Statik Manyetik Alanın Trombosit Agregasyonuna Etkisi
Amaç: Trombositler, salgıladıkları maddeler ve membran proteinleri ile birlikte koagülasyon ve inflamasyonda önemli rol oynayan 4-7 Mikron çapında kan hücreleridir. Manyetik alanın günümüzde, canlılara ve kan hücrelerine etkilerini araştıran birçok çalışma olmakla birlikte henüz etki mekanizması tam olarak açıklanamamıştır. Özellikle düşük sabit manyetik alanın lökositleri aktive ettiği ve sayılarını arttırdığı tespit edilmiştir fakat trombositler üzerindeki etkileri ile ilgili tartışmalı veriler mevcuttur. Bu çalışmada sabit manyetik alanın trombosit fonksiyonları üzerindeki etkilerini incelemeyi amaçladık. Gereç ve Yöntem: Trombositler farklı şiddetlerdeki (2mT, 5mT, 40mT) manyetik alana, farklı sürelerde (5, 15 ve 30 dakika) maruz bırakıldı ve trombosit agregasyonları farklı zaman aralıklarında (0, 24, 40, 48 saat) agregometre kullanılarak tespit edildi. ADP uyaranı kullanılarak trombosit agregasyon kurbu eğim ve % amlitüd olarak belirlendi. Bulgular: 5mT’da 15dk ve 30dk inkübasyondan sonra 24. saatteki trombosit agregasyonları, kontrole göre daha yüksek bulundu (eğim p=0,008, amplitüd p=0,026). 15 ve 30 dakika arasında ise istatistiksel olarak anlamlı bir fark saptanmadı. 2mT’da 15 ve 30 dakika inkübasyondan sonra 48. saatteki agregasyonları kontrole göre anlamlı olarak yüksek bulundu (p=0,038). 2mT’daki trombosit agregasyonu, 5mT’daki trombosit agregasyonundan istatistiksel olarak daha yüksek bulundu (p=0,032). Sonuç: Manyetik alana maruz kalan trombositlerde agregasyon artışı olduğu tespit edildi. 2mT’lık statik manyetik alan maruziyetinin 48 saat sonra bile trombosit agregasyon yanıtını olumlu etkilemesi, bu etkinin trombositlerin vücut dışında daha uzun süre aktivitelerini korumalarını sağlayacağını düşündürmektedir.
The Effect of Static Magnetic Field on Platelet Aggregation
Objective: Platelets are 4-7 Micron diameter blood cells that play an important role in coagulation and inflammation with the substances they secrete and the membrane proteins. Although there are many studies investigating the effect of a magnetic field on living organisms and blood cells, it’s mechanism has not been clarified yet. It has been demonstrated that the low-intensity magnetic field activates the leukocytes and increases their number, but there are controversial data regarding its effect on platelets. In this study, we aimed to examine the effects of a magnetic field on platelet functions. Materials and Methods: Platelets were exposed to different intensities of magnetic field (2mT, 5mT, 40mT) at different times (5, 15 and 30 minutes) and the aggregation of platelets was detected at different time intervals (0, 24, 40, 48 hours) using the aggregometer.The platelet aggregation curve was determinated as slope and amlitude (%). Results: After a 15´ and 30´ incubation at 5mT, platelet aggregation at the 24th hour was found to be higher than the control (Slope p=0.008, Amplitude p=0.026). There was no statistical difference between 15 and 30 minutes. The 48th hour aggregation was found to be significantly higher than the control after a 15´ and 30´ incubation at 2mT (p=0.038). Platelet aggregation at 2mT was higher than platelet aggregation at 5mT (p=0.032). Conclusion: It was determined that there was an increase in aggregation of platelets exposed to a magnetic field. The fact that 2mT static magnetic field exposure positively affects the platelet aggregation even after 48 hours, suggests that this effect will enable the platelets to maintain their activities outside of the body for a longer time.
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