Uğur ŞAHİN, Melda ŞAHİN, Okan SANCER, Mustafa CALAPOĞLU, Nurgül ŞENOL

ADİPONEKTİN’İN MCF-7 PROLİFERASYONU VE GİRELİN SEVİYESİ ÜZERİNE ETKİSİ

Amaç Lokal ve sistemik faktörlerin, östrojen reseptörü pozitif meme kanseri riskinde artış olan postmenopozal obez kadınlarda meme kanseri hücrelerinin büyümesini yönlendirdiği gösterilmiştir. Postmenapozal dönemdeki obez kadınlarda, dolaşımdaki girelin hormonunun azaldığı, bazı tümör hücreleri tarafından üretildiği ve ayrıca tümör büyümesini etkilediği gösterilmiştir. Bu çalışmada, adiponektin uygulamasının östrojen reseptörü pozitif MCF-7 meme kanseri hücre hattındaki rolünü ve girelin seviyeleri üzerine olan etkilerini araştırmayı amaçladık. Gereç ve Yöntem MCF-7 meme kanseri hücre hattı 2 boyutlu kültürde çoğaltıldı. MCF-7 meme kanseri hücrelerine artan konsantrasyonlarda adiponektin (50-5000 ng/mL) ile muamele edilerek inhibisyon dozu belirlendi. MCF-7 meme kanseri hücre hattına 50, 100, 250 ve 500 ng/ mL konsantrasyonda adiponektin uygulanarak 24, 48 ve 72. Saatlerde örneklerde girelin seviyeleri enzim bağlı immünosorbent analizi (ELISA) ile ölçüldü. Bulgular 24, 48 ve 72. saate alınan MCF-7 meme kanseri hücre hattı kültür örneklerinde 50, 100, 250 ve 500 ng/mL adiponektin konsantrasyonuna bağlı olarak proliferasyonun azaldığı bulundu. 24, 48 ve 72. saate alınan MCF-7 meme kanseri hücre hattı kültür örneklerinde 50, 100, 250 ve 500 ng/mL adiponektin konsantrasyonuna bağlı olarak girelin miktarında artış oluştuğu bulundu. Sonuç Çalışmamızda MCF-7 meme kanseri hücre hattında (ER/PR pozitif) anti-kanserojenik özelliği kanıtlanmış olan ve aynı zamanda obezite ile negatif korelasyon gösteren adiponektinin, girelin ile karşılıklı etkileşimlerinin, ER pozitif meme kanserlerindeki rolünü ortaya koymuş olduk.

Anahtar Kelimeler:

MCF-7, Adiponektin, Girelin, Hücre Kültürü, Meme Kanseri

EFFECT OF ADIPONECTIN ON MCF-7 PROLIFERATION AND GHRELIN LEVEL

Objective Local and systemic factors have been shown to drive the growth of breast cancer cells in postmenopausal obese women who are at increased risk of estrogen receptor-positive breast cancer. In obese postmenopausal women, it has been shown that circulating ghrelin hormone is decreased, produced by some tumor cells, and also affects tumor growth. In this study, we aimed to investigate the role of adiponectin administration in estrogen receptor positive MCF-7 breast cancer cell line and its effects on ghrelin levels. Material and Method The MCF-7 breast cancer cell line was propagated in 2D culture. The inhibition dose was determined by treating MCF-7 breast cancer cells with increasing concentrations of adiponectin (50-5000 ng/mL). Ghrelin levels were measured at the 24th, 48th ,and 72nd hours by enzyme-linked immunosorbent assay (ELISA) by administering adiponectin at a concentration of 50, 100, 250 and 500 ng/mL to the MCF-7 breast cancer cell line. Results In MCF-7 breast cancer cell line culture samples taken at the 24th, 48th and 72nd hours, proliferation was found to decrease depending on 50, 100, 250 and 500 ng/mL adiponectin concentration. It was found that the amount of ghrelin increased depending on 50, 100, 250 and 500 ng/mL adiponectin concentration in MCF-7 breast cancer cell line culture samples taken at 24th, 48th and 72nd hours. Conclusion In our study, we revealed the role of adiponectin, which has proven anti-carcinogenic properties in the MCF-7 breast cancer cell line (ER/PR positive) and also negatively correlated with obesity, and ghrelin, in ER-positive breast cancers.

Keywords:

Adiponectin, Ghrelin, Cell Culture, Breast Cancer, MCF-7,

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