SUBARAKNOİD KANAMANIN NEDEN OLDUĞU SEREBELLAR HASARDA UYGULANAN FARKLI TEDAVİ MODALİTELERİNİN OKSİDATİF STRES PARAMETRELERİ ÜZERİNE ETKİLERİ

Amaç Bu çalışmada, Subaraknoid Kanama (SAK) sonrasında serebellar dokuda meydana gelen oksidatif stres üzerinde, uygulanabilecek farklı tedavi modalitelerinin etkilerinin değerlendirilmesi amaçlanmıştır. Gereç-ve Yöntem Kuyruk arterlerinden alınan 0,3 cc otolog kanın sisterna magna bölgesine enjekte edilmesi ile SAK oluşturulan ratlara farklı dozlarda (30-60 mg/kg Pregabalin (PREG), Nimodipin (NİMO), Salubrinal (SLB) ve Darbeli Elektromanyetik Alan (DEMA) uygulandı. Sakrifikasyon sonrasında alınan serebellum dokularında total oksidan kapasite (TOS), total antioksidan kapasite (TAS) ve oksidatif stres indeksi (OSİ) gibi oksidatif stres parametreleri incelendi. Bulgular TOS ve OSİ değerlerinde PREG30 (iki değer için de p=0.003), PREG₆₀ (p=0.026 ve p=0.005, sırasıyla), SLB ve DEMA uygulanan gruplarda anlamlı olarak azalma görüldü. TAS seviyelerinde ise PREG ve DEMA uygulanan gruplarda istatistiksel olarak anlamlı bir değişiklik saptanmazken, SLB ve NİMO uygulanan gruplarda anlamlı değişiklik olduğu görüldü. NİMO uygulanan grupta OSİ değerinde de (p=0,046) SAK grubuna göre anlamlı bir azalma saptandı. Sonuç PREG ve SLB SAK’ta farklı iki mekanizma üzerinden koruyucu olabilmektedir. DEMA tedavisinin de etkinliğinden söz edilebilir ancak etkisinin değerlendirilmesi için farklı uygulama zamanları ve süreleri ile yeni çalışmalara ihtiyaç vardır.

Anahtar Kelimeler:

subaraknoid kanama, oksidatif stres, pregabalin, DEMA, sıçan, serebellum

THE EFFECTS OF DIFFERENT TREATMENT MODALITIES ON OXIDATIVE STRESS MARKERS FOR CEREBELLAR INJURY SECONDARY TO SUBARACHNOID HEMORRHAGE

Objective In this study, we aimed to evaluate the effects of different treatment modalities on oxidative stress occured in cerebellar tissue after subarachnoid hemorrhage (SAH). Materials and Methods Different doses of Pregabaline (30-60 mg/kg Pregabalin (PREG), and Nimodipine (NIMO), Salubrinal (SLB), Pulsed ElectromagneticFfield (PEMF) was used in the rats, that SAH was comprised via 0,3cc otolog blood injection into the cisterna magna. After sacrification oxidative stress parameters like Total Antioxidant Status (TAS), Total Oxidant Status (TOS), and Oxidative Stress Index (OSI) were evaluated in the cerellum tissues. Results Significant decrease was seen in the TOS and OSI values of the groups that PREG30 (p=0.003 for both), PREG₆₀ (p=0.026 and p=0.005, respectively), SLB and PEMF were used. Although there was no statistically difference in the TAS values of the PREG and DEMA groups, significant difference was seen in the SLB and NIMO groups. In the NIMO group there was a significant decrease (p=0,046) in the OSI values according to the SAH group. Conclusion PREG and SLB can be protective in SAH via two different mechanisms, PEMF treatment can be effective but new studies with different application periods and doses are needed to evaluate the effects.

Keywords:

subarachnoid hemorrhage, oxidative stress, pregabaline, PEMF, rat, cerebellum,

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