Seseli tortuosum’un Antioksidan Aktivitesi ve Yağ Asidi Kompozisyonu

Birçok çalışma oksidatif stresle ilişkili çeşitli hastalıkların engellenmesinin yanı sıra insan sağlığına bitki sekonder metabolitlerinden kaynaklanan faydaları göstermiştir. Bu çalışmada Seseli tortuosum’un antioksidan aktivitesi serbest radikal süpürme (DPPH), indirgeme gücü (FRAP ve CUPRAC), toplam antioksidan kapasite (β-karoten/linoleik asit ve fosfomolibdat test) içeren farklı kimyasal metotlar tarafından araştırıldı. Ayrıca, toplam fenolik içerik Folin-Ciocalteu testi tarafından tespit edildi. BHA, BHT ve Troloks bu antioksidan testlerde standart olarak kullanıldı. S. tortuosum’un yağ asiti kompozisyonu Gaz Kromatografisi (GC) tarafından değerlendirildi. Özütte toplam fenolik içerik 36.58 mgGAE/g özüt olarak tespit edildi. Özüt antioksidan deneylerde konsantrasyona bağlı olarak antioksidan aktiviteye sahiptir. S. tortuosum yağında 24 yağ asiti tanımlandı. Yağ asitleri arasında, linoleik asit C 18:2 ω6 (%34.57) ana bileşendir. Doymamış yağ asitlerinin miktarı doymuş yağ asitlerinden daha yüksektir. Bu sonuçlardan,  S. tortuosum yeni gıda ve ilaç ürünlerinin tasarlanması için değerli bir kaynak olarak düşünülebilir.

Antioxidant Activity and Fatty Acid Composition of Seseli tortuosum

 Many studies have shown the benefits deriving from plants secondary metabolites to human health also for the prevention of several diseases associated to oxidative stress. In this work, the antioxidant activity of Seseli tortuosum was investigated by different chemical methods including free radical scavenging (DPPH assay), reducing power (FRAP and CUPRAC assays), total antioxidant capacity (β-caroten/linoleic acid and phosphomolybdate assays). Also, total phenolic content were detected by Folin-Ciocalteu assay. BHA, BHT and Trolox were used as standards in these antioxidant assays. The fatty acid composition of S. tortuosum was also evaluated by gas chromatography (GC). Total phenolic content in the extract was detected as 36.58 mgGAE/g extract. The extract possesses antioxidant activity in a concentration-dependent manner at the antioxidant assays. Twenty-four fatty acids were identified in the S. tortuosum oil. Among fatty acids, the main component was linoleic acid (C 18:2 ω6) (34.57%). The content in unsaturated fatty acids (UFAs) was higher than that of saturated fatty acids (SFAs). From these results, S. tortuosum could be considered as a valuable source for designing novel food and pharmaceutical products.

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