Tiyoredoksin Redüktaz Enzim Aktivitesi Üzerine Bal, Polen, Propolis ve Arı Sütü Extraktlarının İnhibisyon Etkilerinin Araştırılması

Tiyoredoksin redüktaz enzimi, apoptoz mekanizmasının çalışmasını önleyen ve böylece kanser oluşumunu tetikleyen bir enzimdir. Bu nedenle tiyoredoksin redüktaz enziminin inhibisyonunun kanseri önlediği veya durdurduğu düşünülmektedir. Bu çalışmada, yayla balının, çam balının, kestane balının, deli veya yabani balının, polenin, propolisin ve arı sütünün ekstraktlarının tioredoksin redüktaz enzim aktivitesi üzerindeki etkileri araştırılmıştır. Enzim aktiviteleri IC50 değerlerini hesaplamak için sabit substrat ve farklı inhibitör konsantrasyonlarında ölçülmüştür. İnhibisyon sabitinin ölçümü için beşten fazla farklı inhibitör konsantrasyonları kullanılmıştır. İnhibisyon çalışmasında kullanılan ekstraktları karşılaştırmak için toplam antioksidan aktivitesi araştırılmıştır. En güçlü inhibisyon etkisi polen metanol ekstraktında (IC50 = 2.44 μg/mL) görülmüştür.

Anahtar Kelimeler:

kanser, arı ürünleri

An Investigation of Inhibition Effects of Honey, Pollen, Propolis and Royal Jelly Extracts on Thioredoxin Reductase Enzyme Activity

The thioredoxin reductase enzyme is an enzyme that prevents the mechanism of apoptosis from working and thus triggers the formation of cancer. Therefore, the inhibition of the thioredoxin reductase enzyme is thought to prevent or inhibit cancer. In this study, the effects of extracts of plateau honey, pine honey, chestnut honey, mad or wild honey, pollen, propolis and royal jelly on thioredoxin reductase enzyme activity were investigated. Enzyme activities were measured at constant substrate and different inhibitor concentrations to calculate IC50 values. Over five different inhibitor concentrations were used for measuring the inhibition constant. Total antioxidant activity were investigated in order to compare the extracts used in the inhibition study. The strongest inhibitory effect was seen in the pollen methanol extract (IC50 = 2.44μg /mL)

Keywords:

bee products Enzyme Activity, Honey,

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