Nasıf Fatih KARAKUYU, Halil AŞCI, Mehtap SAVRAN, Kanat GÜLLE

AKRİLAMİDE MARUZ KALAN SIÇANLARIN KALP DOKUSU ÜZERİNDE SELENYUMUN KORUYUCU ROLÜ

Amaç Akrilamid (ACR), farklı endüstriyel alanlarda yaygın olarak kullanılan zararlı organik reaktif bir bileşiktir. Selenyum (SEL), hem hayvanlarda hem de insan organizmalarında meydana gelen bazı kimyasal ve enzimatik reaksiyonlarda yer alan ve eksikliği durumunda kardiyovasküler sistem gibi birçok sistemde bazı rahatsızlıklara neden olabilen bir eser elementtir. Bu çalışmanın amacı, sıçanlarda deneysel olarak oluşturulan ACR toksisitesinde SEL'in kalp dokusu üzerinde koruyucu bir etkisinin olup olmadığını araştırmaktır. Gereç ve Yöntem Toplam 28 sıçan rastgele ve eşit olarak dört gruba ayrıldı: Kontrol, SEL, ACR, SEL + ACR. Çalışma sonunda kan örneklerinden kreatin kinaz MB (CKMB), laktat dehidrojenaz (LDH) ve iskemi modifiye albümin (IMA) gibi kardiyak belirteçler ile kalp dokusundan toplam oksidan durumu (TOS), toplam antioksidan durumu (TAS) ve oksidatif stres indeksi (OSI) gibi oksidatif stres belirteçlerinin analizleri yapıldı. Ayıca kalp kesitlerinde immünohistokimyasal yöntemler kullanılarak indüklenebilir nitrik oksit sentaz (iNOS) aktiviteleri belirlendi. Bulgular ACR + SEL grubunda, ACR grubuna göre TOS, OSI, CKMB, LDH, IMA düzeylerinde anlamlı düşüş ve TAS düzeyinde önemli artış gözlemlendi. Kalp dokusu kesitlerinin histopatolojik ve immünhistokimyasal değerlendirmesinde; ACR grubunda kalp kası liflerinde düzensizlik, kas lifleri arasında bağ dokusu artışı ve kas liflerinde düzensiz eozinofili tespit edildi. SEL uygulanan gruplarda ACR grubuna göre histopatolojik bulgularda azalma gözlendi. iNOS immünreaktiviteleri; ACR grubunda orta düzeyde ekspresyon gözlenirken, ACR + SEL grubunda daha zayıf bir ekspresyon gözlendi. Sonuç ACR'nin sıçanların kalp dokusunda çeşitli metabolik yollarla hasara yol açtığını ve bu hasarın SEL verilen gruplarda tersine döndürüldüğünü belirledik.

Anahtar Kelimeler:

Selenyum, akrilamid, antioksidan, sıçan

PROTECTIVE ROLE OF SELENIUM ON THE HEART OF RATS EXPOSED ACRYLAMIDE

Objective Acrylamide (ACR) is a harmful organic reactive compound widely used in different industrial fields. Sele- nium (SEL) is a trace element that takes part in some chemical and enzymatic reactions in both animals and human organisms and can cause some disorders in many systems such as the cardiovascular system in case of deficiency. The aim of this study is to investigate whether SEL has a protective effect on cardiac tissue in experimentally induced ACR toxicity in rats. Material and Method A total of 28 rats were randomly and equally divided into four groups: Control, SEL, ACR, SEL + ACR. At the end of the study, cardiac markers such as creatine kinase MB (CKMB), lactate dehydrogenase (LDH) and ischemia-modified albumin (IMA) from blood samples were measured. Oxidative stress markers such as total oxidant status (TOS), total antioxidant status (TAS) and oxidative stress index (OSI) in heart tissue were analyzed. In addition, inducible nitric oxide synthase iNOS activities were determined in heart sections using immunohistochemical methods. Results The ACR + SEL group showed a significant decrease in TOS, OSI, CKMB, LDH, IMA levels and significant increase in TAS level compared to the ACR group. In histopathological and immunohistochemical evaluation of the heart sections; Disorganization in cardiac muscle fibers, increased connective tissue between muscle fibers and irregular eosinophilia in muscle fibers were detected in ACR group. A decrease in histopathological findings was observed in the SEL treated groups compared to the ACR group. iNOS immunoreactivities; moderate marking was observed in the ACR group sections, while poor marking was observed in the ACR + SEL group. Conclusion We determined that ACR caused damage to the heart tissue of rats through various metabolic pathways and that these damages were reversed in the groups given the SEL.

Keywords:

Selenium, acrylamide, antioxidant, rat,

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