Sevil YÜCEL, Pınar TERZİOĞLU, Zeynep AYDIN SİNİRLİOĞLU, Bilge Sema TEKEREK, Yeliz BAŞARAN ELALMIŞ

SYNTHESIS, CHARACTERIZATION, IN VITRO DEGRADABILITY AND BIOACTIVITY OF STRONTIUM SUBSTITUTED RICE HULL ASH SILICA BASED MELT DERIVED 45S5 BIOACTIVE GLASS

The purpose of this study was to synthesize strontium substituted melt derived 45S5 bioactive glasses by use of rice hull ash silica and commercial silica. The degradation behaviour of bioactive glass samples was studied through the weight loss in the tris-(hydroxymethyl)-aminomethane solution. The in vitro bioactivity of the bioactive glass samples was evaluated by soaking studies in simulated body fluid. The apatite like layer formation on glasses surface was assessed by X-ray powder diffraction, scanning electron microscope, inductively coupled plasma and Vickers hardness measurement analyses. Also, the effect of bioactive glass samples on osteoblasts in vitro and cell mineralisation was investigated. The present investigation revealed that both of the samples show similar bioactivity but 45S5 bioactive glass from rice hull ash silica supported cell activity better than commercial silica. Thus, rice hull ash silica can be used to synthesize strontium substituted 45S5 bioactive glasses and it is also good alternative to commercial silica.

Keywords:

Bioactive glass, rice hull ash silica, strontium.,

PDF

___

[1] Gentleman E., Fredholm Y.C., Jell G., et.al., “The effects of strontium-substituted bioactive glasses on osteoblasts and osteoclasts in vitro”, Biomaterials, 31, 3949–3956, 2010.
[2] Tsigkou O., Jones J.R., Polak J.M., et.al., “Differentiation of fetal osteoblasts and formation of mineralized bone nodules by 45S5 bioglass conditioned medium in the absence of osteogenic supplements”, Biomaterials, 30,21, 3542–3550,2009.
[3] Hench L.L., Thompson I., “Twenty-first century challenges for Biomaterials”, J. R. Soc. Interface, 7, 379–391, 2010.
[4] Goel A., Rajagopal R.R., Ferreira J.M.F., “Influence of strontium on structure, sinteringandbiodegradationbehaviourof CaO–MgO–SrO–SiO2–P2O5–CaF2 glasses”, ActaBiomaterialia, 7, 4071–4080, 2011.
[5] Usuda K., Kono K., Dote T., et.al., “An overview of boron, lithium, andstrontium in humanhealth andprofiles of theseelements in urine of Japanese”, EnvironHealthPrevMed, 12, 6, 231–237, 2007.
[6] Fredholm Y.C., Karpukhina N., Law R.V., et.al., “Strontium containing bioactive glasses: Glass structure and physical properties”, J Non-Cryst Solids, 356,2546–2551, 2010.
[7] Oudadesse H., Dietrich E., Bui X.V., et.al., “Enhancement of cells proliferation and control of bioactivity of strontium doped glass”, Applied Surface Science, 257, 8587– 8593, 2011.
[8] Gorustovich A.A., Steimetz T., Cabrini R.L., et.al., “Osteoconductivity of strontium-doped bioactive glass particles: a histomorphometric study in rats”, J Biomed Mater Res A, 92A, 1, 232-237, 2010.
[9] Yucel S., Özçimen D., Terzioğlu P., et.al., “Preparation of Melt Derived 45S5 Bioactive Glass from Rice Hull Ash and Its Characterization”, Adv. Sci. Lett., 19, 3477-3481, 2013.
[10] Kalapathy U., Proctor A., Shultz J. , “A simple method for production of pure silica from rice husk ash”, Bioresource Technol. ,73, 257-262, 2000.
[11] Taş, A.C. , “Synthesis of Biomimetic Ca-Hydroxyapatite Powders at 37°C in Synyhetic Body Fluids”, Biomaterials, 21, 1429-1438, 2000.
[12] Jones J.R., Gentleman E., Polak J., “Bioactive glass scaffolds for bone regeneration”, Elements, 3,6,393–399,2007.
[13] Montjovent M.O., Burri N., Mark S., et.al., “Fetal bone cells for tissue engineering”, Bone, 35, 1323–1333, 2004.