DENEYSEL BÖBREK İSKEMİ REPERFÜZYON HASARINDA FARKLI DOZLARDAKİ OMEGA 3 YAĞ ASİTLERİNİN KORUYUCU ETKİLERİNİN ARAŞTIRILMASI

Amaç İskemi reperfüzyon (IR) hasarı, belirli bir organa kan akışının ani ve geçici olarak bozulmasından kaynaklanır. Renal IR kaynaklı böbrek hasarı, yüksek düzeyde kalıcı hasar ve mortaliteye neden olur. Diyet ile alınan omega-3 yağ asitleri, antiinflamatuar ve immün modüle edici etkilere sahiptir ve koruyucu olarak kullanılmaktadır. Çalışmada; iskemi reperfüzyon hasarında omega-3 yağ asidinin farklı dozlarının koruyucu etkileri biyokimyasal ve histolojik analizler ile incelenmiştir. Gereç ve Yöntem 40 adet erişkin dişi sıçan Düz kontrol, sham kontrol, IR, IR+100, IR+400, IR+700 olmak üzere 5 gruba ayrıldı (n:8). Sham grubunda sağ böbrek nefrektomi ile alındı, sol böbreğe işlem uygulanmadı. I/R hasarına maruz kalan sağ böbrek nefrektomi ile alındı, sol böbreklere ise klemp takıldı. Tedavi gruplarına cerrahi girişimden önceki 14 gün ve operasyon gününün sabahında toplam 15 sefer 100 mg/kg, 400 mg/kg ve 700 mg/kg’lık omega-3 yağ asitleri gastrik gavaj ile verildi. Bulgular Elde edilen verilere göre; artan dozlardaki omega-3 ile histopatolojik inceleme sonuçları korale şekilde iyileşme tespit edildi. Histopatolojik sonuçlar, biyokimyasal sonuçlar ile desteklendi. Malondaildehit (MDA) ve myeloperoksidaz (MPO) düzeylerinde tedavi uygulanan gruplarda anlamlı düzeyde azalma saptanırken, katalaz (CAT) ve glutatyon peroksidaz (GSH-Px) düzeylerinde artış saptandı. Sonuç Sonuç olarak; elde edilen bulgulara göre, böbrekte iskemi ve reperfüzyon hasarı oluşturma ihtimali olan vakalarda önceden omega-3 yağ asidi verilmesinin bu dokuda koruyucu etki sağlayabileceği sonucuna ulaşılmıştır.

Anahtar Kelimeler:

Böbrek, İnflamasyon, İskemi-Reperfüzyon Hasarı, Oksidatif stres, Omega 3 yağ asitleri

INVESTIGATION OF PROTECTIVE EFFECTS OF DIFFERENT DOSES OF OMEGA 3 FATTY ACIDS IN EXPERIMENTAL RENAL ISCHEMIA REPERFUSION DAMAGE

Objective Ischemia reperfusion (IR) injury is caused by a sudden and temporary impairment of blood flow to a particular organ or tissue. Renal IR-induced kidney damage causes high levels of permanent damage and mortality. Dietary omega-3 fatty acids have anti-inflammatory and immune-modulating effects and are used as protective agents. Materials and Methods In the study; Protective effects of different doses of omega-3 fatty acid in ischemia reperfusion injury were investigated by biochemical and histological analyzes. 40 adult female rats were divided into 5 groups as sham control, IR, IR+100, IR+400, IR+700 (n:8). The right kidney was removed by nephrectomy in the sham control group, and the left kidney was not treated. For IR injury; the right kidney was removed by nephrectomy, and the left kidney was clamped. Omega- 3 fatty acids of 100 mg/kg, 400 mg/kg and 700 mg/ kg were administered by gastric gavage to the treatment groups for 14 days before the surgery and for 15. gastric gavage was administrated in the morning of the operation. Results According these data; with application of omega-3, histopathological examination results were correlated. It is found that, histopathological results were supported by biochemical results. There was a significant decrease in malondialdehyde (MDA) and myeloperoxidase (MPO) levels in the treated groups, while catalase (CAT) and glutathione peroxidase (GSH-Px) levels were detected to be increased. Conclusion It was concluded that administration of omega-3 fatty acids beforehand in cases that may cause ischemia and reperfusion damage in the kidney can provide a protective effect on this tissue.

Keywords:

Inflammation, Ischemia-Reperfusion Injury, Kidney, Omega 3 fatty acids, Oxidative stress,

PDF

___

1. Jang HR, Rabb H. The innate immune response in ischemic acute kidney injury. Clin Immunol. 2009;130:41-50.
2. Ergün O, Ulman C, Kılıçalp AS. Carnitine as a agent in experimental renal ischemia-reperfusion injury. UroI Res 2001;29:186-9.
3. Guyton AC. The kidneys and body fluids. In: Textbook of Medical Physiology. 9th edition. Philadelphia: WB Saunders Company; 1996. 297–313.
4. Gasanov, F., Aytac, B., & Vuruskan, H. (2011). The effects of tadalafil on renal ischemia reperfusion injury: an experimental study. Bosnian journal of basic medical sciences, 11(3), 158.
5. Malek, M., & Nematbakhsh, M. (2015). Renal ischemia/reperfusion injury; from pathophysiology to treatment. Journal of renal injury prevention, 4(2), 20.
6. Islam CF, Mathie RT, Dinneen MD, Kiely EA, Peters AM, Grace PA. Ischemia–reperfusion injury in the rat kidney the effect of preconditioning. Br J Urol 1997;6:842–7.
7. Calder, P. C., & Yaqoob, P. Understanding omega-3 polyunsaturated fatty acids. Postgraduate medicine, 121(6), 148-157.Calder PC: Polyunsaturated fatty acids, inflammation, and immunity. Lipids 2001, 36:1007–1024.
8. Mori TA, Beilin LJ: n-3 Fatty acids, blood lipids and cardiovascular risk reduction. Curr Opin Lipidol 2001, 12:1211–1217.
9. Alejandro V, Scandling JD, Sibley RK, et al: Mechanisms of filtration failure during postischemic injury of the human kidney. J Clin invest 95: 820-831,1995
10. Simmons MN, Schreiber MJ, Gill IS. Surgical Renal Ischemia: A Contemporary Overview. J Urol. 2008;180(1):19-30.
11. Fouad AA, Al-Mulhim AS, Jresat I, Morsy MA. Protective effects of captopril in diabetic rats exposed to ischemia/reperfusion renal injury. J Pharm Pharmacol. 2013;65(2):243- 252.
12. Kocoglu H, Ozturk H, Ozturk H, et al. Effect of dexmedetomidine on ischemiareperfusion injury in rat kidney: A histopathologic study. Ren Fail. 2009;31(1):70-74.
13. Williams P, Lopez H, Britt D, et al. Characterization of Renal Ischemia-Reperfusion Injury in Rats. J Pharmacol Toxicol Methods. 1997;Feb:37(1):1-7
14. Mortensen M, Rosenfield RL, Littlejohn E. Functional significance of polycysticsize ovaries in healthy adolescents. J Clin Endocrinol Metab, 2006, 91: 3786-3790.
15. Sarsılmaz M, Songur A, Özyurt H, et al. Potential role of dietary ω-3 essential fatty acids on some oxidant/antioxidant parameters in rats’ corpus striatum. Prostagl Leukot Essent Fatty Acids 2003; 69(4): 253-259.
16. Miyasaca CK, Alves de Souza JA, Torres RP, Filho JM, Lajolo FM, Curi R. Effect of the administration of fish oil by gavage on activities of antioxidant enzymes of rat lympohoid organs. Gen Pharmacol 1998; 30 (5): 759-762.
17. Grande JP, Walker HJ, Holub BJ ve ark. Suppressive effects of fish oil on mesengial cell proliferation in vitro and in vivo.Kidney Int 2000;57:1027-1040
18. Slater TF. Free radical mechanisms in tissue injury. J Biochem 1984; 222:1-15.
19. Ozan E. Koyutürk L.Sapmaz T. Böbrek İskemi Reperfüzyon Hasarında Antioksidan Olarak Prostoglandin E1(PGE)Kullnımının İncelenmesi: Fırat Üniversitesi Tıp Fakültesi Histoloji Ve Embriyoloji Anabilimdalı. Fırat Tıp Dergisi 2004;9(3):67-71
20. Ohkawa H, Ohishi N, Tagi K.Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979; 95: 351-358.
21. İnal M, Altınışık M, Bilgin MD. The effect of quercetin on renal ischemia and reperfusion injury in the rat. Cell Biochem Funct. 2002 dec; 20(4):291-6.
22. Wall J. Antioxidants In Prevention Of Reperfusion Damage Of Vascular Endothelium. TSMJ May 2000;1:67-70.
23. Becker BF, Reinholz N, Leipert B, Raschke P, Permanetter B, Gerlach E. Role of uric acid as an endogenous radical scavenger and antioxidant. Chest. 1991; 100: 176-181.