids and chlorophyll a were highest at 882 μmol L-1 NO3--N in first harvest samples (4.17±0.37 mg g-1 dry weight (dw) and 12.42±1.08 mg g-1 dw, respectively). NO3- -N and NH4+-N as a inorganic nitrogen sources for N. oculata obtained same growth rate and biochemical compotion. But urea-N as a nitrogen source for this species determined to be unsuitable source.

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