Topraktan izole edilen Bacillus türlerinin tanımlanması ve biyolojik özelliklerinin araştırılması
Bu çalışmada, Giresun adasından toplanan toprak örneklerinden Bacillus izolasyonu yapılmıştır. Bu izolatlar morfolojik, biyokimyasal ve moleküler olarak tanımlanmıştır. Tanımlanan Bacillus'larda bazı ekstrasellüler enzimlerinin varlığı kalitatif olarak incelenmiştir. Ayrıca Bacillus izolatlarının bazı bakterilere karşı antibakteriyal aktiviteleri agar difüzyon metoduna göre araştırılmıştır. Sonuç olarak 38 izolat B. cereus grubu üyesi, 7 izolat B. thuringiensis, 10 izolat B. megaterium, 6 izolat B. pumilus ve 12 izolat Bacillus sp. olarak tanımlanmıştır. Bacillus izolatlarının ekstrasellüler enzim aktivite sonuçları değerlendirildiğinde 38 izolatın amilaz, 53 izolatın lipaz/esteraz, 16 izolatın kitinaz, 7 izolatın ksilanaz, 2 izolatın pektinaz, 73 izolatın proteaz ve 35 izolatın selülaz enzim aktivitesi pozitif olarak bulunmuştur. Çalışmada kullanılan Bacillus izolatları test edilen mikroorganizmaların gelişmelerini değişik oranlarda engellemiştir. Dokuz izolat yüksek antibakteriyal aktivite göstermiştir.
Identification of Bacillus species isolated from soil and investigation of their biological properties
In this study, Bacillus isolation were performed from soil samples collected from Giresun Island. These isolates were characterized as morphological, biochemical, and molecular. The presence of some extracellular enzymes in the identified Bacillus isolates were qualitatively investigated. Furthermore the antibacterial activities of the Bacillus isolates against some bacteria were examined by the agar diffusion method. As a result 38 isolates were identified as the members of B. cereus group, 7 as B. thuringiensis, 10 as B. megaterium, 6 as B. pumilus, and 12 as Bacillus sp. When the extracellular enzyme activity results of the identified Bacillus isolates were evaluated, it was found that 38 of these have amylase, 53 have lipase/esterase, 16 have chitinase, 7 have xylanase, 2 have pectinase, 73 have protease, and 35 have cellulase positive enzyme activities. The Bacillus isolates used in this study inhibited the growth of tested microorganisms in varying ratios. Nine isolates show high antibacterial activity
___
- J.A. Radley, "Production of Microbial Amylolytic Enzymes: Starch Production Technology", Editör : Underkofler, L.A. Applied Science Publisher Ld. England, 1976.
- S. Aira, K. Kilal ve A. Imanaka, "Cloning and Expression of Thermostable a-amylase Gene from Bacillus stearothermophilus in Bacillus stearothermophilus and Bacillus subtilis", Applied and Environmental Microbiology, sayı 46, pp. 1059-1065, 1983.
- T. O. Femi-Ola, ve I.I. Azeez, "Purification and Characterization of Beta-Amylase of Bacillus subtilis Isolated from Kolanut Weevil", Journal of Biology and Life Science, sayı 4, pp. 68-78, 2013.
- R.K. Saxena, A. Sheoran, B. Giri ve S.W. Davidson, "Purification Strategies for Microbial Lipases", Journal of Microbiological Methods, sayı 52, pp. 1-18, 2003.
- S. Ertuğrul, G. Dönmez ve S. Takaç, "Isoloation of Lipase Producing Bacillus sp. from Olive Mill Wastewater and Improving its Enzyme Activity", Journal of Hazardous Materials, sayı 149, pp. 720-724, 2007.
- J. Flach, P.E. Plet ve P. Jolles, "What's the New in Chitinase Research?", Experientia, sayı 48, pp. 701-716, 1992.
- W. K. Roberts ve C. P. Selitrennıkoff, "Plant and Bacterial Chitinases Differ in Antifungal Activity", Journal of General Microbiology, sayı 134, pp. 169-176, 1988.
- G.W. Goodey, "The Ecology of Chitin degradation", Advances in Microbial Ecology, sayı 11, pp. 387-430, 1990.
- R. Leah, H. Tommerup, I. Svendsen ve J. Mundy, "Biochemical and Molecular Characterisation Three barley Seed Proteins with Antifungal Properties", Journal of Biological Chemistry, sayı 266, pp. 1564-1573, 1995.
- B. L. Bassler, C. Yu, Y.C. Lee ve S. Roseman, "Chitin Utilization By Marine Bacteria: Degradation and Catabolism of Chitin Oligosaccarides By Vibrio Furnissi", Journal of Biological Chemistry, sayı 266, pp. 24276- 24286, 1991.
- J. Tomassen, A. Fıllloux, M. Bally, M. Murgıer ve A. Lazdundskı, "Protein Secretion İn Pseudomonas aeruginosa", FEMS Microbiology Reviews, sayı 103, pp. 73-90, 1992.
- K. Suzuki, M. Suzuki, M. Tayıyojı, N. Mıkaıdou ve T. Watanabe, "Chitin-binding protein (Cbp- 21) in the Culture Supernatant Serratia marsescens 2170", Bioscience, Biotechnology, and Biochemistry, 62, 128-135, 1998.
- V.W., Yang, Z., Zhuang, G. Elegir ve T.W. Jeffries, "Alkaline-actıve Xylanase Produced by an Alkaliphilic Bacillus sp. Isolated from Kraft Pulp", Journal of Industrial Microbiology and Biotechnology, sayı 15, pp. 434-441, 1995.
- B.C. Salles, R.B. Cunha, W. Fontes, M.V. Sousa ve E.X.F. Filho, "Purification and Characterization of a New Xylanase from Acrophialophora nainiana", Journal of Biotechnology, sayı 81, pp. 199-204, 2000.
- G. Gamerith, R. Groicher, S. Zeilinger, P. Herzog ve C.P. Kubicek, "Cellulase-Poor Xylanases Produced by Trichoderma reesei RUTC-30 on Hemicellulase Substrates", Applied Microbiology and Biotechnology, sayı 38, pp. 315-322, 1992.
- N. Roy ve H.M. Rowshanul, "Isolation and Characterization of Xylanase Producing Strain of Bacillus cereus from Soil", Microbiology, sayı 1, pp. 49-53, 2009.
- F. Yener, "Pektinaz Enziminin Farkli İki Destek Üzerine İmmobilizasyonu ve Karakterizasyonu", Yüksek lisans tezi. Çukurova Üniversitesi Fen Bilimleri Enstitüsü, Adana, 2007.
- D. J. Mukesh Kumar, G.M. Saranya, K. Suresh, P.D. Andal, R. Rajakumar ve P. T. Kalaichelvan, "Production and Optimization of Pectinase from Bacillus sp. MFW7 Using Cassava Waste", Asian Journal of Plant Science and Research, sayı 2, pp. 369-375, 2012.
- U.C. Banerjee, R.K. Sani, W. Azmi ve R. Soni, "Thermostable alkaline protease from Bacillus brevis and its Characterization as a Laundry Detergent Additive", Process Biochemistry, sayı 35, pp. 213-219, 1999.
- T.T. Teeri, A. Koivula, M. Linder, G. Wohlfahrt, C. Divne ve T.A. Jones, "Trichoderma reesei Cellobiohydrolases: Why so Efficent on Crystalline Cellulose", Biochemical Society Transactions, sayı 26, pp. 173-178, 1998.
- P. Tomme, R.A. Warren ve N.R. Gilkes, "Cellulose Hydrolis This Bacteria and Fungi", Advances in Microbial Physiology, sayı 37, pp. 1-81, 1995.
- S. Ito, "Alkaline Cellulases from Alkaliphilic Bacillus Enzymatic Properties, Genetics, and Application to Detergents", Extremophiles, sayı 1, pp. 61-66, 1997.
- C. Perez, C. Suarez, ve G.R. Castro, "Antimicrobial Activity Determined in Strains of Bacillus circulans Cluster", Folia Microbiology, sayı 38, pp. 25-28, 1993.
- F.A. Sharif ve N.G. Alaeddinoğlu, "A Rapid and Simple Method for Staining of the Crystal Protein of Bacillus thuringiensis", Journal of Industrial Microbiology, sayı 3, pp. 227-229, 1988.
- T. Beffa, M. Blanc, P.F. Lyon, G. Vogt, M., Marchıanı, J.L. Fischer ve M. Aragno, "Isolation of Thermus Strains from Hot Composts (60 to 80°C)", Applied and Environmental Microbiology, sayı 62, pp. 1723-1727, 1996.
- J. Sambrook ve D.W. Russell, "Molecular Cloning: A Laboratory Manual (3. Basım)", New York, USA: Cold Spring Harbor Laboratory Press, 2001.
- A. Çoşkun, "Endüstriyel Enzimler Üreten Yeni Bacıllus sp. Suşlarının İzolasyonu ve Karakterizasyonu", Yüksek Lisans Tezi. Çukurova Üniversitesi, Fen Bilimleri Enstitüsü, Adana, 2010.
- S. Okay, "Cloning of chitinase a gene (chıa) from Serratıa marcescens Bn10 and ıts expressıon ın coleoptera-specıfıc Bacıllus thurıngıensıs", Yüksek Lisans Tezi. Middle East Technical University, Ankara, 2005.
- W. Kugiyama, Y. Otani, Y. Hashimoto ve Y. Tagagi, "Molecular Cloning and Nucleotide Sequence of Lipase Gene", Biochemical and Biophysical Research Communications, sayı 14, pp. 185-190, 1980.
- E. Haba, O. Bresko, C. Ferrer, A. Marqués, M. Busquets ve A. Manresa, "Isolation of Lipase- Secreting Bacteria by Deploying Selective Substrate", Enzyme and Microbial Technology, sayı 26, pp. 40-44, 2000.
- D., Litthauer, A. Ginster ve E.V.E. Skein, "Pseudomonas luteola lipase: A new member of the 320-residue Pseudomonas lipase family", Enzyme and Microbial Technology, sayı 30, pp. 209-215, 2002.
- A. Altan, "Isolation and Molecula Characterization of Extracellular Lipase and Pectinase Producing Bacteria from Olive Oil Mills", Yüksek lisans Tezi, 2004.
- O.A.G. Ali, "Formation of proteases by Aspergillus fumigatus and pencillium sp.", Journal of King Saud University, sayı 4, pp. 127- 136, 1992.
- V.B.D., Skerman, V. McGowan ve P.H.A. Sneath, Approved lists of bacterial names. Washington, DC: American Society for Microbiology, 1989.
- E. Berline, Über die Schlaffsucht der mehlmottenraupe (Ephestiakühniella Zell) und ihren erreger Bacillus thuringiensis n. sp., Zeitschrift fur ange-wandte Entomologie Berlin 2, 29-56, 1915.
- L.K. Nakamura, "Bacillus pseudomycoides sp nov.", International Journal of Systematic and Evolutionary Microbiology, sayı 48, pp. 1031- 1035, 1998.
- S. Lechner, R. Mayr, K.P. Francis, B.M. Prüss, T. Kaplan, E. Wiessner-Gunkel, G.S. Stewart ve S. Scherer, "Bacillus weihenstephanensis sp nov. is a New Psychrotolerant Species of the Bacillus cereus Group", International Journal of Systematic Bacteriology, sayı 48, pp. 1373-1382, 1998.
- M.-H. Guinebretière, S. Auger, N. Galleron, M. Contzen, B. De Sarrau, M.-L. DeBuyser, G. Lamberret, A. Fagerlund, P.E. Granum, D. Lereclus, P. De Vos, C. Nguyen-The ve A. Sorokin, "Bacillus cytotoxicus sp nov. is a New Thermo-tolerant Species of the Bacillus cereus Group Occasionally Associated with Foodpoisoning", International Journal of Systematic and Evolutionary Microbiology, sayı 63, pp. 31-40, 2013.
- S. Shivaji, P. Chaturvedi, Z. Begum, P. K. Pindi, R. Manorama, D.A. Padmanaban, Y.S. Shouche, S. Pawar, P. Vaishampayan, C.B.S. Dutt, G.N. Datta, R.K. Manchanda, U.R. Rao, P.M. Bhargava ve J.V. Narlikar, "Janibacter hoylei sp. nov., Bacillus isronensis sp. nov. and Bacillus aryabhattai sp. nov., Isolated from Cryotubes Used for Collecting Air from the Upper Atmosphere", International Journal of Systematic and Evolutionary Microbiology, sayı 59, pp. 2977-2986, 2009
- J.R. Tagg, A.S. Dajani ve W.L. Wannamaker, "Bacteriocins of Gram-Positive Bacteria", Bacteriology Reviews, sayı 40, pp. 722-756, 1976.
- C., Le Marrec, B., Hyronimus, P., Bressollier, B., Verneuil, M.C., Urdaci, "Biochemical and Genetic Characterization of Coagulin, a New Antilisterial Bacteriocin in the Pediocin by Bacillus coagulans I(4)", Applied and Environmental Microbiology, sayı 66, pp. 5213- 5220, 2000.
- G. Zheng ve M.F. Slavik, "Isolation, Partial Purification and Characterisation of a Bacteriocin Produced by a Newly Isolated Bacillus subtilis strain", Letters in Applied Microbiology, sayı 28, pp. 363-367, 1999.
- P. Pattnaik, J.K. Kaushik, S. Grover ve V.K. Batish, "Purification and Characterization of a Bacteriocin-like Compound (Lichenin) Produced Anaerobically by Bacillus licheniformis Isolated from water buffalo", Journal of Applied Microbiology, sayı 91, pp. 636-645, 2001.
- A. Cherif, H. Ouzari, D. Daffonchio, H. Cherif, K. Ben Slama, A. Hassen, S. Jaoua ve A. Boudabous, "Thuricin 7: a Novel Bacteriocin Produced by Bacillus thuringiensis BMG1.7, a New Strain Isolated from Soil", Letters in Applied Microbiology, sayı 32, pp. 243-247, 2001.
- M. J., Waites, N.L., Morgan, J.S. Rockey ve G. Higton, "Industrial Microbiology an Introduction", London: Blackwell Publisher, 2008.
- G. Wei ve J.W. Kloepper ve S. Tuzun, "Induction of Systemic Resistance of Cucumber to Colletotrichum orbiculare by Select Strains of Plant Growth-promoting Rhizobacteria", Phytopathology, sayı 81, pp. 1508-1512, 1991.
- C. Chen, E.M. Bauske, G. Musson, R. Rodríguez-Kábana ve J.W. Kloepper,"Biological Control of Fusarium wilt on Cotton by Use of Endophytic Bacteria", Biological Control sayı 5, pp. 83-91, 1995.
- L.A. Silo-Suh, B.J. Lethbridge, S.J. Raffel, H. He, J. Clardy ve J. Handelsman, "Biological Activities of Two Fungistatic Antibiotics Produced by Bacillus cereus UW85", Applied and Environmental Microbiology, sayı 60, 2023- 2030, 1994.
- E.V. Stabb, L.M. Jacobson ve J. Handelsman, "Zwittermicin A-Producing Strains of Bacillus cereus from Diverse Soils", Applied and Environmental Microbiology, sayı 60, pp. 4404- 4412, 1994.
- O. Asaka ve M. Shoda, "Biocontrol of Rhizoctonia solani Damping-off of Tomato with Bacillus subtilis RB14", Applied and Environmental Microbiology, sayı 62, pp. 4081- 4085, 1996.