In this study, effects of combination of pectinase and cellulase on the removal of grapefruit membrane were searched. Comparisons were made with chemical (NaOH) method; considering the quality of final product and applicability in the industry. On the removal of segment membrane, both the concentration of Pectinex Ultra SpL and Celluclast 1.5 L and treatment period were found to be effective. In the process of removal of segment membrane by enzyme solutions and NaOH, the best results were obtained by using of Pectinex Ultra SpL at 2% and Celluclast 1.5 L at 1% concentrations and 30 min. treatment. Segments peeled by enzymatic treatments were found to be superior organoleptically over the segments of NaOH treatment.

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