Aquatik bir bitkinin (Riccia fluitans) toplam fenolik ve toplam flavonoid içeriklerinin ve antioksidan kapasitelerinin belirlenmesi

Genel adı crystalwort olan Riccia fluitans, akvaryum bitkileri arasında popüler olan Riccia kızılyaprak cinsinden suda yüzen bir bitkidir. Riccia fluitans sık sık su yüzeyinde ve altında kalın paspaslar oluşturarak ve havuzlarda yüzüyor olarak bulunabilir. Bu çalışmanın amacı, Riccia fluitans' ın farklı in vitro yöntemlerle antioksidan aktivitelerinin belirlenmesidir. Antioksidan ve antiradikal aktiviteler değerlendirilmesi için, ABTSo+ (2,2 '-azino - bis (3- etilenbenztiyazolin -6-sülfonik asit) radikali giderme aktivitesi, DPPHo (1,1- difenil-2- pikril-hidrazil) serbest radikal giderme aktivitesi, indirgeme kuvveti ve demir iyonları şelatlama aktiviteleri hesaplandı. ? -tokoferol referans antioksidan bileşik olarak kullanılmıştır. Buna ek olarak, etanol ekstrelerinde toplam fenolik ve toplam flavonoid bileşikler sırasıyla, gallik asit ve kuersetin eşdeğerleri olarak belirlenmiştir. Sonuç olarak, bu bitki doğal gıda antioksidan umut verici bir kaynağıdır

Determination of total phenolic and total flavonoid contents and antioxidant capacities of an aquatic plant (Riccia fluitans)

Riccia fluitans, whose common name is crystalwort, is an aquatic floating plant of the liverwort genus Riccia which is popular among aquarist plants. It can be found floating in ponds, and often forms thick mats on and under the water surface. The objective of this study made to determine the antioxidant activities of Riccia fluitans using different in vitro methods. For evaluation of antioxidant and antiradical activities, ABTS•+ (2,2´-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)) radical scavenging activity, DPPH• (1,1-diphenyl-2-picryl-hydrazyl) free radical scavenging activity, reducing power, and ferrous ions chelating activities were calculated. α-tocopherol were used as the reference antioxidant compound. In addition, the total phenolic and total flavonoid compounds in ethanol extracts were determined as gallic acid and quercetin equivalents, respectively. Consequently, this plant is a promising source of natural food antioxidants

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Ak, T., Gulcin, İ., 2008. Antioxidant and radical scavenging properties of curcumin. Chemico-Biological Interactions. 174: 27-37. doi: 10.1016/j.cbi.2008.05.003
Blois, M.S., 1958. Antioxidant determinations by the use of a stable free radical. Nature. 26: 1199-1200. doi:10.1038/1811199a0
Cecal, A., Popa, K., Caraus, I., Potoroaca, V., 2002. Bioaccumulation of 65Zn2+ ions by some hydrophytic plants. Isotopes in Environmental and Health Studies. 38: 33-37.
Chojnacka, K., 2007. Biosorption and bioaccumulation of microelements by Riccia Xuitans in single and multi-metal system. Bioresource Technology. 98: 2919-2925. doi: 10.1016/j.biortech.2006.10.001
Couladis, M., Tzakou, O., Verykokidou, E., Harvala, C., 2003. Screening of some Greek aromatic plants for antioxidant activity. Phytotherapy Research. 17: 194-195. doi: 10.1002/ptr.1261
Dinis, T.C.P., Madeira, V.M.C., Almeida, L.M., 1994. Action of phenolic derivates (acetoaminophen, salycilate, and 5-aminosalycilate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Archives of Biochemistry and Biophysics. 315: 161-169. doi: 10.1006/abbi.1994.1485
Gutteridge, J.M.C., 1994. Biological origin of free radicals and mechanisms of antioxidant protection. Chemico-Biological Interactions. 91: 133-140. doi:10.1016/0009-2797(94)90033-7
Gulcin, I., Elias, R., Gepdiremen, A., Boyer, L., 2006a. Antioxidant activity of lignans from fringe tree (Chionanthus virginicus L.). European Food Research and Technology. 223: 759-767. doi: 10.1007/s00217-006-0265-5
Gulcin, I., Kirecci, E., Akkemik, E., Topal, F., Hisar, O., 2010. Antioxidant, antibacterial, and anticandidal activities of an aquatic plant: duckweed (Lemna minor L. Lemnaceae). Turkish Journal of Biology. 34: 175-188.
Gulcin, I., Mshvildadze, V., Gepdiremen, A., Elias, R., 2006b. Screening of antioxidant and antiradical activity of monodesmosides and crude extract from Leontice smirnowii Tuber. Phytomedicine. 13: 343-51. doi:10.1016/j.phymed.2005.03.009
Gulcin, I., Sat, I.G., Beydemir, S., Kufrevioglu, I., 2004. Evaluation of the in vitro antioxidant properties of extracts of broccoli (Brassica oleracea L.). Italian Journal of Food Science. 16: 17-30.
Gulcin, I., 2006c. Antioxidant activity of caffeic acid (3,4- dihydroxycinnamic acid). Toxicology. 217: 213-220. doi: 10.1016/j.tox.2005.09.011 Gulcin, I., 2009. Antioxidant activity of L-Adrenaline: An activity structure insight. Chemico-Biological Interactions. 179: 71-80. doi: 10.1016/j.cbi.2008.09.023
Gulcin, I., 2006a. Comparison of in vitro antioxidant and antiradical activities of L-tyrosine and L-Dopa. Amino Acids. 32: 431-438. doi: 10.1007/s00726-006-0379-x.
Gulcin, I., 2005. The antioxidant and radical scavenging activities of black pepper (Piper nigrum) seeds. International Journal of Food Sciences and Nutrition. 56: 491-499. doi:10.1080/09637480500450248
Hsieh, R.J., Kinsella, J.E., 1989. Oxidation of polyunsaturated fatty acids: Mechanisms, products, and inhibition with emphasis on fish. Advances in Food and Nutrition Research. 33: 233-241. doi:10.1016/S1043-4526(08)60129-1
Katalinic, V., Milos, M., Kulisic, T., Jukic, M., 2006. Screening 70 medicinal plant extracts for antioxidant capacity and total phenols. Food Chemistry. 94: 550-557. doi: 10.1016/j.foodchem.2004.12.004 Koksal, E., Gulcin, İ., Ozturk, S.B., Bursal, E., 2008. On the in vitro antioxidant
activity of silymarin. Journal of Enzyme Inhibition and Medicinal Chemistry. 24: 395-405. doi: 10.1080/14756360802188081
Oyaizu, M., 1986. Studies on product of browning reaction prepared from glucose amine. Japanese Journal of Nutrition. 44: 307-315. doi:10.5264/eiyogakuzashi.44.307
Pieroni, A., Janiak, V., Durr, C.M., Ludeke, S., Trachsel, E., Heincrich, M., 2002. In vitro antioxidant activity of non-cultivated vegetables of ethnic Albanians in Southern Italy. Phytotherapy Reseach. 16: 467-473. doi: 10.1002/ptr.1243
Pietta, P., Sionetti, P., Mauri, P., 1998. Antioxidant activity of selected medicinal plants. Journal of Agricultural and Food Chemistry. 46: 4487- 4490. doi: 10.1021/jf980310p Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C.,
1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology & Medicine. 26: 1231-1237. doi: 10.1016/S0891-5849(98)00315-3
Shahidi, F., Janitha, P.K., Wanasundara, P.D., 1992. Phenolic antioxidants. Critical Reviews in Food Science and Nutrition. 32: 67-103. doi: 10.1080/10408399209527581
Singleton, V.L., Orthofer, R., Lamuela-Raventos, R.M., 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology. 299: 152-178. doi: 10.1016/S0076-6879(99)99017-1