An experiment was conducted to determine the effect of enzyme supplementation to laying hens diets containing different levels of sunflower seed meal on performance and egg quality. The experiment was used 128 laying hens (HyLine W36) at 44 week of age throughout 84 days. Sixteen diets consisting of four levels of sunflower seed meal (0, 5, 10 and 20 % diet) and four levels enzyme (0, 500, 1000 and 2000 mg/ kg diet) in 4 x 4 factorial arrangement were used with four replicates of two birds each. During the experiment, feed and water were used as ad libitum. The effect of enzyme supplementation to laying hens diets containing different levels of sunflower seed meal did not significantly effect on egg production, egg weight, egg mass, feed intake, feed conversion ratio, egg shell weight, egg shell thickness and egg shell breaking strength (P>0.05). The result of this study that containing 20% sunflower seed meal to laying hens diets can be used without adversely affecting performance and egg quality.