FARE GEBELİK DÖNEMİNDE HAREKETSİZLİK STRESİNİN PLASENTA VE YAVRUYA ETKİLERİ

ÖZ AMAÇ: Stres gebelik sürecinde anne ve fetüs sağlığını olumsuz etkilemektedir. Çalışmamızda kronik hareketsizlik stresinin plasenta ve fetüs gelişimi üzerine etkilerini araştırmayı hedefledik. GEREÇ VE YÖNTEMLER: Balb/c suşu dişi fareler (20-30gr), 2dişi-1erkek olacak şekilde katıma alındı. Kontrol grubundaki (n=6) gebe farelere herhangi bir uygulama yapılmazken, stres grubundaki (n=6) gebe farelere gebeliğin 6.gününden 18.gününe kadar günde 3 defa 45dakikalık kronik hareketsizlik stresine maruz bırakıldı. Gebeliğinin 18. Gününde plasenta ve fetüsler anestezi altında sezaryen ile alındı. BULGULAR: Prenatal stres, trofoblastik dev hücreler, glikojen içeren hücreler ve labirent trofoblastik hücreler dahil olmak üzere birçok plasental hücrede apoptozu önemli ölçüde arttırdı ve intrauterin büyüme geriliğine sebep oldu. Stres süperoksit dismutaz ve glutatyon seviyelerini azalttı. Fetüsün gelişimini değerlendirmek için, Alizarin Red S boyaması ile fetüsün kemikleşme merkezleri değerlendirildi. SONUÇ: Gebelik sürecindeki stres, apoptozu tetikleyerek, labirent bölgesi küçüldü ve plasenta yetmezliğine sebep oldu, ayrıca kollajen seviyelerini arttırarak fetüs gelişimini olumsuz yönde etkileyerek intrauterin büyüme geriliği patogenezinde katkısı olduğunu gözlemledik.

Anahtar Kelimeler:

Fetus, hareketsizlik stresi, plasenta

THE EFFECTS OF IMMOBILIZATION STRESS ON PLACENTA AND FETUS IN PREGNANT MICE

ABSTRACT AIM: Stress can affect negatively mother and fetuses during pregnancy. We aimed to investigate the effects of chronic immobilization stress on placental maturation and fetal development.MATERİALS AND METHODS: Balb/c virgin female mice (20-30 g) were mated with male mice in a 2 to 1 female to male ratio. Pregnant mice in control group (n=6) were left undisturbed, whereas pregnant mice in the stress group (n=6) were exposed to 45 min chronic immobilization stress for three times/day starting from gestational day 6 till 18. Fetuses and placentas were removed from dams on the gestational day 18 under anesthesia.RESULTS: The prenatal stress significantly increased apoptosis in several placental cells including trophoblastic giant cells, glycogen cells and labyrinth trophoblastic cells and resulted in intrauterine growth restriction. The stress caused a decreased superoxide dismutase and glutathione levels. Alizarin Red S staining shows the ossification center of the fetuses to see developmental abnormality. CONCLUSION: Gestational stress causes placental dysfunctions by triggering apoptosis, reducing the labyrinth zone as well as increasing collagen levels, which may impair fetal development that may contribute to pathogenesis of intrauterine growth restriction.

Keywords:

fetus, immobilization stress, placenta,

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