BUĞDAY ÇİMİNİN İNSAN LENFOSİT HÜCRELERİ ÜZERİNE ETKİSİ

Amaç Kemoterapötik ilaçlar kanser hücrelerinin ortadan kaldırılmasında etkili iken aynı zamanda sağlıklı hücrelerde de hasar oluşturabilmektedir. Bu çalışmada, buğday çimi (Triticum aestivum L.) ekstraktının fenolik bileşen içeriğinin analizi ve bu ekstraktın kemoterapötik tedavide kullanılan sisplatin ve etoposid’in sağlıklı hücrelerde oluşturduğu DNA hasarına karşı etkisinin belirlenmesi amaçlanmıştır. Gereç ve Yöntem Çimlendirilmiş buğdayların metanol ekstraktı hazırlanarak HPLC (yüksek performanslı sıvı kromatografisi) ile fenolik bileşen analizi yapıldı. Buğday ekstraktı konsantrasyonuna bağlı hücre canlılık testi uygulanarak IC50 (Yarı maksimum inhibitör konsantrasyonu) ve LD50 (ortalama öldürücü doz) değerleri hesaplandı. Belirlenen bu konsantrasyon değerleri ile hücreler inkübe edilerek DNA hasarı varlığı Comet metodu ile değerlendirildi. Bulgular Fenolik bileşen analizi sonucunda p-hidroksibenzoik asit en yüksek miktarda, o-kumarik asit ise en düşük düzeyde tespit edildi. Lenfosit hücrelerine uygulanan farklı konsantrasyonlardaki buğday çimi ekstraktı, etoposid ve sisplatin için değerler sırasıyla IC50=204,6 μg/mL, LD50=15,84 μg/mL ve LD50=24,51 μg/mL olarak bulundu. Comet analizi sonucunda kontrol grubuna kıyasla, etoposid LD50 ve etoposid LD50+buğday çimi ekstraktı IC50 grubu istatistiksel olarak anlamlı bulunurken (p<0,05), etoposid LD50 ve etoposid LD50+buğday çimi ekstraktı IC50 grubu arasında istatistiksel olarak anlamlılık bulunamadı (p>0,05). Bu sonuca benzer olarak kontrol grubuna kıyasla, sisplatin LD50 ve sisplatin LD50+buğday çimi ekstraktı IC50 grubu istatistiksel olarak anlamlı bulunurken (p<0,05), etoposid LD50 ve etoposid LD50+buğday çimi ekstraktı IC50 grubu arasında istatistiksel olarak anlamlılık saptanmadı (p>0,05). Sonuç Çalışmamızda buğday çiminin etoposid ve sisplatin nedeni ile oluşan DNA hasarında azalmaya neden olduğu görülmüş olmasına rağmen istatistiksel olarak anlamlılık saptanmamıştır.

Anahtar Kelimeler:

Buğday çimi, Comet metodu, HPLC, MTT

EFFECT OF WHEATGRASS ON HUMAN LYMPHOCYTE CELLS

Objective While chemotherapeutic drugs are effective at eliminating cancer cells, they can also damage healthy cells. The aim of this study was to analyze the phenolic component content of wheatgrass extract (Triticum aestivum L.) and to determine the effect of this extract against DNA damage caused by cisplatin and etoposide used in chemotherapeutic treatment in healthy cells will. Material and Method Sprouted wheat methanol extract was prepared and analysis of phenolic components was performed by HPLC (high performance liquid chromatography). IC50 (half maximal inhibitory concentration) and LD50 (median lethal dose) values were calculated by applying a cell viability assay based on wheat extract concentration. Cells were incubated at these determined concentration levels and the presence of DNA damage was assessed by the Comet method. Results As a result of the phenol component analysis, p-hydroxybenzoic acid was determined in the highest amount and o-coumaric acid in the lowest amount. The values for wheatgrass extract, etoposide and cisplatin at different concentrations applied to lymphocyte cells were found to be IC50=204.6 μg/mL, LD50=15.84 μg/mL and LD50=24.51 μg/mL, respectively. As a result of comet analysis, it was found that etoposide LD50 and etoposide LD50+wheatgrass extract IC50 group were statistically significant (p<0.05), compared to the control group, there was no statistical significance between etoposide LD50 and etoposide LD50+wheatgrass extract IC50 group (p>0.05). Similar to this result, cisplatin LD50 and cisplatin LD50+wheatgrass extract IC50 group were found to be statistically significant compared to the control group (p<0.05), while there was no statistical significance between etoposide LD50 and etoposide LD50+wheatgrass extract IC50 group (p> 0.05). Conclusion Although wheatgrass was observed in our study to cause a reduction in DNA damage caused by etoposide and cisplatin, no statistical significance was found.

Keywords:

Wheatgrass, Comet method, HPLC, MTT,

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