The aim of this study was to determine the breed and carcass regions according to fatty acids in lambs by using discriminant analysis. In the study, saturated fatty acid (SFA), monounsaturated fatty acid (MUFA), polyunsaturated fatty acid (PUFA), trans fatty acid, conjugated linoleic acid (CLA), omega 3 (ω 3), omega 6 (ω 6), palmitic acid (C16: 0), margaric acid (C17: 0), stearic acid (C18: 0) and oleic acid (C18: 1 ω9) of 47 male lambs belonging to 5 different sheep breeds (Akkaraman, Dağlıç, Kıvırcık, Malya and Karacabey Merino) were used. With the discriminant analysis method, whether sheep breeds and carcass region (leg, shoulder, rib, and breast) could be classified correctly or not was investigated. At the end of the study, it was determined that when fatty acids were used, sheep breeds could be classified correctly in 57.3% and carcass regions in 70.2%. According to the results obtained, it was seen that the fatty acids resolved according to both sheep breeds and carcass regions. In this way, it can be said that by looking at the fatty acids content of the meat sample taken from any place, clues can be obtained about which sheep breed or which carcass region it might belong to.