Merve Nur İNCE, Asuman DEVECİ ÖZKAN, Erhan BEZDEGÜMELİ, Ahmet Yasir MEN, Bilge Nur KÜÇÜKAKÇA, Gamze GÜNEY ESKİLER

MEG3’ün Prostat Kanseri Hücrelerindeki Toll Benzeri Reseptör 3 Aktivasyonundaki Rolü

Amaç: Dünya genelinde erkeklerdeki yeni kanser tanılarının yaklaşık %10'unu prostat kanseri oluşturmaktadır. Toll-benzeri reseptörler (TLR), prostat kanseri gelişiminde önemli rol oynamaktadır. Ayrıca TLR’lerin ekspresyon seviyesi çeşitli transkripsiyon faktörleri ve kodlanmayan RNA’lar ile düzenlenmektedir. Bu nedenle bu çalışmada MEG3’ün potansiyel düzenleyici rolünün ve TLR3-MEG3 ilişkisinin belirlenmesi amaçlanmaktadır. Gereç ve Yöntemler: Bu çalışmada PC-3, LNCaP ve HUVEC hücreleri kullanılmıştır. Poli I:C; bir TLR3 ligandı olarak TLR3 ekspresyonunu uyarmak için kullanılmıştır. Poli I:C' nin toksik olmayan konsantrasyonu WST-1 analizi ile belirlenmiştir. TLR3 ve MEG3'ün relatif gen ekspresyon seviyeleri RT-PCR ile analiz edilmiştir. Bulgular: Sonuçlara göre, 5 µM Poli I:C TLR3’ü aktive edilmesi için toksik olmayan konsantrasyon olarak seçilmiştir. MEG3 (3.19-, 1.90- ve 1.90-kat) ve TLR3 (6.17-, 5.75- ve 2.27-kat) mRNA seviyelerinin Poli I:C uygulamasından sonra sırasıyla PC-3, LNCaP ve HUVEC hücrelerinde anlamlı bir şekilde arttığı belirlenmiştir (p

Anahtar Kelimeler:

Prostat kanseri, TLR3, MEG3, Poli I:C

The Role of MEG3 in the Activation of Toll Like Receptor 3 in Prostate Cancer Cells

Objective: Prostate cancer accounts for approximately 10% of new cases diagnosed in men worldwide. Toll like receptors (TLRs) play a crucial role in the progression of cancer. Furthermore, the expression level of TLRs is mediated by different transcription factors and non-coding RNAs. Therefore, the aim of this study was to investigate the potential regulatory role of MEG3 and the interaction of TLR3 with MEG3 in the prostate cancer cells. Materials and Methods: In this study, PC-3, LNCaP and HUVEC cells were used. To stimulate TLR3 expression, Poly I:C was used for a ligand of TLR3 and the less cytotoxic concentration of Poly I:C was determined by WST-1 analysis. The relative gene expression levels of TLR3 and MEG3 were analyzed by RT-PCR. Results: According to the results, 5 µM of Poly I:C was chosen as a less cytotoxic concentration for the stimulation of TLR3 activity. The mRNA level of MEG3 (3.19-, 1.90-, and 1.90-fold) and TLR3 (6.17-, 5.75- and 2.27-fold) was significantly increased in PC-3, LNCaP and HUVEC cells, respectively after Poly I:C stimulation (p

Keywords:

Prostate cancer, TLR3, MEG3, Poly I:C,

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