Comparison of Antibacterial Effects of Pulp Capping Materials

Background: The aim of this study is to evaluate the antibacterial effects of six different pulp capping materials on Streptococcus mutans, Lactobacillus acidophilus and Enterococcus faecalis bacteria in different time periods. Methods: In our study, the antibacterial effects of TheraCal LC (BISCO Inc., USA), Dycal (Dentsply Tulsa Dental, USA), Biodentine (Septodont, France), Calcimol LC (Voco GmbH, Germany), Activa (Pulpdent Corporation, USA) and MTA Angelus (Angelus, Brazil) were tested against strains of S. mutans (ATCC 25175), L. acidophilus (RSKK 03037) and E. faecalis (ATCC 29212) using the disk diffusion method. Each of the materials was hardened in standard, 5 mm diameter, hollow sterile discs prepared beforehand in line with the manufacturer's recommendations. The materials extracted from the discs were placed in sterile sample containers. The materials placed in the sample containers were disinfected under UV light device for 20 minutes. The materials were placed in petri dishes containing the study isolates that were inoculated into the media at 2.5-3 cm intervals and homogeneously distributed. The same procedures were repeated for each microorganism in 10 separate petri dishes. The inhibition zone diameters around the discs that were left to incubate for 24-48 hours at 37°C were measured in millimeters. Statistical analysis of the data was performed using one-way analysis of variance (One Way ANOVA), Kolmogorov- Smirnow, Levene tests and Bonferroni tests. Results: At the end of the study, the tested materials showed a statistically significant antibacterial effect on all bacterial strains used (p 0.05). Conclusion: We think that the antibacterial effect of pulp capping agents against S. mutans L. acidophilus E. faecalis bacteria, which is involved in the formation and development mechanism of caries, will contribute to the preservation of pulp vitality

Pulpa Kaplama Materyallerinin Antibakteriyel Etkilerinin Karşılaştırılması

Amaç: Bu çalışmanın amacı, altı farklı pulpa kaplama materyalinin, farklı zaman dilimlerinde Streptococcus mutans, Lactobacillus acidophilus ve Enterococcus faecalis bakterileri üzerindeki antibakteriyel etkilerini değerlendirmektir. Gereç ve Yöntemler: Çalışmamızda TheraCal LC (BISCO Inc., ABD) Dycal (Dentsply Tulsa Dental, ABD) Biodentine (Septodont, Fransa) Calcimol LC (Voco GmbH, Almanya) Activa (Pulpdent Corporation, ABD) ve MTA Angelus (Angelus.Brazil) materyallerinin antibakteriyel etkileri, disk difüzyon yöntemi ile S. mutans (ATCC 25175) L. acidophilus (RSKK 03037) ve E. faecalis (ATCC 29212) suşları kullanılarak değerlendirildi. Materyallerin her biri, üreticinin önerileri doğrultusunda önceden hazırlanmış standart 5 mm çapında içi boş steril disklerde sertleştirildi. Disklerden çıkarılan materyaller steril numune kaplarına yerleştirildi. Numune kaplarına konulan malzemeler UV ışık cihazı altında 20 dakika süreyle dezenfekte edilerek, 2.5-3 cm aralıklarla inokule edilen ve homojen olarak dağıtılan çalışma izolatlarını içeren petri kaplarına yerleştirildi. Aynı işlemler 10 ayrı petri kabında her bir mikroorganizma için tekrarlandı. 24-48 saat 37°C'de inkübasyona bırakılan disklerin etrafındaki inhibisyon zon çapları milimetre olarak ölçüldü. Verilerin istatistiksel analizi, tek yönlü varyans analizi (One Way ANOVA), Kolmogorov-Smirnow Levene testleri ve Bonferroni testleri kullanılarak yapıldı. Bulgular: Çalışmanın sonunda, test edilen materyaller, kullanılan tüm bakteri suşları üzerinde istatistiksel olarak anlamlı bir antibakteriyel etki gösterdi (p 0.05). Sonuç: Çürüklerin oluşum ve gelişme mekanizmasında yer alan S. mutans, L. acidophilus, E. faecalis bakterilerine karşı pulpa kaplama ajanlarının antibakteriyel etkili göstermesinin pulpa canlılığının korunmasına katkı sağlayacağını düşünmekteyiz.

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